Preliminary assessment on Agrobacterium-mediated Transformation of Dendrobium Broga Giant orchids PLBs

Uddain, Jasim; Zakaria, Latiffah; Lynn, Chew Bee; Subramaniam, Sreeramanan

doi:10.9755/ejfa.2015.05.211

Cited by 6 publications

(2 citation statements)

References 25 publications

(31 reference statements)

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“…of the Agrobacterium are the most essential steps during a transformation event (Merritt et al, 2007;Uddain et al, 2015). Many studies have taken explant wounding as a routine practice presumably because vir inducers were produced at the host plant wounded sites which acted as chemo-attractants for Agrobacterium attachment (Simoh et al, 2007, Zia et al, 2010.…”

Section: Discussionmentioning

confidence: 99%

Efficient regeneration and Agrobacterium-mediated transformation protocol for recalcitrant indica rice (Oryza sativa L.).

Wong

Lai

Wong

et al. 2015

Emir. J. Food Agric

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Section: Discussionmentioning

confidence: 99%

Efficient regeneration and Agrobacterium-mediated transformation protocol for recalcitrant indica rice (Oryza sativa L.).

Wong

Lai

Wong

et al. 2015

Emir. J. Food Agric

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“…The orchids, Dendrobium, Phalaenopsis, and Oncidium are commercial varieties, used extensively for genetic transformations; other novels and exotic varieties de ning socio-economic attributes need to be further studied for trait improvement (Li et al, 2021), de ning expansion of branch of genetic engineering for key studies. As a possible solution, PLBs were used in key orchid species Dendrobium (Uddain et al, 2015), Cymbidium (Yang et al, 1999), Phalaenopsis (Liao et al, 2004Chan et al, 2005), and Oncidium Li et al, 2005), as transformation receptors, with studies on best receptors in progress. Other major concerns in global oriculture trade comprise extensive usage of online platforms, contributing to illegal trade, and unsustainable harvesting of rare varieties (Hinsley A, 2018).…”

Section: Global Updates Success Stories and Associated Bottlenecksmentioning

confidence: 99%

Biotechnological interventions in Orchids: Recent updates, Translational success, and Commercial outcomes

Tiwari

Sharma

Bose

et al. 2022

Preprint

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Orchids constitute the largest and most diverse group of flowering plants and are classified in Orchidaceae. Exhibiting significance as the most exotic and ubiquitous flowering plants, the cultivation of orchids on a commercial level, is gaining momentum worldwide. In addition to its ornamental and aesthetic value, the orchid industry has been successful in generating employment for people in developing countries. Recent advances in biotechnological interventions in orchids have substantially contributed to the development of exotic varieties with novel traits, not to forget the inputs of traditional plant breeding methods and tissue culture approaches. In addition, the scientific developments in orchid biology have remarkably improved the knowledge in areas of orchid biology, classification, phytochemistry, and cultivation strategies, among other areas. This has facilitated the commercialization of novel varieties, opening new avenues in the orchid industry, witnessed as cultivation of different varieties and globally marketed as a cut flower and artificially propagated plants. The orchids constitute the first floriculture crops, which revolutionized the orchid industry, however, it also withholds several challenges in natural propagation, several species facing extinction. International organizations like CITES have come forward to address challenges associated with illegal global trade and indiscriminate use of orchid varieties, aiming towards conservation and legal commercial goals. This thematic review is one of a kind in providing comprehensive insights on the emerging momentum of orchid biology and how its globalization projects to considerably impact the orchid industry in the coming time. Together with traditional breeding approaches and plant tissue culture, advances in biotechnological interventions continues to make substantial progress in the development of exotic varieties, with multi-faceted attributes. However, it is imperative to understand the challenges in the cultivation and conservation of orchid varieties and ensure legislative guidelines both on domestic and global levels to ensure a multipronged approach for the conservation and commercialization of orchids.

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Methods for genetic transformation in Dendrobium

Silva

Dobránszki

Cardoso³

et al. 2016

Plant Cell Rep

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The genetic transformation of Dendrobium orchids will allow for the introduction of novel colours, altered architecture and valuable traits such as abiotic and biotic stress tolerance. The orchid genus Dendrobium contains species that have both ornamental value and medicinal importance. There is thus interest in producing cultivars that have increased resistance to pests, novel horticultural characteristics such as novel flower colours, improved productivity, longer flower spikes, or longer post-harvest shelf-life. Tissue culture is used to establish clonal plants while in vitro flowering allows for the production of flowers or floral parts within a sterile environment, expanding the selection of explants that can be used for tissue culture or genetic transformation. The latter is potentially the most effective, rapid and practical way to introduce new agronomic traits into Dendrobium. Most (69.4 %) Dendrobium genetic transformation studies have used particle bombardment (biolistics) while 64 % have employed some form of Agrobacterium-mediated transformation. A singe study has explored ovary injection, but no studies exist on floral dip transformation. While most of these studies have involved the use of selector or reporter genes, there are now a handful of studies that have introduced genes for horticulturally important traits.

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Preliminary assessment on Agrobacterium-mediated Transformation of Dendrobium Broga Giant orchids PLBs

Cited by 6 publications

References 25 publications

Efficient regeneration and Agrobacterium-mediated transformation protocol for recalcitrant indica rice (Oryza sativa L.).

Efficient regeneration and Agrobacterium-mediated transformation protocol for recalcitrant indica rice (Oryza sativa L.).

Biotechnological interventions in Orchids: Recent updates, Translational success, and Commercial outcomes

Methods for genetic transformation in Dendrobium

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