Preliminary biochemical studies on a model growing of different tobacco plant (Nicotiana tabacum L.) cultivars

Szedljak, I.; Kőhegyi, K. Szántainé; Kosary, J.

doi:10.31421/ijhs/13/2/729

Cited by 2 publications

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“…Tomato fruits are edible but contain relatively low in protein content (0.26%–0.47%; Adebooye et al ., ). Tobacco may provide the highest crude protein content in leaves (7–69 mg/g of dry weight; Szedljak et al ., ), but the toxic alkaloids such as nicotine are not appropriate for oral delivery (Mason et al ., ). Moreover, banana has a high yield of total biomass with a maximum residual biomass (13–20 metric tons dry matter/ha) in pseudostem and leaves, but tobacco plants can only produce 2.2 metric tons of biomass per hectare (Fischer and Emans, ).…”

Section: Discussionmentioning

confidence: 99%

Oral immunogenicity of porcine reproductive and respiratory syndrome virus antigen expressed in transgenic banana

Chan

Chia

Pang

et al. 2012

Plant Biotechnology Journal

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SummaryPorcine reproductive and respiratory syndrome virus (PRRSV) is a persistent threat of economically significant influence to the swine industry worldwide. Recombinant DNA technology coupled with tissue culture technology is a viable alternative for the inexpensive production of heterologous proteins in planta. Embryogenic cells of banana cv. 'Pei chiao' (AAA) have been transformed with the ORF5 gene of PRRSV envelope glycoprotein (GP5) using Agrobacterium-mediated transformation and have been confirmed. Recombinant GP5 protein levels in the transgenic banana leaves were detected and ranged from 0.021%-0.037% of total soluble protein. Pigs were immunized with recombinant GP5 protein by orally feeding transgenic banana leaves for three consecutive doses at a 2-week interval and challenged with PRRSV at 7 weeks postinitial immunization. A vaccination-dependent gradational increase in the elicitation of serum and saliva anti-PRRSV IgG and IgA was observed. Furthermore, significantly lower viraemia and tissue viral load were recorded when compared with the pigs fed with untransformed banana leaves. The results suggest that transgenic banana leaves expressing recombinant GP5 protein can be an effective strategy for oral delivery of recombinant subunit vaccines in pigs and can open new avenues for the production of vaccines against PRRSV.

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Section: Discussionmentioning

confidence: 99%

Oral immunogenicity of porcine reproductive and respiratory syndrome virus antigen expressed in transgenic banana

Chan

Chia

Pang

et al. 2012

Plant Biotechnology Journal

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Biochemical Studies on Curing and Fermentation Processing Periods of Different Tobacco Plant (Nicotianatabacum L.) Cultivars

Szedljak

Koehegyi

Kosary

2010

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

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SUMMARYWe studied the changes in the activity of the enzymes polyphenol oxidase (PPO) and peroxidase (POX), the concentration of total soluble phenolic compounds and soluble protein content in different tobacco cultivars (Virginia and Burley) during curing. The curing procedure was a special combination of open-air-curing and fluecuring methods and a long fermentation period. In the curing period the changes in data suggested a combination of the biochemical processes and the direct effect of oxygen. A slight increase then a decrease in the concentrations of both total soluble phenolic compounds and the soluble protein content were detected. In this period we found no correlation between the concentration of total soluble phenolic compounds, the decreasing PPO and the increasing POX activity. In the fermentation period a deactivation of the enzymes (PPO and POX) and a decrease in the concentration of both total soluble phenolic compounds and soluble protein content were found, because the fermentation is not a biochemically-regulated process. Moreover, these results suggest that the end of curing period is the most favourable time for protein isolation from different tobacco cultivars. [Beitr. Tabakforsch. Int. 24 (2010) 24-28]

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Preliminary biochemical studies on a model growing of different tobacco plant (Nicotiana tabacum L.) cultivars

Cited by 2 publications

References 0 publications

Oral immunogenicity of porcine reproductive and respiratory syndrome virus antigen expressed in transgenic banana

Oral immunogenicity of porcine reproductive and respiratory syndrome virus antigen expressed in transgenic banana

Biochemical Studies on Curing and Fermentation Processing Periods of Different Tobacco Plant (Nicotianatabacum L.) Cultivars

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