Pregnancy‐associated venous insufficiency course with placental and systemic oxidative stress

Ortega, Miguel Á.; Romero, Beatriz; Asúnsolo, Ángel; Martínez-Vivero, Clara; Sáinz, Felipe; Bravo, Coral; León‐Luis, Juan De; Álvarez‐Mon, Melchor; Buján, Julia; García‐Honduvilla, Natalio

doi:10.1111/jcmm.15077

Cited by 26 publications

(45 citation statements)

References 68 publications

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“…Several pathogenic mechanisms that affect the placenta in this disease have been described such as cell hypoxia, oxidative stress, extracellular matrix remodeling, and angiogenesis/lymphogenesis [12]. While CVD is a less serious condition, in prior work we also detected these changes in the placentas of women with venous insufficiency, indicating the possible impact of venous disease in these patients with CVD [9,[13][14][15]. Chronic venous insufficiency has also been associated with a series of changes in cell signaling and in the behavior of smooth muscle cells.…”

Section: Ivyspring International Publishersupporting

confidence: 52%

Lipidomic profiling of chorionic villi in the placentas of women with chronic venous disease

et al. 2020

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Background: Chronic venous disease (CVD) is a prevalent lower limb venous pathology that especially affects women, who also show an increased risk of this disease during pregnancy. Studies have shown significant structural changes in the placentas of women with CVD and several markers of tissue damage have been also described. Patients and Methods: To try to understand the different placental pathologies, research efforts have focused on examining metabolomic profiles as indicators of the repercussions of these vascular disorders. This study examines changes produced in the metabolomic profiles of chorionic villi in the placentas of women with CVD. In a study population of 12 pregnant women, 6 with and 6 without CVD, we compared through mass spectroscopy coupled to ultra-high performance liquid chromatography (UHPLC-MS), 240 metabolites in chorionic villus samples. Results: This study is the first to detect in the placental villi of pregnant women with CVD, modifications in lysophosphatidylcholines and amino acids along with diminished levels of other lipids such as triglycerides, sphingomyelins, and non-esterified omega 9 fatty acids, suggesting a role of these abnormalities in the pathogenesis of CVD. Conclusions: Our findings are a starting point for future studies designed to examine the impacts of CVD on maternal and fetal well-being.

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Section: Ivyspring International Publishersupporting

confidence: 52%

Lipidomic profiling of chorionic villi in the placentas of women with chronic venous disease

et al. 2020

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“…In prelabor rupture of fetal membranes, increased levels of these enzymes have been associated with changes in the cell cycle and in promoting oxidative stress in some placental complications ( 41 ). Recently, an association was found between CVD during pregnancy and an increase in oxidative stress markers in these placentas ( 11 ). The increased expression of these enzymes in patients with CVD suggests that lysyl oxidases could play an important role in CVD.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have observed how the placental villi of pregnant women with CVD had a significant increase in hypoxia-inducible factors, proteins related to apoptosis and oxidative stress ( 3 , 11 ). In addition, a significant increase in the number of placental villi was also observed in relation to CVD ( 3 , 11 , 19 ).…”

Section: Discussionmentioning

confidence: 99%

An increase in elastogenic components in the placental villi of women with chronic venous disease during pregnancy is associated with decreased EGFL7 expression level

et al. 2021

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Chronic venous disease (CVD) is the response to a series of hemodynamic changes in the venous system and the onset of this disease is often triggered by pregnancy. Placental tissue is particularly sensitive to the characteristic changes which occurs in venous hypertension. In this regard, changes in the extracellular matrix (ECM), that occur to adapt to this situation, are fundamental to controlling elastogenesis. Therefore, the aim of the present study was to analyze the changes that occur in the mRNA and protein expression level of proteins related to elastogenesis in the placental villi of women diagnosed with CVD, in the third trimester of pregnancy. An observational, analytical and prospective cohort study was conducted, in which the placenta from 62 women with CVD were compared with that in placenta from 52 women without a diagnosis of CVD. Gene and protein expression levels were analyzed using reverse transcription-quantitative PCR and immunohistochemistry, respectively. The results showed a significant decrease in the gene and protein expression level of EGFL7 in the placental villi of women with CVD. By contrast, significant increases in the gene and protein expression level of ECM-related proteins, such as tropoelastin, fibulin 4, fibrillin 1 and members of the lysyl oxidase family (LOX and LOXL-1) were also found in the placental villi of women with CVD. To the best of our knowledge, the results from the present study showed for the first time that CVD during pregnancy was associated with changes in the mRNA and protein expression level in essential components of the EGFL7-modulated elastogenesis process in placental villi.

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“…Antigen-antibody reactions were detected by the ABC method 45 , 46 (avidin-biotin complex) using peroxidase or alkaline phosphatase as a chromogen according to the following protocol. 1.…”

Section: Methodsmentioning

confidence: 99%

Histopathological study of JNK in venous wall of patients with chronic venous insufficiency related to osteogenesis process

Ortega¹,

Asúnsolo²,

Pekarek³

et al. 2021

Int. J. Med. Sci.

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Chronic venous insufficiency (CVI) is one of the most common vascular pathologies worldwide. One of the risk factors for the development of CVI is aging, which is why it is related to senile changes. The main trigger of the changes that occur in the venous walls in CVI is blood flow reflux, which produces increased hydrostatic pressure, leading to valve incompetence. The cellular response is one of the fundamental processes in vascular diseases, causing the activation of cell signalling pathways such as c-Jun N-terminal kinase (JNK). Metabolic changes and calcifications occur in vascular pathology as a result of pathophysiological processes. The aim of this study was to determine the expression of JNK in venous disease and its relationship with the role played by the molecules involved in the osteogenic processes in venous tissue calcification. This was a cross-sectional study that analyzed the greater saphenous vein wall in 110 patients with (R) and without venous reflux (NR), classified according to age. Histopathological techniques were used and protein expression was analysed using immunohistochemistry techniques for JNK and markers of osteogenesis (RUNX2, osteocalcin (OCN), osteopontin (OPN)). Significantly increased JNK, RUNX2, OCN, OPN and pigment epithelium-derived factor (PEDF) protein expression and the presence of osseous metaplasia and amorphous calcification were observed in younger patients (<50 years) with venous reflux. This study shows for the first time the existence of an osteogenesis process related to the expression of JNK in the venous wall.

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Pregnancy‐associated venous insufficiency course with placental and systemic oxidative stress

Cited by 26 publications

References 68 publications

Lipidomic profiling of chorionic villi in the placentas of women with chronic venous disease

Lipidomic profiling of chorionic villi in the placentas of women with chronic venous disease

An increase in elastogenic components in the placental villi of women with chronic venous disease during pregnancy is associated with decreased EGFL7 expression level

Histopathological study of JNK in venous wall of patients with chronic venous insufficiency related to osteogenesis process

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