Preinoculation with Endophytic fungus Phomopsis liquidambaris reduced rice bakanae disease caused by Fusarium proliferatum via enhanced plant resistance

Abstract

Aims: This study evaluated the control effect of the endophytic fungus Phomopsis liquidambaris B3 against rice bakanae disease (RBD) caused by Fusarium proliferatum and the disease control result of different inoculation times of beneficial micro-organisms. Methods and Results:Rice seedlings preinoculated, coinoculated and noninoculated with B3 were exposed to F. proliferatum stress and grown under controlled conditions. Greenhouse experimental results showed that rice preinoculation with B3 significantly reduced rice bakanae disease by 21.45%, inhibited the colonization of F. proliferatum, increased defence-related enzyme activities, upregulated the expression of defence genes and promoted plant photosynthesis. However, bakanae disease in rice coinoculation with B3 increased by 11.45%, resulted in excessive reactive oxygen species (ROS) bursts and plant cell death.Conclusions: Preinoculation with the endophytic fungus P. liquidambaris B3 significantly reduced rice bakanae disease by triggering the SA-dependent defence pathways of plants, and promoted plant growth. However, coinoculatiton with P. liquidambaris B3 activated excessive defence responses, resulting in plants cell death and aggravation of bakanae disease. Significance and Impact of the Study:This study indicated that P. liquidambaris B3 was an effective method for agricultural control against rice bakanae disease caused by F. proliferatum, and provides an experimental basis for the development of sustainable endophytic fungal resources to effectively control plant diseases caused by pathogenic fungi, and suggests that precise application of beneficial microorganisms may be become a key factor in farmland crop disease management.

The colonization of soil microorganism affected the growth and the accumulation of secondary metabolites of Bletilla striata (Thunb.) Rchb. F

“…Here we demonstrate that, as for other plants of economic or medicinal interest [34][35][36], the microbial communities that colonize the roots, tubers and leaves of B. striata can influence its growth and production of secondary metabolites.…”

Endophytic Fungi in Rice Plants and Their Prospective Uses

“…They have antagonistic activity and can inhibit the incidence rate of rice brown spot disease (Bipolaris oryzae) and stimulate plant growth [35,39] (Table 1). After pre-inoculation with the endophytic fungus P. Liquidambari, the rice bakanae disease caused by Fusarium proliferatum was significantly decreased by inducing the defense responses of rice seedlings [110]. Endophytic fungi, such as Antennariella placitae, Cladosporium sp., Alternaria sp., Trichoderma sp., Sarocladium sp., Dendryphiella sp., Phoma sp., Bipolaris sp., Penicillium sp., Fusarium sp., and Aspergillus sp., exhibited significant antagonistic activity against multiple plant pathogenic fungi [38,44,111].…”

“…The multiple attributes of endophytic fungi can play a pivotal role in improving rice growth under abiotic stresses [126] (Table 1). Endophytic fungi colonization in rice plants can improve the stress resistance of rice plants, such as salt resistance and drought resistance, reduce water consumption, and enhance plant growth rate and biomass [60,[107][108][109][110][111][112][113][114]117,[126][127][128][129]. After colonization of the salt-sensitive rice variety IR-64, the salt-tolerant endophytic fungus Fusarium sp.…”

Streptomyces as a promising biological control agents for plant pathogens

“…Bakanae disease causes grain sterility, reducing yield. The illness can reduce field yield and quality by 70% ( Zhu et al, 2022 ). Ustilaginoidea virens causes false or green smut, a rice disease in India.…”