Preharvest Hydrogen Peroxide Treatment Delays Leaf Senescence of Chinese Flowering Cabbage During Storage by Reducing Water Loss and Activating Antioxidant Defense System

Abstract

Leaf yellowing, an indicator of senescence, reduces commercial value of Chinese flowering cabbage after harvest. Hydrogen peroxide (H2O2) plays a dual role in mediating plant stress responses, but it is not clear whether and how it affects leaf senescence when exogenously stimulating the plants before harvest. Here, we found that preharvest application with low concentrations of H2O2 to root delays leaf senescence. Around 10 mM H2O2 reduced leaf yellowing rate by 8.2 and 26.4% relative to the control following 4 and 8 days storage, respectively. The H2O2-treated cabbages showed higher chlorophyll and lower relative expression of senescence-associated gene (SAG) BrSAG12 than the control. Proteomic analysis revealed 118 and 204 differentially expressed proteins (DEPs) in H2O2-treated plants at 4 and 8 days of storage, respectively. The main DEPs are involved in chlorophyll degradation and synthesis, water deprivation, antioxidant activity, and protections on chloroplast membranes. Decline of water loss in H2O2-treated cabbages was coincide with increase of proline contents and modulation of leaf stomatal aperture. Alleviation of oxidative stress was indicated by suppression of respiratory burst oxidase homolog and upregulation of reactive oxygen species (ROS) scavenging-related genes. These results were also supported by the alleviation of lipid peroxidation and the protections on cell integrity and photochemical efficiency in H2O2-treated group. Collectively, preharvest H2O2 treatment alleviates water loss and activates antioxidant defense system, protects chloroplast membrane from oxidative damage, and ultimately delays leaf senescence during storage. This study provides novel insights into the roles of H2O2 for regulating leaf senescence of Chinese flowering cabbage.

6-Benzyladenine Treatment Maintains Storage Quality of Chinese Flowering Cabbage by Inhibiting Chlorophyll Degradation and Enhancing Antioxidant Capacity

“…Leaves of Chinese flowering cabbage are prone to senescence after harvest, and senescence-associated gene 12 ( SAG12 ) is a critical gene promoting senescence progress [ 3 ]. The results here showed that BrSAG12 in the 6-BA treatment group was inhibited compared to that in the control ( Figure 2 A).…”

“…The quality of Chinese flowering cabbage deteriorates rapidly after harvesting, which affects its commodity value and shelf life [ 3 ]. Green color and water retention of leaves are important commercial qualities of green leafy vegetables [ 19 ].…”

“…Changes in these parameters are often used as indicators to evaluate the quality deterioration and oxidative damage of plant tissues. A previous study showed that ROS and MDA levels in the leaves of Chinese flowering cabbage gradually increase over time during senescence [ 3 ]. In this study, 6-BA treatment at 10, 20, 40, and 80 mg·L −1 significantly delayed the decline in soluble proteins in the leaves of Chinese flowering cabbage.…”

“…It is popular because of its favorable taste and richness of nutrients, including vitamin C, carotenoids, dietary fiber, minerals, glucosinolates, phenolic acids, and flavonoids [ 2 ]. However, the storage and transportation of Chinese flowering cabbage are restricted because of rapid leaf yellowing, wilting, and nutrient loss after harvest [ 3 ]. The average postharvest loss of Chinese flowering cabbage is 20–40% in the absence of preservation treatment [ 4 ].…”

“…Our previous study found that preharvest treatment with selenium and SNP can effectively reduce leaf yellowing in Chinese flowering cabbage [ 2 ]. Oxidative damage induced by endogenous reactive oxygen species (ROS) was shown to be one of the main triggers of leaf senescence [ 3 ]. Over-accumulation of ROS may cause oxidative damage to proteins, DNA, and lipids in plant tissues [ 7 ].…”