Pregnancy outcomes using stallion epididymal sperm stored at 5 °C for 24 or 48 hours before harvest

Stawicki, R.; McDonnell, Sue M.; Giguère, S.; Turner, Regina M.

doi:10.1016/j.theriogenology.2015.10.009

Cited by 19 publications

(13 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is possible to observe a greater impact of the epididymal cooling for sperm motility rates (i.e., total, progressive, fast and medium velocities). These changes in the motility kinetics after the epididymis was stored were observed previously in stallions (Stawicki, McDonnell, Giguere, & Turner, ), dogs (Tittarelli et al., ) and cats (Ganan et al., ; Toyonaga et al., ). Motility is essential to spermatozoa as it is directly related to cell movement and consequently to the possibility of the sperm arrival in the oviduct to fertilize the oocyte (Vilela, Marquez, Graham, & Barfield, ).…”

Section: Discussionsupporting

confidence: 79%

Spermatic and oxidative profile of domestic cat (Felis catus) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)

Angrimani

Nagai

Rui

et al. 2017

Reprod Domestic Animals

View full text Add to dashboard Cite

Cooling stored epididymal samples for several days allows facilities to transport and process genetic material post-mortem. Improvements to this practice allow the preservation of sperm from domestic cats, which are the ideal study model for wild felids. However, the modifications in spermatic features and the oxidative profile are not fully understood in cats. This information is necessary for the development of biotechniques, such as new extenders for cryopreservation. Therefore, the purpose of this study was to evaluate the spermatic and oxidative profile in samples from the epididymal cauda of domestic cats cooled at 5°C for 24, 48 and 72 hr. Spermatozoa were collected from the epididymis cauda. Evaluations consisted of computer-assisted sperm analysis (CASA), plasma membrane integrity (eosin/nigrosin), acrosome integrity (fast green/rose bengal), sperm morphology, sperm DNA integrity (toluidine blue), mitochondrial activity (3'3 diaminobenzidine), activity of the antioxidant enzymes glutathione peroxidase (GPx) and superoxide dismutase (SOD), measurement of lipid peroxidation (TBARS) and protein oxidation. A decrease in sperm motility parameters was observed after 72 hr of cooling (i.e. total and progressive) with a higher percentage of minor (37.7 ± 6.3%) and total defects (53.4 ± 6.3%). Additionally, a decrease in high mitochondrial activity (Class I: 16.6 ± 2.2%) occurred after 72 hr. The decrease in motility rates after a long cooling time probably was caused by the increase in sperm abnormalities. A long cooling time causes cold shock and mitochondrial exhaustion, but there was no observed change with the oxidative stress condition. Therefore, cat epididymal sperm stored at 5°C appear to maintain a high quality for up to 48 hr of cooling time.

show abstract

Section: Discussionsupporting

confidence: 79%

Spermatic and oxidative profile of domestic cat (Felis catus) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)

Angrimani

Nagai

Rui

et al. 2017

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…Furthermore, if the retrograde flushing is performed with a freezing extender, the semen can be directly frozen and requires no further processing; however, if the retrograde flushing is performed with a cooling extender, the harvested semen needs to be centrifuged, the supernatant discarded, and the pellet resuspended in a semen freezing extender. While some authors advocate for the direct method [8], others advocate for the other approach, considering centrifugation a necessary step to activate epididymal sperm motility [18,19]. The only study comparing the two approaches failed to find any differences in post-thaw semen parameters for stallion epididymal semen processed via either approach [6].…”

Section: Introductionmentioning

confidence: 99%

Donkey Epididymal Transport for Semen Cooling and Freezing

Lago-Alvarez

Podico

Segabinazzi

et al. 2020

Animals

View full text Add to dashboard Cite

The objectives of this study were to assess the cooling and freezing of donkey epididymal semen harvested immediately after castration (Experiment 1, n = 4) or after the shipment (24 or 48 h) of epididymides attached to testicles (Experiment 2, n = 14) or dissected apart (Experiment 3, n = 36). In each experiment, semen was frozen immediately (Non-Centrif) in an egg yolk-based semen extender (EY) or after processing through cushion-centrifugation (Centrif) while extended in a skim milk-based extender (SC). In all three experiments, cooled, pre-freeze, and post-thaw epididymal semen was assessed for total motility (TM), progressive motility (PM), plasma membrane integrity (PMI), and high mitochondrial membrane potential (HMMP). Data were analyzed with R using mixed models and Tukey’s test as posthoc. Results showed that the cooling of epididymal semen up to 24 h after harvesting did not affect motility parameters or plasma membrane integrity; furthermore, in Experiment 3, the post-thaw evaluation of both Centrif and Non-Centrif achieved similar TM and PM. Collectively, the post-thaw results revealed low motility parameters across groups; while, the PMI and HMMP did not reflect this trend, and the values remained high, suggesting that there was a lack of epididymal sperm activation with either centrifugation or extenders. In summary, freshly harvested and cooled-shipped and cooled semen had satisfactory semen parameters. Future studies need to address donkey epididymal semen fertility in mares and jennies.

show abstract

“…The limitation of use of sex-sorted semen imposed reconsideration of established artificial insemination parameters such as required number of sperm per insemination (13). Preservation of epididymal sperm is one of the most important aspects in preservation of genetic material from valuable terminally ill or deceased animals (wild and domestic species) (14–18). This new approach to breeding raises fundamental physiological questions on the role of seminal plasma in fertility (19–21).…”

Section: Male Gamete Preservation and Artificial Inseminationmentioning

confidence: 99%

Grand Challenge Animal Reproduction-Theriogenology: From the Bench to Application to Animal Production and Reproductive Medicine

Tibary

2017

Front. Vet. Sci.

View full text Add to dashboard Cite

Pregnancy outcomes using stallion epididymal sperm stored at 5 °C for 24 or 48 hours before harvest

Cited by 19 publications

References 20 publications

Spermatic and oxidative profile of domestic cat (Felis catus) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)

Spermatic and oxidative profile of domestic cat (Felis catus) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)

Donkey Epididymal Transport for Semen Cooling and Freezing

Grand Challenge Animal Reproduction-Theriogenology: From the Bench to Application to Animal Production and Reproductive Medicine

Contact Info

Product

Resources

About