Preliminarly Survey Results and Phylogenetic Analysis for Tomato Yellow Leaf Curl Virus and Potato Leaf Roll Virus on Tomato Grown in Adana

Abstract

Surveys were conducted at tomato production sites in Adana province in 2019. 53 tomato plants have been collected showing the virus symptom due to determining its prevalence. The collected samples were tested for 13 different viral agents harmful to tomatoes using ELISA and RT-PCR methods. 24.52% TYLCV and 9.43% PLRV were detected from the tested samples. When DNA sequencing comparisons are made from RT-PCR products, 98,74% of PLRV isolates in tomato samples collected from Adana province are homogical similarity with Belgium potato isolate (KX364206.1). Also it clustered at 99.37% similar to same branch with New Zealand (GU002341.1) (BLAST at NCBI).