Preferred Growth Orientation of Apatite Crystals on Biological Hydroxyapatite Enriched with Bioactive Glass: A Biomimetic Behavior

Rincón-López, July Andrea; Hermann-Muñoz, Jennifer Andrea; Cinca-Luis, Núria; Garrido, Beatriz; Cano, I.G.; Guilemany, J.M.; Alvarado-Orozco, J.M.; Muñoz-Saldaña, J.

doi:10.1021/acs.cgd.9b00268

Cited by 17 publications

(18 citation statements)

References 65 publications

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“…The mechanism of growth of apatite structures on compact discs can be understood by the chemistry and dynamics of the surface charges that build upon a surface when immersed in a fluid 26 , 27 . In most of the cases, it has been seen that the apatite nucleation starts with ions being nucleated on the surface resulting in the formation of Ca rich surface, which is positively charged 28 . This positively charged surface forms the base layer for drawing the negatively charged ions by electrostatic interactions.…”

Section: Resultsmentioning

confidence: 99%

Microstructural, electrical and biological activity in $$\mathrm{Ca}_{10}(\mathrm{PO}_4)_6(\mathrm{OH})_2-\mathrm{Ba}_{0.5}\mathrm{Sr}_{0.5}\mathrm{TiO}_3$$ ceramic composites designed for tissue engineering applications

Das

Pamu

Bhardwaj

et al. 2021

Sci Rep

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The article investigates electrically active ceramic composite of $$\mathrm{Ca}_{10}(\mathrm{PO}_4)_6(\mathrm{OH})_2$$ Ca 10 ( PO 4 ) 6 ( OH ) 2 (HAP) and $$\mathrm{Ba}_{0.5}\mathrm{Sr}_{0.5}\mathrm{TiO}_{3}$$ Ba 0.5 Sr 0.5 TiO 3 (BST) for biomedical applications. The study is a systematic blend of the materials science aspect of composites with a special focus on the dielectric and biological properties and their relationships. The article emphasized primarily extracting the dielectric constant ($$\epsilon _r)$$ ϵ r ) of the specimens (that lay in the range of 3–65) and related them to microstructural properties like the grain size and at.% of BST. A broad outlook on the importance of $$\epsilon _r$$ ϵ r in determining the suitability of bioceramics for clinical applications is presented. Bioactivity analysis of the specimens led to probing the surface charges (that were negative), and it was found crucial to the growth of dense apatite layers. Furthermore, the cytocompatibility of the specimens displayed cell viability above 100% for Day 1, which increased substantially for Day 3. To reveal other biological properties of the composites, protein adsorption studies using bovine serum albumin (BSA) and fetal bovine serum (FBS) was carried out. Electrostatic interactions govern the adsorption, and the mathematical dependence on surface charges is linear. The protein adsorption is also linearly correlated with the $$\epsilon _r$$ ϵ r , intrinsic to the biomaterials. We delve deeper into protein–biomaterials interactions by considering the evolution of the secondary structure of BSA adsorbed into the specimens. Based on the investigations, 20 at.% HAP–80 at.% BST (20H–80B) was established as a suitable composite comprising the desired features of HAP and BST. Such explorations of electrical and biological properties are interesting for modulating the behavior of bioceramic composites. The results project the suitability of 20H–80B for designing electrically active smart scaffolds for the proposed biomedical applications and are expected to incite further clinical trials.

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Section: Resultsmentioning

confidence: 99%

Microstructural, electrical and biological activity in $$\mathrm{Ca}_{10}(\mathrm{PO}_4)_6(\mathrm{OH})_2-\mathrm{Ba}_{0.5}\mathrm{Sr}_{0.5}\mathrm{TiO}_3$$ ceramic composites designed for tissue engineering applications

Das

Pamu

Bhardwaj

et al. 2021

Sci Rep

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“…The closeness and similarity between both structures promote the growth of a bone-like apatite structure aligned to the main crystallographic planes of SC minimizing the nucleation barrier and accelerating its crystallization process as observed after 6 days of immersion (Fig 5) in comparison with the crystallization process of other calcium phosphates in HAp 36 . Both phases exhibit very close main diffraction peaks, as can be observed in Fig.…”

mentioning

confidence: 87%

“…Bone-like apatite formation was assessed following the protocols and characterization techniques established in a previous study 36 . Summarizing, SC ceramics were immersed in 25 mL of a modified Hank's solution (H8264, Sigma-Aldrich, Germany) for periods of 0, 6, 14, and 28 days at 37 ºC in static conditions, replacing the solution every 3 rd day.…”

Section: Bioactivity and Degradation Assessmentmentioning

confidence: 99%

“…For the HRTEM image simulation, atomic models were created using RHODIUS 38 , and images were simulated using the TEM-SIM software 39 . The procedure reported in 36 was employed for TEM samples preparation and measurement. In a brief description, SC ceramics were ground in an agate mortar, and the resulting powder was exposed to Hank´s solution for 0, 6, and 28 days.…”

Section: Structurementioning

confidence: 99%

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Apatite Mineralization Process from Silicocarnotite Bioceramics: Mechanism of Crystal Growth and Maturation

Rincón-López

Hermann-Muñoz

Cinca-Luis

et al. 2020

Crystal Growth & Design

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A mechanism for the formation and crystallization processes of bone-like apatite grown on non-stoichiometric silicocarnotite (SC) is here proposed. Single-phase SC powders and ceramics were obtained from fixed mixtures of hydroxyapatite and bioactive glass 45S5. The bioactive behavior of SC was assessed by immersion in Hank´s solution at different times.Afterward, a systematic theoretical-experimental study of the structural properties at the micro and nanoscale using TEM was performed and correlated with SEM, EDX, XRD, and Raman techniques to determine the apatite mineralization process from the SC phase. The initial stage of apatite formation from SC was identified as the hydration and further polymerization of silanol groups, resulting in a silica-based hydrogel, which plays a critical role in the ionic exchange. As a result of the adsorption of ionic species from the medium into the silica-based hydrogel, the precipitation of crystalline apatitic structures starts through the emergence of newly formed SC nanocrystals, which act as a template for the crystallization process of a substituted apatite with SC-like structure. Then, due to the polymorphism between SC and HAp structures, the apatite layer retains the SC periodic arrangement following an epitaxial-like growth mechanism. Identification of the apatite layer formation mechanism is critical to understand its physical and chemical properties, which controls the long-term dissolution/precipitation rate of bioactive materials and their performance in the biological environment.

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“…The charge distributions and surface topographical structures of the adsorbate and the adsorbent have to match for enhanced adsorption. A particularly oriented crystalline hydroxyapatite surface was also reported to assist certain biological functions [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Enhanced model protein adsorption of nanoparticulate hydroxyapatite thin films on silk sericin and fibroin surfaces

Özcan

Çiftçioğlu

2021

J Mater Sci: Mater Med

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Hydroxyapatite coated metallic implants favorably combine the required biocompatibility with the mechanical properties. As an alternative to the industrial coating method of plasma spraying with inherently potential deleterious effects, sol-gel methods have attracted much attention. In this study, the effects of intermediate silk fibroin and silk sericin layers on the protein adsorption capacity of hydroxyapatite films formed by a particulate sol-gel method were determined experimentally. The preparation of the layered silk protein/hydroxyapatite structures on glass substrates, and the effects of the underlying silk proteins on the topography of the hydroxyapatite coatings were described. The topography of the hydroxyapatite layer fabricated on the silk sericin was such that the hydroxyapatite particles were oriented forming an oriented crystalline surface. The model protein (bovine serum albumin) adsorption increased to 2.62 µg/cm2 on the latter surface as compared to 1.37 µg/cm2 of hydroxyapatite on glass without an intermediate silk sericin layer.

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Preferred Growth Orientation of Apatite Crystals on Biological Hydroxyapatite Enriched with Bioactive Glass: A Biomimetic Behavior

Cited by 17 publications

References 65 publications

Microstructural, electrical and biological activity in $$\mathrm{Ca}_{10}(\mathrm{PO}_4)_6(\mathrm{OH})_2-\mathrm{Ba}_{0.5}\mathrm{Sr}_{0.5}\mathrm{TiO}_3$$ ceramic composites designed for tissue engineering applications

Microstructural, electrical and biological activity in $$\mathrm{Ca}_{10}(\mathrm{PO}_4)_6(\mathrm{OH})_2-\mathrm{Ba}_{0.5}\mathrm{Sr}_{0.5}\mathrm{TiO}_3$$ ceramic composites designed for tissue engineering applications

Apatite Mineralization Process from Silicocarnotite Bioceramics: Mechanism of Crystal Growth and Maturation

Enhanced model protein adsorption of nanoparticulate hydroxyapatite thin films on silk sericin and fibroin surfaces

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