Pregnancy resulting from intracytoplasmic injection of cryopreserved spermatozoa recovered from testicular biopsy

Podsiadly, Barbara; Woolcott, Robert; Stanger, James; Stevenson, Karen

doi:10.1093/oxfordjournals.humrep.a019376

Cited by 54 publications

(20 citation statements)

References 10 publications

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“…Hence, there is a need for special techniques for storing a small number of sperm. Moreover, sperm recovery for ICSI can be difficult if the sample is diluted in a large volume of cryoprotectant as is done in conventional freezing techniques [Podsiadly et al 1996].…”

Section: Storage Methods For Small Samplesmentioning

confidence: 99%

Effect of sperm storage and selection techniques on sperm parameters

Sharma

Kattoor

Ghulmiyyah

et al. 2014

Systems Biology in Reproductive Medicine

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Sperm cryopreservation preserves the fertility of cancer patients undergoing chemotherapy, ensures sperm are available at the time of oocyte retrieval in assisted reproductive technology (ART) procedures and avoids the need for repeated sperm extraction surgeries in azoospermic patients. Conventional methods of cryopreservation involve storage in liquid nitrogen (LN 2 ), which causes a significant decline in sperm parameters such as motility and viability and results in DNA damage. Newer methods of sperm cryopreservation such as the LN 2 vapor method, vitrification, and experimental methods such as lyophilization, have significant advantages over the conventional methods in terms of cost effectiveness and ease of use. Density gradient centrifugation (DGC), swim up, and magnetic assisted cell sorting (MACS) can be used prior to or post-cryopreservation to improve post-thaw sperm quality. Cryopreservation in special carriers such as cryoloops and empty zona prevents the loss of small numbers of sperm during cryopreservation. This article will discuss these sperm preservation and selection techniques.

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Section: Storage Methods For Small Samplesmentioning

confidence: 99%

Effect of sperm storage and selection techniques on sperm parameters

Sharma

Kattoor

Ghulmiyyah

et al. 2014

Systems Biology in Reproductive Medicine

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“…Formal scrotal exploration was carried out before surgical extraction. The techniques followed those described in previous reports (17). The collected testicular samples also were stored in liquid nitrogen immediately for RNA extraction and sent for pathologic examination.…”

Section: Materials and Methods Patients And Tissue Collectionmentioning

confidence: 99%

Expression of androgen receptor co-regulators in the testes of men with azoospermia

Lan

Hseh

et al. 2008

Fertility and Sterility

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“…Cryopreservation of epididymal spermatozoa and testicular tissue before ICSI treatment is efficacious and may overcome many of the problems inherent to the use of fresh testicular tissue [16][17][18][19]. Additionally, the efficacy of cryopreserving testicular spermatozoa on the day of diagnostic testicular biopsy and the lack of significant differences in terms of fertilization and embryo developmental rates compared with fresh testicular spermatozoa have been reported [12].…”

Section: Discussionmentioning

confidence: 99%

“…Clinical pregnancies and delivery of healthy babies have been achieved with surgically retrieved epididymal [1][2][3][4] and testicular spermatozoa [5][6][7][8], but one of the major drawbacks lies in the need to 16 Urol Int 2000;65: [15][16][17][18][19][20] Trombetta/Liguori/Gianaroli/Magli/ Selman/Colpi/Belgrano/Vitali/Ferraretti resort to microsurgery at each ICSI attempt. Besides the patient's discomfort, repeated operations can damage epididymal and testicular tissues and, indeed, percutaneous epididymal sperm aspiration and testicular sperm aspiration were developed to recover sperm cells from the epididymis and testis without repeated surgical interventions [9,10].…”

Section: Introductionmentioning

confidence: 99%

Testicular Sperm Extraction Combined with Cryopreservation of Testicular Tissue in the Treatment of Azoospermia

Trombetta

Liguori²,

Gianaroli³

et al. 2000

Urol Int

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Background: The aim of the present study was to verify the feasibility of cryopreserving testicular tissue during the first diagnostic biopsy and then using thawed sperm to inseminate the partner’s oocytes. The expected advantages are: (i) minimal risk of not having spermatozoa available at the time of intracytoplasmic sperm injection; (ii) no repeated surgical interventions, and (iii) programming the treatment cycle at the couple’s convenience. Materials and Methods: Between May 1996 and May 1998, 64 azoospermic patients underwent investigative testicular biopsy combined with cryopreservation of spermatozoa which were retrieved in a simultaneously examined fresh sample. Testicular tissue cryopreservation was carried out in 43 cases (67%) for later intracytoplasmic sperm injection attempts. Results: In all, 23 couples underwent 26 assisted conception cycles; the fertilization rate was 64% with spermatozoa (139/218, 24 cycles), 40% with round spermatids (2/5, 1 cycle), and 69% with elongated spermatids (9/13, 1 cycle). The embryo cleavage rate was 84%. The mean number of embryos replaced in 24 patients was 2.7 ± 0.7. In 2 cases, embryo quality was very poor, and they were not transferred to the patients. Eight clinical pregnancies resulted (35%/patient and 33%/transferred cycle) with an implantation rate of 14.1%; 2 patients have already delivered and 6 pregnancies are ongoing normally. Conclusions: Testicular tissue cryopreservation during the first diagnostic biopsy is an alternative to repeated surgical interventions. Patients can initiate an ovarian stimulation cycle, confident of having spermatozoa available. Moreover, since only one straw is routinely used for each intracytoplasmic sperm injection cycle, the frozen tissue remains as a sperm source for multiple attempts.

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Pregnancy resulting from intracytoplasmic injection of cryopreserved spermatozoa recovered from testicular biopsy

Cited by 54 publications

References 10 publications

Effect of sperm storage and selection techniques on sperm parameters

Effect of sperm storage and selection techniques on sperm parameters

Expression of androgen receptor co-regulators in the testes of men with azoospermia

Testicular Sperm Extraction Combined with Cryopreservation of Testicular Tissue in the Treatment of Azoospermia

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