Pregnancies established from water buffalo () blastocysts derived from in vitro matured, in vitro fertilized oocytes and co-cultured with cumulus and oviductal cells

Madan, M. L.; Chauhan, M. S.; Singla, S. K.; Manik, R. S.

doi:10.1016/0093-691x(94)90376-t

Cited by 51 publications

(41 citation statements)

References 17 publications

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“…In earlier studies involving bubaline embryos, development to the blastocyst stage in vitro was demonstrated when cultured in ligated rabbit (Chantaraprateep et a., 1989), sheep oviduct (Galli et al, 1998), co-cultured with oviduct epithelial cells and/or cumulus cells (Totey et al, 1992;Madan et al, 1994;Ocampo et al, 2001c), Buffalo Rat Liver cells or Vero cells (Boni et al, 1994(Boni et al, , 1999Gasparrini, 2002). Although the embryotrophic properties of these cells have been demonstrated, the exact role on the acquisition of embryos developmental potential remained unclear.…”

Section: Resultsmentioning

confidence: 99%

Developmental competence of water buffalo oocytes in a synthetic oviductal fluid base medium

2016

Res. Opin. Anim. Vet. Sci.

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In this study, in vitro derived (oocytes from ovaries of slaughtered buffalo cows) and in vivo derived (oocytes collected through TUFA from living buffalo cows) oocytes were assessed on their ability to acquire the necessary developmental competence in the production of blastocyst stage embryos when cultured in culture systems using a complex/serial culture media or a single based medium from maturation to culture. In vitro derived oocytes ability to complete the 1 st meiotic division (nuclear maturation) showed no difference when cultured in either culture systems (82.6-83.1%) but was higher compared to in vivo derived oocytes (62.3-74.4%). The cleavage rate of in vivo derived oocytes (78.7-79.2%) was significantly higher than in vitro derived oocytes (59.4-62.9%). Significantly higher blastocyst formation rate was observed from in vivo derived oocytes cultured in a single based culture system (31.1%). Overall, the results showed the usefulness and practicality of using a single synthetic oviductal fluid based medium for supporting buffalo oocytes acquisition of developmental competence in an in vitro culture system.

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Section: Resultsmentioning

confidence: 99%

Developmental competence of water buffalo oocytes in a synthetic oviductal fluid base medium

2016

Res. Opin. Anim. Vet. Sci.

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“…Earlier studies have used buffalo oviductal epithelial cell co-culture system to support embryonic development up to the blastocyst stage (Chuangsoongneon and Kamonpatana, 1991;Jainudeen et al, 1993;Totey et al, 1992), but with poor efficiency (8.2%). The inclusion of cumulus cell monolayer with oviductal epithelial cells improved the blastocyst formation rate of cleaved embryos (Madan et al, 1994a). Others have successfully used buffalo rat liver cell monolayer (Boni, 1995;, Vero cells (Gasparrini, 2002) or chick embryo amniotic fluid (Ocampo et al, 1994), which were attributed to the secretion of embryotrophic factors like growth factors-I (e.g., IGF-I) (Narula et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…Despite this progress, however, the proportion of buffalo eggs being fertilized after IVM remained low compared to cattle (Madan et al, 1994a;Ocampo et al, 1996a;Gasparrini, 2002). A major limiting factor is the source and quality of frozen semen, thus requires testing of several bulls to accurately identify the few suitable for IVF (Aquino et al, 2015;Ocampo and Ocampo, 2015).…”

Section: Introductionmentioning

confidence: 99%

Factors influencing the maturation, fertilization and development of swamp buffalo oocyte in vitro

Ocampo¹,

Rigos²,

Ocampo³

2016

Res. Opin. Anim. Vet. Sci.

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Buffalo ovaries (n=610) were collected from the abattoir and the oocytes recovered were matured, fertilized and the resulting zygotes were cultured in vitro. In Experiment 1, the maturation and fertilization rates of O 1 -O 2 oocytes were higher than O 3 -O 5 oocytes. In Experiment 2, the two batches of TCM-199 medium used with different hormonal supplementation did not influence the maturation and fertilization of the oocytes at 70.7-86.7% and 59.4-66.0% respectively. In Experiment 3, bull variability was observed in terms of their capacity for fertilization. In Experiment 4, higher fertilization rate was observed in response to caffeine (3-10 mg/ml) compared to control. In Experiment 5, 1 × 10 6 sperm/ml concentration during fertilization had a lower penetration rate (43.0%) than 5, 10 × 10 6 sperm/ml concentration (68.4-71.0%). In Experiment 6, development to the blastocyst stage in TCM-199 or mSOF medium with or without cumulus cell monolayer had no significant difference (11.5-22.2%) with total cell count ranging from 84.6±11.2 to 91.4±12.2. Overall, the results showed the importance of cumulus cells in the acquisition of developmental competence of oocytes maturing in vitro and its subsequent fertilization. That, selection of appropriate bull, its optimum sperm concentration, the choice of capacitating agent and its concentration, the type of medium for culture of resulting zygotes is critical for successful application of in vitro maturation, fertilization and culturing for mass production of buffalo embryos. Keywords: Embryos; fertilization; maturation; oocytes To cite this article: Ocampo LC, LM Rigos and MB Ocampo, 2015. Factors influencing the maturation, fertilization and development of swamp buffalo oocyte in vitro. Res. Opin. Anim. Vet. Sci., 6(1): 24-31.

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“…However, they have low fertility rate which may be attributed to low follicular number in the ovary, low response to superovulation and high number of atretic follicles. Recently, efforts are established to enhance the reproductive efficiency of these animals using reproductive biotechnology such as in vitro fertilization (IVF) technology and embryo transfer (ET) [1].…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Culture Conditions on In vitro Development and Quality of Buffalo Embryos

Ibrahim

Mansour

Labib

et al. 2018

Zagazig Veterinary Journal

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This work was carried out to evaluate the quality and the developmental competence of the in vitro buffalo embryos produced under different culture conditions. Oocytes were matured in TCM 199 for 24 h at 38.5°C and 5% CO 2 . After maturation, oocytes were in vitro fertilized by frozen thawed sperms capacitated in vitro by caffeine at final concentration 2 million sperm /ml. The sperm-oocytes were incubated in TALP medium for 24 hrs. Fertilized oocytes were cultured in Cell free media (CR1aa) or with cumulus or oviductal cell monolayers (Experiment 1), CR1aa with addition of (10, 20, 50 and 100 µg/mL) lactoferrin (Experiment 2) inside CO 2 incubator with 5% CO 2 at 38°C. The cleavage, morula, blastocyst rates were recorded and embryo cell number and mitochondrial function were evaluated. Addition of bovine oviductal cell monolayer (BOCM) to culture media significantly increased the morula and the blastocyst compared with cell free media and media with cumulus cell monolayer and also significantly increased the cell number and the mitochondrial function of in vitro produced buffalo embryos. Addition of lactoferrin by concentration 50µg/ml to culture media resulted in a significant increase in rates of cleavage; morula and blastocyst compared with control or other concentrations and also resulted in an increase in the cell number and the mitochondrial function of in vitro produced buffalo embryos. It could be concluded that, addition of oviductal cells (monolayer) or lactoferrin to culture medium significantly improved the quality of in vitro produced buffalo embryos.

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Pregnancies established from water buffalo () blastocysts derived from in vitro matured, in vitro fertilized oocytes and co-cultured with cumulus and oviductal cells

Cited by 51 publications

References 17 publications

Developmental competence of water buffalo oocytes in a synthetic oviductal fluid base medium

Developmental competence of water buffalo oocytes in a synthetic oviductal fluid base medium

Factors influencing the maturation, fertilization and development of swamp buffalo oocyte in vitro

Effect of Different Culture Conditions on In vitro Development and Quality of Buffalo Embryos

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