Pregnancy Rate after Intrauterine Insemination with the Presence or Absence of Leukocytospermia in Sperms Prepared using Density Gradient Method

Abstract

Objective: To examine the association between different concentrations of leukocyte and sperm recovery rate after sperms are prepared using density gradient method and pregnancy rate after intrauterine insemination (IUI). Increased leukocytes in semen have been associated with increased reactive oxygen species (ROS) that reduces sperm quality.Methods: Semen samples that were collected from 31 male partners of couples undergoing infertility investigation were analyzed for sperm concentration, motility, and leucocytes concentration. Semen samples were then divided in two groups based on their leucocytes concentrations (category A: >0 to <1 × 10 6 /mL; category B: >1 x 10 6 /mL. Semen samples were processed using density-gradient centrifugation technique. Results:There was a significant difference in the number of sperms harvested and sperm motility after preparation. Interestingly, pregnancy rate after IUI was higher (p<0.05) in non-leukocytospermia semen (39%) when compared to leukocytospermia semen (30%). Conclusions:Seminal leukocytes (PMNL) concentration affects pregnancy rate after intrauterine insemination.

Leukospermia in Infertile Men and Successful Intrauterine Insemination at Rumah Sakit Ibu dan Anak Puri Bunda Denpasar

“…From these data it can also be said that leukospermic patients experience less success than non-leukospermic patients. 18 Infertility can be affected by a history of smoking. 19 It has been shown that smoking has a negative impact on infertility.…”

Analisis Faktor yang Mempengaruhi Keberhasilan Kehamilan pada Inseminasi Intrauterin

“…Pada wanita berusia lebih dari 40 tahun, hanya sedikit kehamilan yang tercapai setelah 3 kali inseminasi siklus. Penelitian (Widyastuti et al, 2018) menunjukkan bahwa bahkan setelah beberapa siklus inseminasi tingkat kehamilan bisa cukup baik. Studi lain, bagaimanapun, telah melaporkan penurunan yang kuat dalam angka kehamilan setelah hanya beberapa siklus.…”

The Pregnancy Rate After Intrauterine Insemination Using Semen With Different Leucocytes Concentration Prepared by Swim-Up Method

“…The optimum concentration of ROS involved in diverse physiological functions of sperm, including capacitation, acrosome reaction, and binding to the zona pellucida (Chen et al, 2013). However, it has been proven that the ROS has negatively impacted the premature acrosome reaction that correlates to the inability of rat epididymal sperm in penetrating the zona-intact of the oocyte (Widyastuti et al, 2018). the ROS level within group D may be increased, so induced the imbalance between ROS and antioxidant concentration.…”