“…Studies have shown that valproic acid can induce tissue pathological damage associated with autophagy by modulating the AMPK/mTOR signaling pathway ( Alsemeh et al, 2022 ). And another type of AEDs Pregabalin can reduce the number of germ cells while increasing the number of interstitial cells, and cause a decrease in the number and diameter of convoluted tubules ( Salem et al, 2020 ). It also induces cell apoptosis, increases caspase-3 expression in the testis ( Shokry et al, 2020 ), upregulates pro-apoptotic genes BAX and p38 MAPK, and downregulates the anti-apoptotic gene BCL2 ( Taha et al, 2020 ).…”