Pregnancy after the calcium ionophore correction of pronuclei position in oocytes after intracytoplasmic sperm injection

Isachenko, Evgenia; Isachenko, Vladimir; Todorov, Plamen; Ostashko, V. F.; Kaufmann, M.; Sterzik, K.; Wiegratz, Inka

doi:10.1016/j.fertnstert.2010.04.034

Cited by 7 publications

(4 citation statements)

References 13 publications

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“…Moreover, another case study on globozoospermia associated with a lack of PLC zeta reported that artificial oocyte activation with calcium ionophores successfully overcame repeated fertilization failures [36]. There have been two other favorable oocyte activation results by calcium ionophores: patients with oligozoospermia and OAT [37,38] who had undergone repeated ICSI cycles, finally achieved fertilization and pregnancy when ICSI was coupled with calcium ionophore-mediated oocyte activation.…”

Section: Discussionmentioning

confidence: 99%

“…The fact that there were 10 cases of lost follow-up somewhat compromises the overall results; nonetheless, we believe that if the scale of this study is taken into account, the data obtained can be considered valid and valuable. Possible damage resulting from prolonged exposure of the oocytes to calcium ionophore remains a concern, particularly considering that the longest exposure time reported thus far is 20 min [38]. However, it is well known that the calcium oscillations induced by sperm entry can last from 10 to 35 min.…”

Section: Discussionmentioning

confidence: 99%

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Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

Yoon

Bae

Kim

et al. 2013

J Assist Reprod Genet

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Purpose Fertilization failures have occurred repeatedly in reproductive centers after intracytoplasmic sperm injection (ICSI) and artificial oocyte activation (AOA) has been used to prevent it. This study was performed to investigate whether spermatozoan origin influences clinical outcomes of AOAwith a calcium ionophore. Methods A total of 185 ICSI cycles with a history of no or low fertilization was included in this retrospective study. The outcomes of AOA after ICSI were compared with ejaculatednormal, ejaculated-oligo-astheno-terato or extracted-testicular spermatozoa. Results There were significant differences between the previous standard ICSI cycles and AOA cycles in the rate of fertilization and clinical outcomes among cases with different sperm origins. Thirty-eight healthy babies (20 singles and 18 twins, 29 cycles) were successfully delivered, and no congenital birth defects were observed. Conclusions Most patients with a no or low fertilization history obtained an increased fertilization rate and a positive clinical outcome with AOA regardless of the origin of spermatozoa.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

Yoon

Bae

Kim

et al. 2013

J Assist Reprod Genet

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“…Это положительно влияет на частоту оплодотворения, образования бластоцист, имплантации, наступления беременности и повышает коэффициент живорождения, причем не сказывается на частоте выкидышей, врожденных аномалий и соотношении полов у рожденных детей [13,14]. E. Isachenko et al также рассмотрели применение ионофора кальция у пациентов с нецентральным расположением пронуклеусов и отметили успешное наступление беременности при коррекции ионофором [15]. Исследователями из Турции показана эффективность применения ионофора кальция у 946 пациенток со сниженным овариальным резервом при нормальных параметрах спермы их партнеров: частота оплодотворения, имплантации, беременности и живорождения для группы с ионофором кальция и контрольной группы составили 60,7% и 55,4%, 12,8% и 10,7%, 21% и 12,8% и 10,9% и 6,1%, соответственно [16].…”

Section: б аunclassified

Наступление Беременности И Роды У Пациенток После Коррекции Расположения Пронуклеусов В Ооцитах С Использованием Ионофора Кальция

Заставский¹,

Яхьярова²,

Ким³

2023

РепроМед

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Актуальность: Непрерывное совершенствование методов вспомогательных репродуктивных технологий (ВРТ) помогает преодолеть практически все виды бесплодия. В настоящее время в репродуктивной медицине широко используется интрацитоплазматическая инъекция сперматозоидов (ИКСИ), которая позволяет получить оплодотворенные ооциты в случаях тяжелого мужского или идиопатического бесплодия. Но даже зиготы с двумя пронуклеусами не всегда могут правильно развиваться и дойти до стадии бластоцисты. Важным критерием для образования нормальной бластоцисты является центральное расположение пронуклеусов в зиготе. Поиск методов, повышающих эффективность программ ВРТ, является одним из наиболее актуальных вопросов репродуктивной медицины.Цель исследования – изучить влияние ионофора кальция на расположение пронуклеусов в зиготах, развившихся из недостаточно активированных ооцитов, и частоту наступления беременности после переноса эмбрионов на стадии бластоцисты.Методы: В статье описаны 5 клинических случаев применения ионофора кальция после неудачных программ ИКСИ. Во всех случаях отмечалось нецентральное расположение пронуклеусов в предыдущих программах. Для коррекции расположения пронуклеусов в оплодотворенных ооцитах использовался ионофор кальция после метода ИКСИ. Результаты: Наступление клинической беременности отмечалось в 80% случаев. После использования ионофора кальция 83% ооцитов были нормально оплодотворены и имели центральное расположение пронуклеусов. Также было получено 40% бластоцист хорошего и отличного качества. У 4-х пациенток наблюдалась одноплодная беременность и на данный момент все беременности окончились родами. Исход программы у 5-й пациентки остался неизвестен.Заключение: Активация ооцитов ионофором кальция после ИКСИ может быть использована для коррекции локализации пронуклеусов, что может влиять на эффективность имплантации бластоцисты и, соответственно, на благополучный исход программы ВРТ.

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“…Many studies reported that artificial oocyte activation improved fertilization and pregnancy rates in patients with histories of poor fertilization, and even no fertilization, in previous ICSI cycles ( Montag et al ., 2012 ; Yeste et al ., 2015 ). A number of research groups also reported cases of successful pregnancies in couples where ionophore was used to activate oocytes and ICSI was performed with oligoasthenoteratospermic semen ( Sugaya, 2010 ; Isachenko et al ., 2010 ), small acrosome or globozoospermic sperm ( Heindryckx et al ., 2005 ; Taylor et al ., 2010 ) and also on in vitro matured oocytes due to polycystic ovary syndrome in the women ( Kim et al ., 2015a , b ). Furthermore, this procedure was also used in cases of unexplained female infertility or diminished ovarian reserve ( Check et al ., 2010 ).…”

Section: Clinical Application For Artmentioning

confidence: 99%

Gamete activation: basic knowledge and clinical applications

Tosti

Ménézo²

2016

Hum. Reprod. Update

111

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BackgroundThe first clues to the process of gamete activation date back to nearly 60 years ago. The mutual activation of gametes is a crucial event during fertilization. In the testis and ovaries, spermatozoa and oocytes are in a state of meiotic and metabolic quiescence and require reciprocal signals in order to undergo functional changes that lead to competence for fertilization. First, the oocyte activates sperm by triggering motility, chemoattraction, binding and the acrosome reaction, culminating with the fusion of the two plasma membranes. At the end of this cascade of events, collectively known as sperm capacitation, sperm-induced oocyte activation occurs, generating electrical, morphological and metabolic modifications in the oocyte.Objective and rationaleThe aim of this review is to provide the current state of knowledge regarding the entire process of gamete activation in selected specific animal models that have contributed to our understanding of fertilization in mammals, including humans. Here we describe in detail the reciprocal induction of the two activation processes, the molecules involved and the mechanisms of cell interaction and signal transduction that ultimately result in successful embryo development and creation of a new individual.Search methodsWe carried out a literature survey with no restrictions on publication date (from the early 1950s to March 2016) using PubMed/Medline, Google Scholar and Web of Knowledge by utilizing common keywords applied in the field of fertilization and embryo development. We also screened the complete list of references published in the most recent research articles and relevant reviews published in English (both animal and human studies) on the topics investigated.OutcomesLiterature on the principal animal models demonstrates that gamete activation is a pre-requisite for successful fertilization, and is a process common to all species studied to date. We provide a detailed description of the dramatic changes in gamete morphology and behavior, the regulatory molecules triggering gamete activation and the intracellular ions and second messengers involved in active metabolic pathways in different species. Recent scientific advances suggest that artificial gamete activation may represent a novel technique to improve human IVF outcomes, but this approach requires caution.Wider implicationsAlthough controversial, manipulation of gamete activation represents a promising tool for ameliorating the fertilization rate in assisted reproductive technologies. A better knowledge of mechanisms that transform the quiescent oocyte into a pluripotent cell may also provide new insights for the clinical use of stem cells.

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Pregnancy after the calcium ionophore correction of pronuclei position in oocytes after intracytoplasmic sperm injection

Cited by 7 publications

References 13 publications

Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

Наступление Беременности И Роды У Пациенток После Коррекции Расположения Пронуклеусов В Ооцитах С Использованием Ионофора Кальция

Gamete activation: basic knowledge and clinical applications

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