Preliminary DNA fingerprinting of the turf grass &lt;i&gt;Cynodon dactylon&lt;/i&gt;  (Poaceae: Chloridoideae)

Roodt, Rouvay; Spies, J. J.; Burger, T. H.

doi:10.4102/abc.v32i1.474

Cited by 29 publications

(15 citation statements)

References 32 publications

(11 reference statements)

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“…For instance, RAPD provides a simple PCR-based molecular tool for the evaluation of genetic variation, but its poor consistency and low reproducibility limit its use (Welsh and McClelland 1990;Roodt et al 2002). Amplified fragment length polymorphism (AFLP) technology is now widely used for genomic fingerprinting (Zhang et al 1999;Karaca et al 2002) due to its high multiplexing ratio (Vos et al 1995).…”

Section: Introductionmentioning

confidence: 98%

Molecular characterization of Buffalograss germplasm using sequence-related amplified polymorphism markers

et al. 2003

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Buffalograss [ Buchloe dactyloides (Nutt.) Englem] germplasm has a broad resource of genetic diversity that can be used for turfgrass, forage and conservation. Buffalograss is the only native grass that is presently used as a turfgrass in the Great Plains region of North America. Its low growth habit, drought tolerance and reduced requirement for fertilizer and pesticides contribute to interest in its use. The objectives of this study were to use sequence-related amplified polymorphism (SRAP) markers in the evaluation of genetic diversity and phenetic relationships in a diverse collection of 53 buffalograss germplasms, and to identify buffalograss ploidy levels using flow cytometry. Based on their DNA contents, buffalograss genotypes were grouped into four sets, corresponding to their ploidy levels. Thirty-four SRAP primer combinations were used. This is the first report of the detection of differentiating diploid, tetraploid, pentaploid and hexaploid buffalograss genotypes, representing diverse locations of origin, using SRAP markers. Cluster analysis by the unweighted pair-group method with arithmetic averages based on genetic similarity matrices indicated that there were eight clusters. The coefficients of genetic distance among the genotypes ranged from 0.33 up to 0.99 and averaged D=0.66. The genetic diversity estimate, He, averaged 0.35. These results demonstrated that genotypes with potential traits for turfgrass improvement could readily be distinguished, based on SRAP. The use of PCR-based technologies such as SRAP is an effective tool for estimating genetic diversity, identifying unique genotypes as new sources of alleles for enhancing turf characteristics, and for analyzing the evolutionary and historical development of cultivars at the genomic level in a buffalograss breeding program.

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Section: Introductionmentioning

confidence: 98%

Molecular characterization of Buffalograss germplasm using sequence-related amplified polymorphism markers

et al. 2003

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“…Modern techniques using DNA makers have been extensively used in studies of the genetic diversity of C. dactylon. Roodt et al (2002) used the random amplified polymorphic DNA (RAPD) technique to determine the genetic relationship among C. dactylon cultivars in the South Africa. Caetano-Anollés et al (1997) and Caetano-Anollés (1998) used DNA fingerprinting techniques to investigate the genetic relationships among C. dactylon and the DAF method to analyze the genomes of 2 varieties of Tifgreen and Tifdrawf.…”

Section: Introductionmentioning

confidence: 99%

Analysis of genetic diversity among wild bermudagrass germplasm from southwest China using SSR markers

Yao¹,

Zhang²,

Ma³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Fifty-five wild accessions of bermudagrass (Cynodon dactylon) were collected from southwest China (Sichuan, Chongqing, Yunnan, Guizhou, and Tibet), and their genetic diversity was analyzed using simple sequence repeat markers. A total of 267 polymorphic bands were detected with 18 primer combinations. The genetic similarity among the accessions ranged from 0.688 to 0.894 with an average of 0.797. All 55 wild accessions were clustered into 7 ecogeographic groups. Our data showed that the dendrogram was almost in accordance with geographic distribution, and accessions from the same collection sites tended to be clustered into the same group. A genetic differentiation analysis revealed that the percentage of genetic variance was 70.07 and 29.93% within and among groups, respectively. Finally, we discuss the implications of these results for C. dactylon in southwest China.

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“…Isoenzyme electrophoresis was used to identify clonal forage bermudagrass cultivars (Dabo et al, 1990). In recent years, DNA markers, including DNA amplifi ed fi ngerprinting (DAF) (Caetano-Anolles et al, 1995;Ho et al, 1997;Anderson et al, 2001;Yerramsetty et al, 2005), random amplifi ed polymorphic DNA (RAPD) (Roodt et al, 2002), amplifi ed fragment length polymorphism (AFLP) (Zhang et al, 1999;Capo-chichi et al, 2005), and sequence-related amplifi ed polymorphism (SRAP) (Wang et al, 2009) have been used to evaluate and characterize bermudagrass genetic diversity and relatedness. In recent years, DNA markers, including DNA amplifi ed fi ngerprinting (DAF) (Caetano-Anolles et al, 1995;Ho et al, 1997;Anderson et al, 2001;Yerramsetty et al, 2005), random amplifi ed polymorphic DNA (RAPD) (Roodt et al, 2002), amplifi ed fragment length polymorphism (AFLP) (Zhang et al, 1999;Capo-chichi et al, 2005), and sequence-related amplifi ed polymorphism (SRAP) (Wang et al, 2009) have been used to evaluate and characterize bermudagrass genetic diversity and relatedness.…”

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confidence: 99%

Identification of Vegetatively Propagated Turf Bermudagrass Cultivars Using Simple Sequence Repeat Markers

Wang

Martin

et al. 2010

Crop Science

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Accurate identification of bermudagrass (Cynodon spp.) cultivars is necessary to ensure the purity of the cultivars produced by sod farmers, to protect the intellectual property of cultivar developers, and to assure cultivar purity for the benefit of turfgrass consumers. Vegetatively propagated turf bermudagrass cultivars have been extensively used in the turf industry not only in the USA but also in many other countries. Accordingly, the objectives of the study were to examine simple sequence repeat (SSR) markers for their ability to distinguish commonly grown clonal turf bermudagrass cultivars, which were derived through crosses and mutations, from each other and their respective parent cultivars and to develop a set of SSR markers for accurate identification of commercially used clonal cultivars. Thirty‐two clonal turf bermudagrass genotypes comprising 29 commercially released cultivars and 3 Oklahoma State University experimental lines were assessed by 11 microsatellite markers. A total of 141 DNA fragments were generated for the 11 primer pairs in the 32 bermudagrass genotypes, with an average of 12.8 bands per primer pair. Forty‐four fragments were cultivar specific. The SSR markers successfully identified 22 cultivars when mutant cultivars had the same banding patterns as the 2 parent cultivars, ‘Tifgreen’ and ‘Tifway’. It was concluded from the study that the SSR markers are highly polymorphic and can be utilized as a reliable tool for accurate cultivar identification in nonmutated bermudagrass.

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Preliminary DNA fingerprinting of the turf grass <i>Cynodon dactylon</i> (Poaceae: Chloridoideae)

Cited by 29 publications

References 32 publications

Molecular characterization of Buffalograss germplasm using sequence-related amplified polymorphism markers

Molecular characterization of Buffalograss germplasm using sequence-related amplified polymorphism markers

Analysis of genetic diversity among wild bermudagrass germplasm from southwest China using SSR markers

Identification of Vegetatively Propagated Turf Bermudagrass Cultivars Using Simple Sequence Repeat Markers

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