Preformed confluent endothelial cell monolayers prevent early platelet deposition on vascular prostheses in baboons

Schneider, Peter A.; Hanson, Stephen R.; Price, Todd M.; Harker, Laurence A.

doi:10.1016/0741-5214(88)90272-8

Cited by 60 publications

(18 citation statements)

References 18 publications

(2 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is in agreement with data reported previously. 5,8,[22][23][24][25][26] On seeded substrates, however, the number of platelets deposited on the substrates under stationary conditions slightly increased with endothelial cell density. Although similar numbers of ECs adhered to both uncoated and coated PS-CO 2 (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Coating of surfaces with von Willebrand factor, fibrinogen, or fibronectin increases adhesion of platelets, whereas coating with albumin or plasma decreases adhesion of platelets. [18][19][20][21][22] Seeding of ECs on artificial surfaces may result in decreased platelet deposition in dogs, 23 baboons, 24 and humans, 5,8,25 although at least one study failed to show this effect. 26 Decreased numbers of platelets adhering to endothelialized surfaces may be explained by the production of PGI 2 and nitric oxide by ECs.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Blood compatibility of surfaces with immobilized albumin-heparin conjugate and effect of endothelial cell seeding on platelet adhesion

Bos

Scharenborg

Poot

et al. 1999

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Endothelial cell (EC) seeding significantly improves the blood compatibility of artificial surfaces. Although a coating consisting of albumin and heparin (alb-hep) is a suitable substrate for seeded ECs, binding of ECs to the substrate further improves when small amounts of fibronectin are present in the alb-hep coating. Alb-hep conjugate was immobilized on carbon dioxide gas plasma-treated polystyrene (PS-CO(2)), thereby significantly increasing the recalcification time of blood plasma exposed to this surface. Furthermore, surface-immobilized alb-hep conjugate inhibited exogenous thrombin. Heparin activity was reduced by adding fibronectin on top of a monolayer of alb-hep conjugate, but not by simultaneous coating of fibronectin and alb-hep conjugate. Coating of PS-CO(2) with alb-hep conjugate significantly decreased contact activation (FXII activation). The number of platelets deposited from blood plasma on PS-CO(2) coated with alb-hep conjugate was twice as high as on PS-CO(2) coated with albumin. Addition of fibronectin to alb-hep conjugate-coated PS-CO(2) had no significant effect on the number of adhered platelets. Seeding of the substrates with ECs significantly reduced the number of adhered platelets under stationary conditions. Platelets deposited onto endothelialized surfaces were primarily found on endothelial cell edges, and sparingly on areas between ECs. In conclusion, alb-hep conjugate-coated surfaces display anticoagulant activity. ECs adhering to and proliferating on this coating significantly decrease the number of platelets which adhere to the surface. Therefore, alb-hep conjugate-coated surfaces form a suitable substrate for seeding of ECs in low density. Although application of fibronectin on top of the coating decreases the anticoagulant activity to some extent, it might be useful in view of the improved adherence of ECs to the coating.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Blood compatibility of surfaces with immobilized albumin-heparin conjugate and effect of endothelial cell seeding on platelet adhesion

Bos

Scharenborg

Poot

et al. 1999

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

show abstract

“…Significantly increased patency rates [22][23][24][25][26] and a strong reduction in platelet adhesion and thrombus formation [27][28][29] were observed after endothelial cell seeding of vascular grafts.…”

Section: Endothelial Ceu Seeding Of Synthetic Vascular Graftsmentioning

confidence: 99%

“…Modification of existing graft materials by chemical surface treatment [33], glow discharge modification [30,[34][35][36] or application of a suitable protein coating may improve the potential of currently available graft materials for endothelial cell adhesion and proliferation. Regarding protein coating, substances like fibrin glue [37,38], non-crosslinked collagen [28,39] and fibronectin [40,41] have been shown to result in improved in vitro endothelialization of vascular graft materials. Furthermore, immobilization of RGD-peptides [42] or antibodies against endothelial cell membrane antigens [43] may improve adherence and growth of seeded endothelial cells.…”

Section: Substrate Compatffiilitymentioning

confidence: 99%

Tissue-Engineered Vascular Grafts for Small-Diameter Arterial Replacement

Wissink

Feijén

2002

Polymer Based Systems on Tissue Engineering, Replacement and Regeneration

View full text Add to dashboard Cite

“…Restoration of a functional pulmonary vasculature will require reestablishing intact, i.e., quiescent and nonthrombogenic, endothelial cell monolayers lining the entire vascular tree throughout the complex decellularized scaffold (17,95). We posit that successful bioengineering of functional lungs and their clinical usefulness will depend on innovative approaches to restoring a functional pulmonary vasculature.…”

mentioning

confidence: 99%