Pregnancy after subzonal insemination with spermatozoa lacking outer dynein arms

Wolf, Jean-Philippe; Feneux, D.; Escalier, Denise; Rodrígues, D.; Frydman, R.; Jouannet, Pierre

doi:10.1016/0020-7292(94)90037-x

Cited by 11 publications

(13 citation statements)

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“…Even if endocrinological and functional characteristics of semen are normal, men with immotile spermatozoa are infertile. Motility may not be necessary for sperm-zona pellucida binding [14] or for penetration of the investment-free oocyte [20], but sperm motility has been considered compulsory for the penetration of cervical mucus, the movement through the female genital tract, and the entering through the oocyte investments [7].…”

mentioning

confidence: 99%

Motility Stimulant Effects of Prostasome Inclusion in Swim-Up Medium on Cryopreserved Human Spermatozoa

Carlsson

Ronquist

Stridsberg

et al. 1997

Archives of Andrology

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mentioning

confidence: 99%

Motility Stimulant Effects of Prostasome Inclusion in Swim-Up Medium on Cryopreserved Human Spermatozoa

Carlsson

Ronquist

Stridsberg

et al. 1997

Archives of Andrology

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“…Before it was possible to directly inject a single spermatozoon into the ooplasm, subzonal injection (SUZI) of numerous sperms was a successful alternative [23,24]. However, SUZI required at least minimal sperm motility in order achieve fertilization.…”

Section: Discussionmentioning

confidence: 99%

Healthy twin live-birth after ionophore treatment in a case of theophylline-resistant Kartagener syndrome

Ebner

Maurer

Oppelt

et al. 2015

J Assist Reprod Genet

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Purpose To evaluate whether it is a feasible option to target the oocyte (with Ca 2+ -ionophore) in case that sperm motility cannot be restored in Kartagener syndrome. Methods A case of a male Kartagener syndrome with exclusively immotile spermatozoa that did not react to the dimethylxanthine theophylline. Thus, half of the associated oocytes were treated for 15 min with the ready-to-use-ionophore CultActive immediately after ICSI whereas the other 50 % were injected with routine ICSI without artificial oocyte activation. Rates of fertilization, blastulation, pregnancy and live birth were evaluated. Results Fertilization check revealed that none of the conventionally injected but 4/6 (66.7 %) of the artificially activated oocytes showed two pronuclei. Three embryos were of good and one of fair quality. Corresponding blastocyst formation rate was 3 out of 4 (75 %). A double embryo transfer led to a healthy twin birth in the 34th week of gestation (two boys with a birth weight of 1724 g and 2199 g). Conclusions This case indicates that Ca 2+-ionophore treatment in cycles from theophylline-resistant Kartagener syndrome patients is a feasible option. The future will show if routine application of A23187 in Kartagener or primary cilia dyskinesis patients will be of benefit.

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“…Conventional IVF may fail in certain couples with impaired sperm motility and when the number of motile sperm present in the ejaculate is too low. Since the immotile sperm have a normal ability to acrosome react in vitro, immotile sperm pregnancies and live births have been reported following subzonal insemination (SUZI) or partial zona dissection (PZD) [9,15,17,24,25].…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural Analysis of Tese and Semen Sperm Tails From Patients Exhibiting Absolute Immotility on the Day of Icsi

Evirgen

Özgüner

Demirel

et al. 2003

Archives of Andrology

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Pregnancy after subzonal insemination with spermatozoa lacking outer dynein arms

Cited by 11 publications

References 0 publications

Motility Stimulant Effects of Prostasome Inclusion in Swim-Up Medium on Cryopreserved Human Spermatozoa

Motility Stimulant Effects of Prostasome Inclusion in Swim-Up Medium on Cryopreserved Human Spermatozoa

Healthy twin live-birth after ionophore treatment in a case of theophylline-resistant Kartagener syndrome

Ultrastructural Analysis of Tese and Semen Sperm Tails From Patients Exhibiting Absolute Immotility on the Day of Icsi

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