Pregnancy rates following timed embryo transfer with fresh or vitrified in vitro produced embryos in lactating dairy cows under heat stress conditions

Al-Katanani, Y.M.; Drost, Maarten R.; Monson, R.L.; Rutledge, J. J.; Krininger, C.E.; Block, J.; Thatcher, W.W.; Hansen, Peter J.

doi:10.1016/s0093-691x(02)00916-0

Cited by 72 publications

(71 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, IVEP from slaughterhouse material provides a high number of oocytes or embryos (after in vitro maturation and fertilisation) at a low cost to carry out other associated embryo technologies such as cryobiology. In bovine, IVEP is especially interesting to mitigate the decrease in fertility suffered by dairy cattle during environmental heat stress conditions (Ambrose et al, 1999; -E-mail: miguel.silvestre@uv.es Al-Katanani et al, 2002) or to take advantage of the ovum pick-up technique (OPU). In the latter case, a small number of oocytes per female are usually recovered after OPU (approximately three to six oocytes recovered per nonstimulated cow and session; Rizos et al, 2005;Chaubal et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

View full text Add to dashboard Cite

When embryos are cultured individually or in small groups, blastocyst yield efficiency and quality are usually reduced. The aim of this work was to investigate the effect of supplementation of the embryo culture medium (CM) with several growth factors (GFs) on embryo development and apoptosis rate when a reduced number of embryos were in vitro cultured. Two experimental studies (ES) were carried out. In ES 1, five treatments were tested to study the effect of GF on embryo development: Control (,30 to 50 embryos cultured in 500 ml of CM); Control 5 (Five embryos cultured in 50 ml microdrops of CM), without addition of GF in either of the two control groups; epidermal GF (EGF); IGF-I; and transforming GF-a (TGF-a) (Five embryos were cultured in 50 ml microdrops of CM with 10 ng/ml EGF, 10 ng/ml IGF-I or 10 ng/ml TGF-a, respectively). In ES 2, following the results obtained in ES 1, four different treatments were tested to study their effect on embryo development and quality (number of cells per blastocyst and apoptotic rate): Control; Control 5; EGF, all three similar to ES 1; EGF 1 IGF-I group (five embryos cultured in 50 ml microdrops of CM with 10 ng/ml EGF and 10 ng/ml IGF-I). In both ESs, it was observed that a higher proportion of embryos cultured in larger groups achieved blastocyst stage than embryos cultured in reduced groups (22.6% v. 14.0%, 12.6% and 5.3% for Control v. Control 5, IGF-I, TGF-a groups in ES 1, and 24.9% v. 17.1% and 19.0% for Control v. Control 5 and EGF in ES 2, respectively; P , 0.05), with the exception of embryos cultured in medium supplemented with EGF (18.5%) or with EGF 1 IGF-I (23.5%), in ES 1 and ES 2, respectively. With regard to blastocyst quality, embryos cultured in reduced groups and supplemented with EGF, alone or combined with IGF-I, presented lower apoptosis rates than embryos cultured in reduced groups without GF supplementation (11.6% and 10.5% v. 21.9% for EGF, EGF 1 IGF-I and Control 5 groups, respectively; P , 0.05). The experimental group did not affect the total number of cells per blastocyst. In conclusion, this study showed that supplementation of the CM with EGF and IGF could partially avoid the deleterious effect of in vitro culture of small groups of bovine embryos, increasing blastocyst rates and decreasing apoptosis rates of these blastocysts.Keywords: bovine embryo, reduced groups, in vitro culture, growth factor, apoptosis ImplicationsIn bovine, in vitro embryo production (IVEP) is especially interesting when applying the ovum pick-up technique (OPU). However, a small number of oocytes per female are usually recovered after OPU, and when embryos are cultured individually or in a reduced group blastocyst yield efficiency and quality are generally reduced. The improvement of IVEP efficiency and quality with low numbers of embryos would be of great interest for applying this technique in the dairy industry. IntroductionIn vitro embryo production (IVEP) has numerous applications in various scientific areas such as animal production or biomedicine. IVEP-rela...

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

View full text Add to dashboard Cite

show abstract

“…Development and prevalence of estrus and ovulation synchronization for the artificial insemination and embryo transfer Accepted for publication: December 26, 2003 Correspondence: N. Kawate (e-mail: nkawate@vet.osakafu-u.ac.jp) of cows increase the opportunity of using the hormonal drugs [15][16][17]. The efficaciousness of the t r e a t m e n t w i t h G n R H a n d go n ad o t r o pi n s possessing LH activity depends partly on the quality and quantity of LH receptor in the ovary.…”

mentioning

confidence: 99%

Studies on the Regulation of Expression of Luteinizing Hormone Receptor in the Ovary and the Mechanism of Follicular Cyst Formation in Ruminants

Kawate

2004

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. In the series of studies, changes of expression and regulation of luteinizing hormone (LH) receptor in the ovary of domestic ruminants were examined. Furthermore, mechanisms of formation of follicular cysts in domestic ruminants, caused by stress and so on, were endocrinologically elucidated. Results of the studies provide the following conclusions. (1) The quantity of LH receptor in the bovine antral follicles increases rapidly in the latter stage of its development. (2) The quantity of LH receptor and its mRNA in the bovine and caprine corpus luteum increase during their developments. The increase of the receptor in the caprine luteal development is regulated by LH through the receptor mRNA level. (3) At least, three splice variants of LH receptor mRNA exist in the bovine luteal tissue and the variant receptors are expressed at different cellular sites according to its structure. (4) Intracellular consecutive cysteine residues of LH receptor are palmitoylated and thereby inhibit internalization of the receptor. (5) As a mechanism of the bovine follicular cyst caused by stress, it is suggested that increased secretions of progesterone and cortisol from the adrenal gland exert inhibitory effects on the hypothalamus and follicle, respectively, and subsequently LH and FSH surges are blocked, then finally ovulation is suppressed and the follicle becomes cystic.

show abstract

“…In general, oocytes [2] and embryos on d1 [4] to d2 [7] after breeding are sensitive to heat stress (d0=AI). But embryos on d7 are rather resistant to heat stress [14], and therefore embryo transfer (ET) might be considered in dairy industry [1,12]. Takahashi et al reported that a higher pregnancy rate was obtained with ET after AI in repeatbreeder Holstein cattle in the northern part of Japan, Hokkaido [13].…”

mentioning

confidence: 99%

“…There are some controversial aspects of ETFAI, such as twins or embryo/fetal loss. However, if a fresh, high quality embryo could be used instead of a frozen embryo [1], ETFAI would become an innovative technology for improving reproductive performance in dairy cows during the summer period in south-western Japan.…”

mentioning

confidence: 99%

Effect of Embryo Transfer following Artificial Insemination (ETFAI) on Reproductive Performance in Dairy Cows in South-Western Japan

Tani

Hayashida²,

Tomokawa³

et al. 2010

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. In order to improve the reproductive performance during the summer period, embryo transfer (ET; Japanese black embryo) following artificial insemination (AI; Holstein semen: ETFAI) was conducted in dairy cows in south-western Japan (n=56). The conception rate was improved in cows with ETFAI compared with conventional AI, which served as the control (n=195; 30.4% vs. 13.8%, P<0.01). However, higher fetal loss was observed in ETFAI compared with the controls (38.1% vs. 7.4%, P<0.05). Four cases of twin pregnancy resulted in 2 singletons and a set of twins. There was no difference in the plasma progesterone level on d0 or d7 (d0=AI), but rather lower rectal temperature was observed on d7 or d8 (38.7C vs. 39.4C and 38.8C vs. 39.1C, P<0.05) in pregnant cows compared with those that were open. ETFAI could improve reproductive performance in dairy cows during the summer period in southwestern Japan.KEY WORDS: dairy cow, ET following AI, fetal loss, rectal temperature, summer period.

show abstract

Pregnancy rates following timed embryo transfer with fresh or vitrified in vitro produced embryos in lactating dairy cows under heat stress conditions

Cited by 72 publications

References 27 publications

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Studies on the Regulation of Expression of Luteinizing Hormone Receptor in the Ovary and the Mechanism of Follicular Cyst Formation in Ruminants

Effect of Embryo Transfer following Artificial Insemination (ETFAI) on Reproductive Performance in Dairy Cows in South-Western Japan

Contact Info

Product

Resources

About