Preimplantation genetic testing

Chamayou, Sandrine

doi:10.1016/b978-0-323-89907-9.00011-9

Cited by 1 publication

(1 citation statement)

References 149 publications

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“…Since the first clinical applications in 1992, the proportion of ICSI on conventional IVF did not stop to increase, getting stabilized at 70% of the fresh in vitro cycles since 2013 [ 4 ]. ICSI is the in vitro fertilization method of choice when using frozen-thawed oocytes [ 5 ] and surgically retrieved spermatozoa [ 6 ], as well as in case of preimplantation genetic testing (PGT) [ 7 ]. ICSI can be performed with frozen-thawed spermatozoa [ 8 ] and with immature spermatozoa [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

What Does Intracytoplasmic Sperm Injection Change in Embryonic Development? The Spermatozoon Contribution

Chamayou

Giacone

Cannarella

et al. 2023

JCM

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The intracytoplasmic sperm injection (ICSI) technique was invented to solve severe male infertility due to altered sperm parameters. Nowadays, it is applied worldwide for the treatment of couple infertility. ICSI is performed with any available spermatozoon from surgery or ejaculated samples, whatever are the sperm motility, morphology or quantity. The aim of the present review was to study if embryo development and kinetics would be modified by (1) ICSI under the technical aspects, (2) the micro-injected spermatozoa in connection with male infertility. From published data, it can be seen that ICSI anticipates the zygote kinetics Furthermore, because fertilization rate is higher in ICSI compared to conventional in vitro fertilization (IVF), more blastocysts are obtained for clinical use in ICSI. Sperm and spermatozoa characteristics, such as sperm parameters, morphology and vitality, DNA content (levels of sperm DNA fragmentation, microdeletions, and chromosomal abnormalities), RNA content, epigenetics, and sperm recovery site (testicular, epididymis, and ejaculated), have an impact on fertilization and blastocyst rates and embryo kinetics in different ways. Even though ICSI is the most common solution to solve couples’ infertility, the causes of male infertility are crucial in building a competent spermatozoa that will contribute to normal embryonic development and healthy offspring.

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