Pregnancy rates relative to recipient plasma progesterone levels on the day of nonsurgical transfer of frozen/thawed bovine embryos

Niemann, Heinrich; Sacher, Bernhard; Elsaesser, Folkmar

doi:10.1016/0093-691x(85)90197-9

Cited by 46 publications

(31 citation statements)

References 15 publications

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“…Following years of a prevalent belief that the higher values of P4 are better [5], it now seems that there may be an optimal window for P4 values during the luteal phase in bovine IVF [10]. Though progesterone levels on the day of transfer have not yet been studied extensively in humans, timing the transfer of a frozen embryo based on serial P4 values rather than cycle day number alone results in higher pregnancy rates [11].…”

Section: Introductionmentioning

confidence: 99%

Serum progesterone levels greater than 20 ng/dl on day of embryo transfer are associated with lower live birth and higher pregnancy loss rates

Kofinas

Blakemore

McCulloh

et al. 2015

J Assist Reprod Genet

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Background Progesterone (P4) is essential for support of the endometrium and implantation of an embryo in the normal menstrual cycle. In programed frozen embryo transfer cycles using exogenous P4 is necessary, as the endogenous production of P4 requires a functioning corpus luteum that is not present in programed cycles. To date, there is continuing debate about ideal serum estradiol and P4 values in frozen embryo transfer cycles. Methods Patients underwent single euploid embryo frozen transfer cycles from 2010 to 2013 at a single large academic center. Patients using donor oocytes and patients with changes in progesterone dose during the cycles in question were excluded. All cycles were programed and intramuscular P4 was used exclusively. Only patients administering the same daily dose of P4 throughout the cycle were included (N=213 patients). Main outcomes were ongoing pregnancy/live birth rates (OPR/LBR), clinical pregnancy rates (CPR), and spontaneous abortions/biochemical pregnancies. CPR was defined by the presence of a sac on 1st trimester ultrasound. Missed abortions were calculated per pregnancy with a sac. Receiver operator characteristic curves (ROC curves) and chi-squared tests were performed for statistical analysis. Results Two groups based on day 19 P4 levels were compared (group A, P4<20 ng/ml; group B, P4>20 ng/ml). OPR/LBRs were 65 vs. 49 %, group A vs. B, p value=0.02, RR=1.33 (1.1-1.7). Missed abortion and biochemical rates were higher in group B as opposed to group A, 27 vs. 12 %, p=0.01, RR=0.45(0.24-0.86). When P4 was stratified into five groups based on nanogram per milliliter of progesterone on day 19 (10-15, 15-20, 20-30, 30-40, and >40), there was a trend downward in OPR/LBR (70, 62, 52, 50, and 33 %, respectively). There was also an increase in missed abortion/biochemical rates (7, 15, 27, 32, and 20 %, respectively). Multiple logistic regression showed an increase in OPR/LBR when accounting for age, day 2 FSH, weight, number of embryos biopsied, and number of euploid embryos. Conclusion P4 levels >20 ng/ml on the day of transfer (during frozen single euploid embryo transfer cycles) were associated with decreased OPR/LBR.

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Section: Introductionmentioning

confidence: 99%

Serum progesterone levels greater than 20 ng/dl on day of embryo transfer are associated with lower live birth and higher pregnancy loss rates

Kofinas

Blakemore

McCulloh

et al. 2015

J Assist Reprod Genet

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“…Por otro lado, estudios en bovinos coinciden en señalar que la medición del CL y la concentración de progesterona plasmática de las receptoras al momento de la transferencia embrionaria están relacionadas positivamente, aunque sin observar diferencias significativas en preñez post transferencia embrionaria (Niemann et al, 1985;Spell et al, 2001;Moreno et al, 2003).…”

Section: Resultados Y Discusiónunclassified

Transferencia Embrionaria Ipsilateral Y Contralateral a La Posición Del Cuerpo Lúteo Y Supervivencia Embrionaria en Llamas.

Cordero

et al. 2011

Rev. investig. vet. Perú

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RESUMENEl presente trabajo se realizó con el propósito de evaluar el efecto de la transferencia embrionaria ipsilateral y contralateral a la posición del cuerpo lúteo (CL), así como el tamaño del CL sobre la tasa de supervivencia embrionaria en llamas. Se utilizaron 43 llamas receptoras, adultas, distribuidas aleatoriamente en 4 grupos: G1 (n=10): CL en ovario derecho y transferencia ipsilateral, G2 (n=10): CL en ovario derecho y transferencia contralateral, G3 (n=15): CL en ovario izquierdo y transferencia ipsilateral y G4 (n=8): CL en ovario izquierdo y transferencia contralateral. Se usaron 10 llamas como donadoras de embriones, que fueron sincronizadas con LH (1 ml) (Día 0=D0), superovuladas con 1000 UI de eCG en el D3, a las que se les provocó luteólisis con PGF 2? en el D7 y se les empadró en el D8. Ese día, las receptoras se trataron con LH para sincronizarlas con las donadoras. En el D14 se colectó, evaluó y transfirió los embriones. El 60 (G1) y 75% (G3) de las hembras preñaron con transferencia ipsilateral derecha e izquierda, respectivamente, mientras solo el 30 (G2) y 25% (G4) preñaron con transferencia contralateral derecha e izquierda, respectivamente. Sin embargo, solo se encontró diferencia significativa entre el grupo G3 con los grupos G2 y G4 (p<0.05). Los resultados indican una mayor tasa de supervivencia embrionaria en llamas al realizar la transferencia en el cuerno ipsilateral a la posición del CL ubicado en el ovario izquierdo.

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“…According to previous reports [8,13,14], they were classified as favorably developing CL, poorly developing CL, and cystic CL. A favorably developing CL has a major axis of 1.5 cm or more and a clear crown, a poorly developing CL has a major axis of less than 1.5 cm and a clear crown, and a cystic CL is associated with palpable retention of luminal fluid and an obscure crown.…”

Section: Examination and Classification Of Corpus Luteummentioning

confidence: 99%

“…In embryo transfer, if blood and milk concentrations are determined in candidate recipient cows before transfer and the optimal recipient cows can be selected, the embryo transfer performed under such conditions should lead to high pregnancy rates. The correlation between the P concentrations and the rate of pregnancy have been investigated by determining blood P concentrations on day 6 [7] and day 7 [8][9][10][11] in bovine recipients , but no positive relationship between them has as yet been revealed. With the direct transfer and the one-step straw methods, there is the possibility that degenerated embryos will be transferred, because these methods allow no confirmation of the survivability or quality of post-thawing embryo [4].…”

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confidence: 99%

Pregnancy Rate and Blood Progesterone Concentrations on the Previous Day and the Day of Frozen Embryo Transfer in Parous Recipient Cows of Japanese Black.

Nishigai

Kamomae

Tanaka

et al. 1998

Journal of Reproduction and Development

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Abstract. Blood progesterone (P) concentrations were determined on the previous day (6 days after onset of estrus; day 6) and the day of embryo transfer (7 days after onset of estrus; day 7) in recipients of Japanese Black beef cow to clarify the correlation between luteal function and pregnancy rate in the stepwise transfer method of frozen Japanese Black embryos. When the blood P concentrations on both day 6 and day 7 in the bovine recipients increased, the pregnancy rate also tended to increase. Pregnancy rates in the recipients with blood P concentration <2.5 ng/ml and 2.5 ng/ml on day 6 were 38.6% (17/44) and 60.7% (34/56), respectively, showing a significant (P <0.05) difference. The pregnancy rate in the recipients with blood P concentration <2.5 ng/ml on day 7 was 35.7% (10/28), while the corresponding rate was 56.9% (41/72) in the recipients with blood P concentration of 2.5 ng/ml, showing no significant difference. The pregnancy rate in the recipients in which blood P concentration was <2.5 ng/ml on day 6, and increased to 2.5 ng/ml on day 7 was low at 45.0% (9/20), while the rate in the recipients which showed blood P concentration of 2.5 ng/ml on both day 6 and day 7 was as high as 61.5% (32/52). The relationship between the blood P concentration and luteal development on day 6 corresponding to pregnancy rate was investigated by dividing corpus luteum (CL) into the following three groups by rectal palpation findings : favorably developing, poorly developing and cystic groups. The mean blood P concentration was 2.8 ng/ml in the favorably developing CL, whereas the levels were as low as 2.0 ng/ml and 2.1 ng/ml in the poorly developing CL and cystic CL, respectively. The pregnancy rate for the favorably developing CL was 55.3% (42/76) which tended to be higher than for the poorly developing CL , (44.4% (8/18)), and cystic CL, (16.7% (1/6)), respectively. These observations showed that a high pregnancy rate is achieved by selecting cows with blood P concentration of 2.5 ng/ml on day 6 and day 7 of embryo transfer.

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Pregnancy rates relative to recipient plasma progesterone levels on the day of nonsurgical transfer of frozen/thawed bovine embryos

Cited by 46 publications

References 15 publications

Serum progesterone levels greater than 20 ng/dl on day of embryo transfer are associated with lower live birth and higher pregnancy loss rates

Serum progesterone levels greater than 20 ng/dl on day of embryo transfer are associated with lower live birth and higher pregnancy loss rates

Transferencia Embrionaria Ipsilateral Y Contralateral a La Posición Del Cuerpo Lúteo Y Supervivencia Embrionaria en Llamas.

Pregnancy Rate and Blood Progesterone Concentrations on the Previous Day and the Day of Frozen Embryo Transfer in Parous Recipient Cows of Japanese Black.

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