Preingestive selection of different microalgal mixtures in Crassostrea gigas and Mytilus edulis, analysed by flow cytometry

Bougrier, Serge; Hawkins, A. J. S.; Héral, Maurice

doi:10.1016/s0044-8486(96)01457-3

Cited by 124 publications

(54 citation statements)

References 22 publications

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“…Differential retention of filtered particles has been well established using flow cytometry in many shellfish species (e.g. Bougrier et al 1997, and references therein). For these reasons, it may not be acceptable to calculate FR (mg h-') indirectly as the product of CR (1 h-') and food availability measured either as TPM (mg 1-l) or POM (mg 1-l).…”

Section: Discussionmentioning

confidence: 99%

Suspension-feeding behaviour in tropical bivalve molluscs:Perna viridis, Crassostrea belcheri, Crassostrea iradelei, Saccostrea cucculata and Pinctada margarifera

Hawkins¹,

Smith²,

Tan³

et al. 1998

Mar. Ecol. Prog. Ser.

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Filter-feeding behaviours of the mussel Perna viridis and the oysters Crassostrea belchen, Crassostl-ea ~radelei, Saccostrea cucculata and Pinctada mal-garifera were compared during natural tidal vanations in the concentration (6 to 40 dry mg total particulate mass I-') and organic content (6 to 2 2 % ) of seston available in the Merbok mangrove system, Malaysia. In P. viridis, C. belcheriand S. cucculata, declining retention eff~ciencles for the largest available particle slze classes of more than 6 to 12 pm diameter were associated w t h an overall organic enrichment of filtered relative to available matter. As seston avdilab~lity increased, a ~n i n~m u m average of 71°,. of the additional filtered matter was rejected by each species as pseudofaeces prior to ingestion. And in all 5 species, preferential rejection as pseudofaeces of filtered particles with higher average inorganic content resulted in the net organic enrichment of ingested relative to filtered matter. In P. viridis and C. belcheri, the efficiency of that net organic selection declined for seston of lower organlc content. Combining all data from each species, absorption efficiencies from ingested organics increased exponent~ally with the organic content of ingested matter Collective findings suggest that key interrelations between component processes of nutrient acquisition were similar to those that have been established for the modeling of feeding, growth and environmental ~mpact among filter-feeding bivalves froin temperate latitudes However, especially fasl growth in P viridis stemmed from higher average (* 2 SE) clearance rates (13.2 A 7.0 l h-' g-' when particulate organic matter c 1 mg I-'), greater average organic enrichment of filtered relative to available matter (0.99 + 0.67) and greater average organic enrichment of ingested relative to filtered matter (0.63 * 0.03) than have hitherto been recorded in any species of bivalve filterfeeder. In contrast, P. margarifera was least well adapted for the pre-ingestive selection of organic matter, with no differential retention on the gill and little selective rejection as pseudofaeces, representing at least part of the physiological basis for characteristically slow growth in pearl oysters.

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Section: Discussionmentioning

confidence: 99%

Suspension-feeding behaviour in tropical bivalve molluscs:Perna viridis, Crassostrea belcheri, Crassostrea iradelei, Saccostrea cucculata and Pinctada margarifera

Hawkins¹,

Smith²,

Tan³

et al. 1998

Mar. Ecol. Prog. Ser.

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“…Similarly Deslous-Paoli et al (1987) Phaedactylum tricornatum retained 27.5 pg C h-' g-' demonstrated that the oyster is not able to retain small for a phytoplankton concentration of 1 X 106 cells 1-l. particles. Bougrier et al (1997) reported that the selecCiliates may thus contribute to the carbon requiretion of algae by the oyster C. gigas was independent of ments of Crassostrea gigas in the same way as do het-erotrophic flagellates for the mussels Geukensia demissa and Mytilus edulis (Kreeger & Newel1 1996).…”

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confidence: 96%

“…Indeed, the flagellate sizes in our suspensions than phytoplankton carbon content per cell from 10 to were at the lower end of the particle size spectrum 21 pg C cell-' for Skeletonema costatum (Strathmann known to be retained by Crassotrea gigas. Barille et al 1967, Burkhardt & Riebesell 1997, Bougrier et al 1997 showed that this oyster has a limited capacity to 1.61 pg C cell-' for Phaedactylum tncornatum (Fialaretain small particles: 4 pm particles (equivalent spherMedioni et al 1983) and 10.3 pg C cell-' for Navicula ical diameter, ESD) were retained with 100% retention filata (Bougrier et al 1997). In our experiment, on averefficiency when sestonic load was low, but the limlt age, oysters retained 126 pg ciliate C h-' g-' for a ciliincreased to 12 pm for hlgher sestonic loads; for partlate concentration of 25 000 t 3900 cells 1-'.…”

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confidence: 99%

Retention of ciliates and flagellates by the oyster Crassostrea gigas in French Atlantic coastal ponds:protists as a trophic link between bacterioplankton and benthic suspension-feeders

Dupuy¹,

Gall²,

Hartmann³

et al. 1999

Mar. Ecol. Prog. Ser.

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Retention of ciliates and flagellates by the oyster. In order to evaluate the importance of the 'protozoan trophic link' for energy transfer from the 'microbial food web' to large benthic suspension feeders, we offered a coastal pond comn~unity of ciliates and flagellates as potential prey to the oyster Crassostrea gigas. Clearance rate, filtered particles and relative retention efficiency were evaluated. In the grazing experiment, 94 % of ciliates and 86% of flagellates (size between 4 and 72 pm), were retained by the oyster. Whatever their size, protists were similarly retained by the oyster gills. In terms of carbon, oysters retain on average 126 pg C h-' g-' dry weight, a value over 4 times h~g h e r than reported for phytoplankton. These results indicate that a field community of protists can contribute in coastal oyster rearing ponds to the energy requirements of the oyster C. gigas. We report here the first experimental ev~dence of a significant retention of a protist community by oysters, supporting the role of protists as a trophic link between picoplankton and benthic filter-feeding bivalves. The importance of phytoplankton in the nutrition of oysters is well documented , Pastoureaud et al. 1996. However, in oyster rearing environments, such as the particularly light-limited turbid estuary of Marennes-Oleron, or in coastal ponds of the Charente where nutrients are quickly exhausted, phytoplankton cannot entirely account for the energy requirements of oysters .In the oceans, more than 50% of the primary production is due to unicellular organisms less than 3 pm in size (Li et al. 1983, Glover et al. 1986), which constitutes a nutrient source of particulate and dissolved organic matter for heterotrophic organisms. Dissolved organic matter (DOM) present in coastal waters (Pomeroy & Wiebe 1993) provides a potential for high bacterial production. Thus, in the 0 Inter-Research 1999Resale of full artrcle not perrnltted

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“…Such size-dependant asymptotic curves may be typical for many particle types, but there is persistent evidence of partially sizeindependent retention for certain particle types (Kiør-boe & Møhlenberg 1981; for review see also Ward & Shumway 2004). Reports of size-independent retention are particularly intriguing (Newell et al 1989, Bougrier et al 1997, since capture and retention are thought to be purely mechanical or hydro-mechanical processes (Jørgensen 1981, Beninger et al 1992, 2003, Nielsen et al 1993, Silverman et al 1996b, Ward et al 1998b, Beninger & Decottignies 2008.…”

Section: Discussionmentioning

confidence: 99%

In situ evidence for pre-capture qualitative selection in the tropical bivalve Lithophaga simplex

Yahel¹,

Marie²,

Beninger³

et al. 2009

Aquat. Biol.

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Few feeding studies have been performed on tropical bivalves, and in situ feeding studies are lacking altogether. We investigated retention efficiencies for natural particles in the coralboring tropical mytilid Lithophaga simplex. Using the in situ InEx technique (Yahel et al. 2005; Limnol Oceanogr Methods 3:46-58) SCUBA divers collected samples from the water inhaled and exhaled by undisturbed bivalves at the coral reef of Eilat (Gulf of Aqaba). Particle retention efficiencies were determined using flow cytometry analysis of the paired water samples. The photosynthetic bacterium Synechococcus (0.9 ± 0.1 μm) and larger eukaryotic algae (1 to 10 μm) were preferentially retained by the bivalve with removal efficiencies of up to 90% (1996 to 2000: averages of 69 ± 14% and 60 ± 17%, respectively, n = 74 individual bivalves). The minute photosynthetic bacterium Prochlorococcus (0.4 ± 0.1 μm) was also moderately retained (41 ± 19%). Only a small proportion of the non-photosynthetic bacteria (0.3 ± 0.1 μm) were retained (5 ± 18%, median of 9%), despite their numerical dominance in the plankton and considerable size overlap with Prochlorococcus. Sizeindependent preferential retention was also observed within particle types: (1) L. simplex more efficiently retained Prochlorococcus and picoeukaryotic algal cells with higher chlorophyll content and (2) the small fraction of non-photosynthetic bacteria retained did not differ in size, but had higher nucleic acid content (compared to the inhaled population) an indicator for viable and active bacteria. We conclude that particle retention is not strictly size-dependent in L. simplex, and probably involves other cell attributes such as cell surface properties and/or motility.

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Preingestive selection of different microalgal mixtures in Crassostrea gigas and Mytilus edulis, analysed by flow cytometry

Cited by 124 publications

References 22 publications

Suspension-feeding behaviour in tropical bivalve molluscs:Perna viridis, Crassostrea belcheri, Crassostrea iradelei, Saccostrea cucculata and Pinctada margarifera

Suspension-feeding behaviour in tropical bivalve molluscs:Perna viridis, Crassostrea belcheri, Crassostrea iradelei, Saccostrea cucculata and Pinctada margarifera

Retention of ciliates and flagellates by the oyster Crassostrea gigas in French Atlantic coastal ponds:protists as a trophic link between bacterioplankton and benthic suspension-feeders

In situ evidence for pre-capture qualitative selection in the tropical bivalve Lithophaga simplex

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