Abstract:Investigations were carried out in the main stone‐fruit growing areas of Lebanon to assess the phytosanitary condition of commercial orchards. The presence of virus and virus‐like diseases and their identification was ascertained through: (i) field surveys, (ii) sap transmission to herbaceous hosts, (iii) graft transmission to woody indicators; and (iv) ELISA and IEM tests. The mean infection level was 25%. ranging from 5% in apricot to 45% in cherry. The following viruses were identified: apple chlorotic leaf… Show more
“…Differenial transmission of individual viruses through rootstocks and, perhaps, pollen may account for these differences (Digiaro & Savino, 1992). ApMV, which was no1 detected in a previous survey (Jawhar et al, 1996), is reported for the first time in peach in Lebanon. The low incicence of ApMV in the country is remarkable, especially as this virus is not rare in the Mediterranean region (Savino e* al., 1995).…”
Section: As Shown Inmentioning
confidence: 75%
“…2), PLMVd was detected in 17 samples belonging to native and imported varieties (Babcock, Chikhani, Springtime, Nectarose, Dixiered) grown in all areas surveyed. (Jawhar et al, 1996). ACLSV was second in pxevalence, occurring in 27.1% of the surveyed orchards, followed by PDV (22.4%) and ApMV (2.1 %).…”
Field surveys were carried out in the main peach-growing areas of Lebanon to assess the presence and distribution of viruses and viroids in commercial orchards. Field inspections were made in spring and summer 2000 to observe symptoms of virus and viroid diseases respectively. In total, 950 trees in 95 commercial plantings from three different regions of Lebanon (Bekaa Valley, Mount Lebanon and north Lebanon) were surveyed and sampled. Immunoenzymatic tests (DAS-ELISA) were used to ascertain the presence of the following: Prunus necrotic ring spot ilarvirus (PNRSV), Prune dwarf ilarvirus (PDV), Apple mosaic ilarvirus (ApMV), Apple chlorotic leaf spot trichovirus (ACLSV), Plum pox potyvirus (PPV), Tomato ringspot nepovirus (ToRSV) and Strawberry latent ringspot nepovirus (SLRSV). Peach latent mosaic pelamoviroid (PLMVd) and Hop stunt hostuviroid (HSVd) were identified by molecular hybridization. About 25% of the tested samples were infected by one or more viruses. In particular, the prevailing virus was PNRSV (61.2% of infection), followed by ACLSV (27.1%), PDV (22.4%) and ApMV (2.1%). Mixed infections were about 13%. ToRSV, SLRSV and PPV were not found. HSVd was apparently absent, whereas PLMVd was identified in 34% of the samples examined. This viroid prevailed in certain areas of Mount Lebanon in both native and foreign cultivars.
“…Differenial transmission of individual viruses through rootstocks and, perhaps, pollen may account for these differences (Digiaro & Savino, 1992). ApMV, which was no1 detected in a previous survey (Jawhar et al, 1996), is reported for the first time in peach in Lebanon. The low incicence of ApMV in the country is remarkable, especially as this virus is not rare in the Mediterranean region (Savino e* al., 1995).…”
Section: As Shown Inmentioning
confidence: 75%
“…2), PLMVd was detected in 17 samples belonging to native and imported varieties (Babcock, Chikhani, Springtime, Nectarose, Dixiered) grown in all areas surveyed. (Jawhar et al, 1996). ACLSV was second in pxevalence, occurring in 27.1% of the surveyed orchards, followed by PDV (22.4%) and ApMV (2.1 %).…”
Field surveys were carried out in the main peach-growing areas of Lebanon to assess the presence and distribution of viruses and viroids in commercial orchards. Field inspections were made in spring and summer 2000 to observe symptoms of virus and viroid diseases respectively. In total, 950 trees in 95 commercial plantings from three different regions of Lebanon (Bekaa Valley, Mount Lebanon and north Lebanon) were surveyed and sampled. Immunoenzymatic tests (DAS-ELISA) were used to ascertain the presence of the following: Prunus necrotic ring spot ilarvirus (PNRSV), Prune dwarf ilarvirus (PDV), Apple mosaic ilarvirus (ApMV), Apple chlorotic leaf spot trichovirus (ACLSV), Plum pox potyvirus (PPV), Tomato ringspot nepovirus (ToRSV) and Strawberry latent ringspot nepovirus (SLRSV). Peach latent mosaic pelamoviroid (PLMVd) and Hop stunt hostuviroid (HSVd) were identified by molecular hybridization. About 25% of the tested samples were infected by one or more viruses. In particular, the prevailing virus was PNRSV (61.2% of infection), followed by ACLSV (27.1%), PDV (22.4%) and ApMV (2.1%). Mixed infections were about 13%. ToRSV, SLRSV and PPV were not found. HSVd was apparently absent, whereas PLMVd was identified in 34% of the samples examined. This viroid prevailed in certain areas of Mount Lebanon in both native and foreign cultivars.
“…It is comparable with that recorded from neighbouring countries, i.e. Jordan (Al‐Rwahnih et al ., 2001), Lebanon (Jawhar et al ., 1996) and Palestine (Jarrar et al ., 2001). PPV was reported earlier in Syria (Dunez, 1986), but it seems that the eradication of the infected trees carried out by local institutions was effective in controlling the disease.…”
Field surveys were carried out in the main stone fruit‐growing areas of Syria to evaluate the sanitary status of mother blocks, varietal collections and commercial orchards. The presence of virus and virus‐like diseases was checked by enzyme‐linked immunosorbent assay (ELISA), sap transmission to herbaceous hosts, testing on the woody indicators Prunus persica cv. GF 305 and Prunus serrulata cv. Kwanzan and dot‐blot hybridization tests. A total of 1337 samples was tested by ELISA (444 apricot, 283 peach, 246 cherry, 222 almond and 142 plum). The overall mean infection rate was 13%, and the percentage infection level of single species was: peach 24%, cherry 16%, almond 13.5%, apricot 6%, plum 5%. The following viruses and viroids were detected: PNRSV, PDV, ACLSV, PPV, ApMV, PLMVd and HSVd1.
“…ACLSV has been reported from sweet cherry ( P runus avium ) from China (Z hou et al. 1996), Turkey (S ipahioglu and B aloglu 2006), Lebanon (J awhar et al. 1996), Syria (I smaeil et al.…”
Section: Percent Identities Nucleotide (Above Diagonal) and Amino Acmentioning
confidence: 99%
“…Apple chlorotic leaf spot virus, a type species of the genus Trichovirus (Martelli et al 1994), frequently causes symptomless infections but can induce severe graft incompatibilities in some Prunus combinations, causing major problems in nurseries (Ulubas and Ertunc 2005). ACLSV has been reported from sweet cherry (P runus avium) from China (Zhou et al 1996), Turkey (Sipahioglu and Baloglu 2006), Lebanon (Jawhar et al 1996), Syria (Ismaeil et al 2002), Italy (Grayaa et al 1993) and Japan (Isogai et al 2004). Myrta (1998) reported 40% infection of ACLSV in sweet cherry from Albania.…”
Himalayan wild cherry (Prunus cerasoides), widely distributed in the Himalayas, was found to exhibit mild virus-like symptoms. Leaf samples from affected plants were subjected to enzyme-linked immunosorbent assay indexing for known pome and stone fruit viruses, viz., Apple mosaic virus, Apple chlorotic leaf spot virus (ACLSV), Prunus necrotic ring spot virus and Apple stem grooving virus. Results showed the presence of ACLSV. For further characterization, the coat protein gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR). Sequencing of the amplicon confirmed the presence of ACLSV. The isolate shared 98% amino acid sequence identity with P1R9D9 (Bulgarian) and C-2 (Hungarian) isolates of ACLSV. This study reports the presence of ACLSV in Himalayan wild cherry.
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