Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal

Filipe, Diogo; Parreira, Ricardo; Pereira, André; Galvão, Nuno; Cristóvão, José Manuel; Nunes, Mónica; Vieira, Maria Luísa; Campino, Lenea; Maia, Carla

doi:10.1016/j.vprsr.2021.100551

Cited by 2 publications

(4 citation statements)

References 25 publications

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“…Due to the presence of similarities and juvenile stages, it is inadequate to achieve the accurate morphological identification of tick species. Therefore, genetic markers such as mitochondrial cox1 gene have been successfully proven as reliable for the molecular confirmation of different ticks ( Filipe et al., 2021 ; Zhao et al, 2020 ). In the current study, we report the Hy.…”

Section: Discussionmentioning

confidence: 99%

Genotyping of ticks: first molecular report of Hyalomma asiaticum and molecular detection of tick-borne bacteria in ticks and blood from Khyber Pakhtunkhwa, Pakistan

Obaid,

Shehla,

Guan

et al. 2024

Front. Cell. Infect. Microbiol.

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Multiple ticks (Acari: Ixodoidea) carrying Rickettsiales bacteria have significant importance for both human and animal health. Thus, the purpose of this work was to genetically analyze tick species and their associated Rickettsiales bacteria in animal hosts. In order to achieve these objectives, various animals (including camels, cattle, goats, sheep, dogs, and mice) were inspected in four districts (Mardan, Peshawar, Kohat, and Karak) of Khyber Pakhtunkhwa to collect ticks, while blood samples were collected from all the symptomatic and asymptomatic cattle in all four districts. A total of 234 ticks were obtained from 86 out of 143 (60.14%) host animals, which were morphologically identified as Rhipicephalus turanicus, Rhipicephalus microplus, Haemaphysalis cornupunctata, and Hyalomma asiaticum. Among these, their representative ticks (126/234, 53.85%) were processed for molecular confirmation using cytochrome c oxidase (cox1) gene. Obtained cox1 sequences of four different tick species showed 99.72%–100% maximum identity with their corresponding species reported from Pakistan, China, India, and Kazakhstan and clustered phylogenetically. This study presented the first genetic report of Hy. asiaticum ticks in Pakistan. Moreover, genetically confirmed tick species were molecularly analyzed by PCR for detection of Rickettsiales DNA using partial fragments of 16S rDNA, 190-kDa outer membrane protein A (ompA), and 120-kDa outer membrane protein B (ompB) genes. In addition, blood samples were analyzed to identify Rickettsiales bacteria using the aforementioned genes. Rickettsiales bacteria were found in 24/126 (19.05%) ticks and 4/16 (25.00%) in symptomatic cattle’s blood. The obtained ompA and ompB sequences from Hy. asiaticum ticks showed 99.73%–99.87% with Candidatus Rickettsia shennongii and unidentified Rickettsia sp., whereas the obtained 16S rDNA sequences from cattle’s blood and ticks (Hae. cornupunctata) showed 99.67% highest identity with Anaplasma phagocytophilum. The 16S rDNA sequence of Rickettsiales DNA from Rh. turanicus ticks showed 100% identity with Ehrlichia canis and unidentified Ehrlichia sp. Obtained sequences of Rickettsiales bacteria were grouped along with their respective species in phylogenetic trees, which were previously reported in Greece, Cuba, Iraq, Turkey, Pakistan, South Korea, and China (mainland and Taiwan). This extensive study explores the wide range of damaging ticks and their corresponding tick-borne bacteria in the area, suggesting a possible danger to both livestock and human communities.

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Section: Discussionmentioning

confidence: 99%

Genotyping of ticks: first molecular report of Hyalomma asiaticum and molecular detection of tick-borne bacteria in ticks and blood from Khyber Pakhtunkhwa, Pakistan

Obaid,

Shehla,

Guan

et al. 2024

Front. Cell. Infect. Microbiol.

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“…Cox1 gene appears to be more useful for intraspecific genetic variability studies (Filipe et al, 2021;Paternina et al, 2016). This highlights the difficulty of using these two genes and the need for more genetic characterization using whole-genome sequencing (Burger et al, 2014;Low et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…microplus clade C. Hence, the morphological classification obtained may not always reflect the molecular results (Nava et al, 2009). In this study where we have used both Cox1 and 16S rDNA as well as morphologic identification keys for the taxonomic assignation of ticks, the species was determined by an identical outcome with at least two of the three tools used as shown elsewhere (Filipe et al, 2021; Paternina et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…microplus showing that 16S rDNA gene is more conserved. It has been shown elsewhere that 16S rDNA gene seems to have better features for interspecific phylogenetic analyses while Cox1 gene appears to be more useful for intraspecific genetic variability studies (Filipe et al, 2021; Paternina et al, 2016). This highlights the difficulty of using these two genes and the need for more genetic characterization using whole‐genome sequencing (Burger et al, 2014; Low et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

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Infestation rates, seasonal distribution, and genetic diversity of ixodid ticks from livestock of various origins in two markets of Yaoundé, Cameroon

Yousseu

Tchetgna

Kamgang

et al. 2022

Medical Vet Entomology

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Little is known about the impact of ticks on livestock and humans in Cameroon. This study aimed to determine the prevalence, seasonal variation, and genetic diversity of hard ticks in the country. Ticks were collected during a cross-sectional survey on domestic livestock in two markets of Yaoundé in 2019 and 2020 and identified using morphological keys, 16S ribosomal DNA, (16S rDNA), and the cytochrome c oxidase subunit 1 (Cox1) genes.The infestation rates were 39.18%, 11.53%, and 2.74% in cattle, sheep, and goats respectively. Three genera of ticks were identified, Rhipicephalus, Amblyomma, and Hyalomma comprising eleven tick species. The main species were Rhipicephalus decoloratus (30.25%), R. microplus (24.43%), and Amblyomma variegatum (12.96%). Rhipicephalus spp. (81.31%) and Amblyomma variegatum (51.54%) were abundant during the rainy season, while Hyalomma spp. (83.86%) during the dry season (p-value <0.00001). Cox1 and 16S rDNA analysis showed a high level of genetic diversity among tick species with sequences close to those observed across Africa. Phylogenetic analysis revealed that our R. microplus belong to clade A and we identified R. sanguineus s.l. as R. linnea. This study shows a high tick infestation rate in cattle, while low in small ruminants with an extensive diversity of tick species, including several known vectors of important tick-borne diseases.

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Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal

Cited by 2 publications

References 25 publications

Genotyping of ticks: first molecular report of Hyalomma asiaticum and molecular detection of tick-borne bacteria in ticks and blood from Khyber Pakhtunkhwa, Pakistan

Genotyping of ticks: first molecular report of Hyalomma asiaticum and molecular detection of tick-borne bacteria in ticks and blood from Khyber Pakhtunkhwa, Pakistan

Infestation rates, seasonal distribution, and genetic diversity of ixodid ticks from livestock of various origins in two markets of Yaoundé, Cameroon

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