Pregnancy rates of beef cattle are not affected by<i>Campylobacter fetus</i>subsp.<i>venerealis</i>real-time PCR-positive breeding sires in New Zealand

Sanhueza, Juan; Heuer, C; Jackson, Rodney; Hughes, Paul E.; Anderson, Peter T.; Kelly, Kristen; Walker, Greg

doi:10.1080/00480169.2014.898202

Cited by 9 publications

(10 citation statements)

References 17 publications

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“…C. fetus subspecies fetus and C. fetus subspecies venerealis infections are causes of reproductive losses in Australia ( 49 ). In New Zealand, disease was last reported in 1993 ( 123 ) but is now considered absent ( 52 ). The PCR test used to detect the presence of C. fetus subspecies venerealis in New Zealand was shown to be cross-reactive with C. hyointestinalis ( 124 ).…”

Section: Abortifacients In Australia and New Zealandmentioning

confidence: 99%

Review of Diagnostic Procedures and Approaches to Infectious Causes of Reproductive Failures of Cattle in Australia and New Zealand

2018

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Infectious causes of reproductive failure in cattle are important in Australia and New Zealand, where strict biosecurity protocols are in place to prevent the introduction and spread of new diseases. Neospora caninum ranks highly as an important cause of reproductive wastage along with fungal and bacterial infections. Brucella, a leading cause of abortion elsewhere in the world, is foreign, following successful programs to control and eradicate the disease. Leptospirosis in cattle is largely controlled by vaccination, while Campylobacter and Tritrichomonas infections occur at low rates. In both countries, Bovine Viral Diarrhea virus (BVDV) infection rates as the second most economically important disease of cattle and one that also has an effect on reproduction. Effective disease control strategies require rapid diagnoses at diagnostic laboratories. To facilitate this process, this review will discuss the infectious causes of reproductive losses present in both countries, their clinical presentation and an effective pathway to a diagnosis.

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Section: Abortifacients In Australia and New Zealandmentioning

confidence: 99%

Review of Diagnostic Procedures and Approaches to Infectious Causes of Reproductive Failures of Cattle in Australia and New Zealand

2018

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“…Although the parA gene is still used for Cfv identification, the horizontal gene transfer of parA to C. hyointestinalis isolates and Cff strains was reported [ 22 , 23 ]. Also, parA detection assays identified putative positive Cfv bulls in herds without impairment of the reproductive performance [ 24 ] and in virgin bulls [ 17 , 25 ]. Indeed, the parA gene can be found in mobile genetic elements, namely in a GI almost exclusive of Cfv [ 26 , 27 ], in the chromosome and in extra-chromosomal plasmids [ 21 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment of Campylobacter fetus subsp. venerealis molecular diagnosis using clinical samples of bulls

et al. 2020

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Background Campylobacter fetus subsp. venerealis (Cfv) is the pathogen responsible for Bovine Genital Campylobacteriosis (BGC), a venereal disease of cattle associated with impaired reproductive performance. Although several PCR assays were developed to identify this pathogen, most of them are still poorly evaluated in clinical samples. This study evaluated real-time PCR assays for Cfv detection in preputial samples of bulls (n = 308). Results The detection at the subspecies level (Cfv) compared four assays: two targeting ISCfe1 and two targeting parA gene. The detection at the species level (C. fetus) considered an assay targeting the nahE gene and a commercial kit for C. fetus identification. At the subspecies level, assays directed either to different targets (parA and ISCfe1), or to the same target (ISCfe1 or parA), showed a high percentage of disagreeing results. All samples positive at the subspecies level (n = 169) were negative in C. fetus detection assays, which strongly suggests the horizontal gene transfer of ISCfe1 and parA to other bacterial species. This was confirmed by microbiological isolation of three Campylobacter portucalensis strains responsible for false positive results. Sequences with a high level of identity with ISCfe1 and parA gene of Cfv were identified in C. portucalensis genome. Conclusions Overall, this study reveals that PCR assays solely directed to a subspecies target originate a high rate of false positive results, due to the presence of parA and ISCfe1 homologous sequences in other bacterial species, namely of the genus Campylobacter. Although the specificity of these methods may be higher if applied to bulls from herds with clinical features of BGC or in other geographical regions, current PCR diagnosis should couple subspecies and species targets, and further research must be envisaged to identify Cfv specific molecular targets.

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“…Campylobacteriosis is a disease of socio-economic and public health significance and is a list B notifiable disease of the World Organization of Animal Health (OIE) [6]. Campylobacteriosis exists worldwide including Latin-America, Africa, Asia, North America, Europe, and NewZealand [6][7][8][9][10]. The prevelance of CFv in some countries is still questionable due to lack of reporting and reliable testing [9,10].…”

Section: Reviewmentioning

confidence: 99%

“…Campylobacteriosis exists worldwide including Latin-America, Africa, Asia, North America, Europe, and NewZealand [6][7][8][9][10]. The prevelance of CFv in some countries is still questionable due to lack of reporting and reliable testing [9,10]. Bovine infections with C. fetus subsp.…”

Section: Reviewmentioning

confidence: 99%

“…Utilization of PCR for diagnosis has provided a way to easily differentiate between subspecies. However, not all PCRs are alike and so there have been mixed results [9][10][11][12]. The development of a new real-time quantitative polymerase chain reaction (qPCR) test has shown to be very reliable and was used to diagnose CFv infection in a Canadian cattle herd [11].…”

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confidence: 99%

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Bovine Genital Campylobacteriosis - A Review

Gard¹

2016

Int J Vet Sci Res

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Bovine genital campylobacteriosis (BGC) is a true venereal disease of cattle. This disease results in infertility, embryonic and fetal death, metritis, salpingitis, pyometra, and abortion in cattle, and sheep and goats as well. The causal agent of BGC, is Campylobacter fetus. This organism is found worldwide and causes significant economic losses in endemic areas where appropriate management has not been instituted.

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Pregnancy rates of beef cattle are not affected byCampylobacter fetussubsp.venerealisreal-time PCR-positive breeding sires in New Zealand

Cited by 9 publications

References 17 publications

Review of Diagnostic Procedures and Approaches to Infectious Causes of Reproductive Failures of Cattle in Australia and New Zealand

Review of Diagnostic Procedures and Approaches to Infectious Causes of Reproductive Failures of Cattle in Australia and New Zealand

Assessment of Campylobacter fetus subsp. venerealis molecular diagnosis using clinical samples of bulls

Bovine Genital Campylobacteriosis - A Review

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