Preliminary crystallographic studies of purine nucleoside phosphorylase from the cariogenic pathogenStreptococcus mutans

Abstract

The punA gene of the cariogenic pathogen Streptococcus mutans encodes purine nucleoside phosphorylase (PNP), which is a pivotal enzyme in the nucleotidesalvage pathway, catalyzing the phosphorolysis of purine nucleosides to generate purine bases and -ribose 1-phosphate. In the present work, the PNP protein was expressed in Escherichia coli strain BL21 (DE3) in a soluble form at a high level. After purification of the PNP enzyme, the protein was crystallized using the sitting-drop vapour-diffusion technique; the crystals diffracted to 1.6 Å resolution at best. The crystals belonged to space group H3, with unit-cell parameters a = b = 113.0, c = 60.1 Å .

NupR Responding to Multiple Signals Is a Nucleoside Permease Regulator in Bacillus thuringiensis BMB171

“…In addition, purine metabolism and transport are influenced by the deletion of NupR. The change in the expression of the following genes, pbuG (BMB171_RS20290), pyn (BMB171_RS20300), punA (BMB171_RS20305), deoB (BMB171_RS20310), and BMB171_RS16385 coding for extracellular RNase ( 9 – 12 ), may be caused by the alteration of nucleoside transport in Δ nupR . Also, vitamin B 12 participates in DNA, fatty acid, and amino acid synthesis.…”

Potential therapeutic drug target identification in Community Acquired-Methicillin Resistant Staphylococcus aureus (CA-MRSA) using computational analysis

“…This enzyme is also involved in pyrimidine, nicotinate and nicotinamide metabolism. PNP has been reported as potential therapeutic drug target in M. tuberculosis and Streptococcus mutans [ 24 , 25 ].…”