Prefrontal Catecholaminergic Turnover and Antioxidant Defense System of Chronically Stressed Rats

Popović, Nataša; Pajović, SnežanaB.; Stojiljković, Vesna; Pejić, Snežana; Todorović, Ana; Pavlović, Ivan; Gavrilović, Ljubica

doi:10.3409/fb65_1.43

Cited by 9 publications

(16 citation statements)

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“…Eleven-week-old Wistar male rats (300-340 g) were maintained under standard laboratory conditions with water and food ad libitum and kept three to four per cage [20]. The care was taken to minimize the pain and discomfort of the animals according to the recommendations of the Ethical Committee of the Vinča Institute of Nuclear Sciences, Belgrade, Serbia, which follows the guidelines of the registered “Serbian Society for the Use of Animals in Research and Education.” In accordance with our previous protocol [21], animals were divided into three groups: CRS group ( n = 20) consisted of animals exposed to chronic restraint stress treatment and CRS+Li group ( n = 20) consisted of animals exposed to chronic restraint stress treatment with Li given each day immediately prior to daily restraint. Restraint stress was performed by placing each animal in a 25 × 7 cm plastic bottle as described previously [22].…”

Section: Methodsmentioning

confidence: 99%

“…The reaction was performed in an incubation mixture containing 1 M Tris-HCl, 5 mM EDTA, pH 8.0 and monitored spectrophotometrically at 230 nm. One unit of CAT activity was defined as 1 μ mol of H 2 O 2 decomposed per minute under the assay conditions [21].…”

Section: Methodsmentioning

confidence: 99%

“…The oxidation of NADPH was monitored spectrophotometrically as a decrease in absorbance at 340 nm. One GPx-340 unit was defined as 1 μ mol of NADH oxidized per minute under the assay conditions [21].…”

Section: Methodsmentioning

confidence: 99%

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Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium

Popović

Stojiljković

Pejić

et al. 2019

Oxidative Medicine and Cellular Longevity

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This study examined the effects of lithium on gene expression and activity of the antioxidant enzymes copper zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the hippocampus of chronically stressed rats. In addition, we examined the effects of lithium on anxiety behaviors, hippocampal concentrations of dopamine (DA) and malondialdehyde (MDA), protein levels of brain-derived neurotrophic factor (BDNF), tyrosine hydroxylase (TH), dopamine transporter (DAT), and catechol-O-methyltransferase (COMT), as well as activity of monoamine oxidase (MAO) in chronically stressed rats. The investigated parameters were quantified by real-time RT-PCR, Western blot analyses, and assays of enzyme activities. We found that lithium did not change gene expression of SOD1, CAT, GPx, and GR but decreased gene expression of SOD2 in chronically stressed rats. A very important result in this study was that lithium treatment decreased the enzyme activities of SOD1 and SOD2 but increased the enzyme activities of GPx and GR in stress condition, which indicates the control of redox balance. The reduced concentration of MDA confirms this. In addition, we found that lithium treatment decreased high protein levels of BDNF and DAT in chronically stressed rats to the level found in unstressed animals. Also, lithium treatment increased the expression of TH but decreased the enzyme activity of MAO B, which contributed to the increase of hippocampal concentration of DA in chronically stressed rats to the level of unstressed animals. Finally, lithium treatment in animals exposed to chronic stress increased the time spent in open arms. Lithium-induced modulation of hippocampal antioxidant status and attenuation of oxidative stress stabilized behavior in animals with high anxiety index. In addition, reduced oxidative stress was followed by the changes of both turnover of DA and levels of BDNF protein in chronically stressed rats treated with lithium. These findings may be important in preclinical research of the effects of lithium on oxidative stress level in pathological conditions.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium

Popović

Stojiljković

Pejić

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Moreover, CRS affects the molecular mechanisms of catecholaminergic turnover in the brain ( Popović et al, 2017 ). It is important to examine the expression of key enzymes involved in catecholamine biosynthesis, reuptake, and degradation ( Kvetnansky et al, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary-Adrenal Axis, Serotonin Turnover Systems, and ERK/Akt Phosphorylation

Yoo

Kim

et al. 2018

Front. Pharmacol.

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The leaves of Vaccinium bracteatum Thunb. are a source of traditional herbal medicines found in East Asia. The present study aimed to evaluate the mechanisms underlying the antidepressant-like effects of water extract of V. bracteatum Thunb. leaves (VBLW) in a mouse model of chronic restraint stress (CRS) and to identify the possible molecular in vitro mechanisms of the neuroprotective effects. The CRS-exposed mice were orally administered VBLW (100 and 200 mg/kg) daily for 21 days consecutively. The behavioral effects of VBLW were assessed through the forced swim test (FST) and the open field test (OFT). The levels of serum corticosterone (CORT), corticotropin releasing hormone (CRH), and adrenocorticotropin hormone (ACTH), brain monoamines, such as serotonin, dopamine, and norepinephrine, and serotonin turnover by tryptophan hydroxylase 2 (TPH2), serotonin reuptake (SERT), and monoamine oxidase A (MAO-A) were evaluated, in addition to the extracellular signal-regulated kinases (ERKs)/protein kinase B (Akt) signaling pathway. CRS-exposed mice treated with VBLW (100 and 200 mg/kg) showed significantly reduced immobility time and increased swimming and climbing times in the FST, and increased locomotor activity in the OFT. Moreover, CRS mice treated with VBLW exhibited significantly decreased CORT and ACTH, but enhanced brain monoamine neurotransmitters. In addition, CRS mice treated with VBLW had dramatically decreased protein levels of MAO-A and SERT, but increased TPH2 protein levels in the hippocampus and the PFC. Similarly, VBLW significantly upregulated the ERKs/Akt signaling pathway in the hippocampus and the PFC. Furthermore, VBLW showed neuroprotective effects via increased CREB phosphorylation in CORT-induced cell injury that were mediated through the ERK/Akt/mTOR signaling pathways. These results suggested that the antidepressant-like effects of VBLW might be mediated by the regulation of the HPA axis, glucocorticoids, and serotonin turnover, such as TPH2, SERT, and MAO-A, as well as the concentration of monoamine neurotransmitters, and the activities of ERK and Akt phosphorylation, which were possibly associated with neuroprotective effects.

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“…Wistar male rats (11-week-old) were kept under standard laboratory conditions with water and food ad libitum, three to four per cage [13]. In accordance with our previous protocol [14] animals were divided into two groups: the CONTROL group (n=10) was not exposed to any treatment and the CRS group (n=10) consisted of animals exposed to the treatment of chronic restraint stress. Animals in CRS groups were exposed to 2h of restraint stress every day, for a period of 14 days, which is in accordance with the protocol of Popović et al [14].…”

Section: Animals and Stress Modelsmentioning

confidence: 99%

Interrelationship of Prefrontal Brain-Derived Neurotrophic Factor and Neuroendocrine System During Chronic Restraint Stress

Popović¹,

Stojiljković²,

Pejić³

et al. 2020

RAP 2019 Conference Proceedings

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The hypothalamic-pituitary-adrenal (HPA) axis plays an important role in the adaptation of the organism to stress. Because of a key role of neuroendocrine system in response to a stressful situation, as well as a significant impact of stress on neuronal plasticity, in this work we investigated how chronic restraint stress (CRS: 2 hours × 14 days) affected the protein levels of BDNF in the prefrontal cortex (PFC), as well as the concentration of adrenocorticotropic hormone (ACTH) and corticosterone (CORT) in the plasma. In addition, the aim of this study was to determine a possible correlation between levels of BDNF in the PFC and plasma CORT levels of animals exposed to CRS. We found that CRS increases levels of prefrontal BDNF protein by 25% and levels of CORT by 280%, but decreases levels of ACTH by 18%. Also, we recorded a low, but significant positive correlation between prefrontal BDNF levels and concentrations of CORT in the plasma of chronically stressed rats. Our data confirm that prefrontal BDNF might be an important regulator involved in the adaptive strategy of the HPA axis to maintain adequate reactivity in stress conditions provoked by CRS.

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Prefrontal Catecholaminergic Turnover and Antioxidant Defense System of Chronically Stressed Rats

Cited by 9 publications

References 49 publications

Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium

Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium

Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary-Adrenal Axis, Serotonin Turnover Systems, and ERK/Akt Phosphorylation

Interrelationship of Prefrontal Brain-Derived Neurotrophic Factor and Neuroendocrine System During Chronic Restraint Stress

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