Pregnancy Outcomes, Immunophenotyping and Immunohistochemical Findings in a Cohort of Pregnant Patients with COVID-19—A Prospective Study

Abstract

(1) Background: SARS-CoV-2 infection during pregnancy could determine important maternal and fetal complications. We aimed to prospectively assess placental immunohistochemical changes, immunophenotyping alterations, and pregnancy outcomes in a cohort of patients with COVID-19; (2) Methods: 52 pregnant patients admitted to a tertiary maternity center between October 2020 and November 2021 were segregated into two equal groups, depending on the presence of SARS-CoV-2 infection. Blood samples, fragments of umbilical cord, amniotic membranes, and placental along with clinical data were collected. Descriptive statistics and a conditional logistic regression model were used for data analysis; (3) Results: Adverse pregnancy outcomes such as preterm labor and neonatal intensive care unit admission did not significantly differ between groups. The immunophenotyping analysis indicated that patients with moderate–severe forms of COVID-19 had a significantly reduced population of T lymphocytes, CD4+ T cells, CD8+ T cells (only numeric), CD4+/CD8+ index, B lymphocytes, and natural killer (NK) cells. Our immunohistochemistry analysis of tissue samples failed to demonstrate positivity for CD19, CD3, CD4, CD8, and CD56 markers; (4) Conclusions: Immunophenotyping analysis could be useful for risk stratification of pregnant patients, while further studies are needed to determine the extent of immunological decidual response in patients with various forms of COVID-19.

Poor Response to Gonadotropin Stimulation and Perinatal Outcomes in Fresh In Vitro Fertilization Embryo Transfer Cycles—A Retrospective Cohort Study

“…Lastly, it is worth mentioning that part of our study period (2019-2022) overlapped with the coronavirus disease 2019 (COVID-19) pandemic. Though COVID-19 infection status was not assessed in our study, previous studies have indicated no statistically significant difference between COVID-19 positive and COVID-19 negative patients regarding pregnancy outcomes (such as neonatal intensive care unit admission and stillbirth) [35,36], while others suggest an association between specific COVID-19 variants and various pregnancy complications (such as PTB) [37].…”

Predicting the Feasibility of Curative Resection in Low Rectal Cancer: Insights from a Prospective Observational Study on Preoperative Magnetic Resonance Imaging Accuracy

“…Artificial intelligence and risk stratification have been gaining more interest in the field of predictive medicine, and they have been frequently used in imaging data modeling in recent years [41][42][43][44][45][46]. Moreover, the correct reporting of positive circumferential margin on MRI has been the subject of debate.…”

Pregnancy Outcomes in Patients with Urosepsis and Uncomplicated Urinary Tract Infections—A Retrospective Study

“…Our results indicated that patients with urosepsis had an increased likelihood of premature rupture of membranes (aOR: 5.59, 95%CI: 2.02-15.40, p < 0.001) and preterm birth (aOR: 2.47, 95%CI: 1.15-5.33, p = 0.02). The role of urinary infections in the determinism of preterm birth is well established, and immunosuppressive conditions favor the development of severe forms of infection, as we determined in this study [41][42][43][44][45].…”