Pregnancy-specific ?1-glycoprotein: cDNA cloning, tissue expression, and species specificity of one member of the PS?G family

Abstract

Pregnancy-specific beta 1-glycoprotein (PS beta G) is a heterogeneous product of the human syncytiotrophoblast, closely related to the CEA-NCA multigene family. In the present study, immunoscreening was carried out with anti-PS beta G antibodies to isolate cDNA sequences from a placental lambda gt11 expression library. One 1847-bp cDNA clone comprising the major portion of the coding sequence of a putative 48-kd peptide was sequenced and characterized. Hybridization of human genomic DNA to the PS beta G sequence revealed a complex pattern of restriction fragments, a finding well in agreement with the assumption that there are several independent PS beta G genes. A variable PstI band was found in human DNA. Transfer blot analysis of human placental RNA identified transcript of 2.2kb and 1.7kb that appear transiently with increasing levels of expression during gestation. No hybridization of PS beta G cDNA to human RNA from liver, kidney, heart, thyroid, and ovary was observed. In analyses of placental RNA from mouse, goat, sheep, and cow, no corresponding transcripts could be detected, and DNA hybridization under low-stringency hybridization conditions resulted in very faint cross-reacting bands, presumably indicating sequences that were scarcely related. However, PS beta G-specific DNA sequencies with similar restriction patterns were found in primates. These results are compatible with the assumption of late evolutionary development of certain PS beta G sequences.

Carcinoembryonic antigen gene family: Molecular biology and clinical perspectives

“…Many studies to determine expression of CEA-related genes at the transcriptional level have applied the Northern blot technique( 10, 44,54,57,58,61,65,[95][96][97][98][99][100][101][102][103][104][105][106][107]. Using this approach, and with the help of gene-specific or subgroup-specific DNA probes, a number of mRNA species varying in size have been identified in different tumors and some normal tissues.…”

Linkage of two human pregnancy-specific beta 1-glycoprotein genes: one is associated with hydatidiform mole.

“…We found that placental PS/3G represents a family of closely related glycoproteins of 72, 64, and 54 kDa and that placental poly(A)+ RNA directed the synthesis of three polypeptides of 50, 48 (major), and 36 kDa as immunoprecipitated by anti-PS3G serum (11). Moreover, near-full-length cDNAs encoding members ofthe PSf3G family were isolated and characterized by our laboratory (11,12) as well as by others (13)(14)(15)(16)(17)(18)(19)(20). Two gene fragments that share strong sequence similarity with the reported PSfG cDNAs have been reported (21,22).…”

Regional localization of the gene encoding pregnancy specific beta-1-glycoprotein 1 (PSBG1) to human chromosome 19q13.1

“…Our PSBG! cDNA (Niemann et al, 1989) is nearly 100% homologous to PSBG sequences described previously (Streydio ct al., 1988; Watanabe and Chou. 1988) and contains a segment that is identical to the clone hPS3 published by …”