Preferential Usage of TCR-Vβ17 by Peripheral and Cutaneous T Cells in Nickel-Induced Contact Dermatitis

Büdinger, Lioba; Neuser, Nicole; Totzke, Uwe; Merk, Hans F.; Hertl, Michael

doi:10.4049/jimmunol.167.10.6038

Cited by 57 publications

(45 citation statements)

References 31 publications

(24 reference statements)

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“…In a more recent study, NiSO 4 caused a positive LTT in samples from most adults, regardless of the presence or absence of a positive patch test or clinical Ni allergy [40], casting further doubt on the usefulness of LTT for Ni. There is, however, evidence that identification of certain T-cell receptors for Ni may improve the correlation between in vitro analysis and clinical manifestations [41][42][43].…”

Section: Ltt For the Diagnosis Of Metal-induced Allergic Disordersmentioning

confidence: 99%

Diagnostic relevance of the lymphocyte transformation test for sensitization to beryllium and other metals (IUPAC Technical Report)

Klein¹,

Schwenk²,

Heinrich‐Ramm³

et al. 2004

Pure and Applied Chemistry

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The lymphocyte transformation test (LTT) has been proven useful especially in the diagnosis of drug-induced allergic disorders. It is an in vitro test which is based on the fact that lymphocytes, which have been sensitized by a certain antigen, transform into blasts and proliferate when they are again exposed to this antigen. This proliferation is determined by measurement of the incorporation of [3H ]-thymidine or bromodeoxyuridine into replicating DNA. The test has the advantage over skin tests of avoiding re-exposure of individuals, and it was, therefore, hoped that it may also help to diagnose metal allergies and especially sensitization toward beryllium. However, the LTT measures only the sensitization of lymphocytes, but not the effector reaction, i.e., there may be positive results in exposed individuals even in the absence of clinical symptoms. There are several reports evaluating the LTT toward gold salts (Au), amalgam (Hg), nickel (Ni), beryllium (Be), and several other metals. With metals other than Be, the LTT appears to be of little use. In contrast, the LTT with Be may, indeed, define patients at risk of developing chronic beryllium disease (CBD), which affects mainly the respiratory tract and may even cause death. Beryllium sensitization progresses to CBD at a rate of 7-11 % per year. Since the Be-LTT can detect sensitization in workers who have not yet developed a disease it is an important diagnostic tool to detect individuals at risk. In conclusion, the LTT can detect a cell-mediated immunological response of an individual to metals. However, for most metals its usefulness is questionable, with the exception of Be; a positive Be-LTT can identify not only patients with CBD, but also persons at risk of developing CBD in later years.

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Section: Ltt For the Diagnosis Of Metal-induced Allergic Disordersmentioning

confidence: 99%

Diagnostic relevance of the lymphocyte transformation test for sensitization to beryllium and other metals (IUPAC Technical Report)

Klein¹,

Schwenk²,

Heinrich‐Ramm³

et al. 2004

Pure and Applied Chemistry

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“…Büdinger et al (7) showed preferential usage of TCR-V␤ 17 by peripheral and cutaneous T cells in patients with nickel-induced contact dermatitis. Kuwana et al (8) showed highly restricted TCR-V␤ usage by autoreactive human T cell clones specific for DNA topoisomerase I in patients with systemic sclerosis.…”

Section: Tcr Rearrangement In Lymphocytes Infiltrating Melanoma Metasmentioning

confidence: 99%

TCR Rearrangement in Lymphocytes Infiltrating Melanoma Metastases After Administration of Autologous Dinitrophenyl-Modified Vaccine

Manne

Mastrangelo

Sato

et al. 2002

The Journal of Immunology

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Administration of a vaccine consisting of autologous melanoma cells modified with a hapten, dinitrophenyl (DNP), induces T cell infiltration of metastatic sites. We have reported an analysis of these infiltrating T cells, indicating that certain TCR-Vβ gene segments are greatly overexpressed. In this study, we investigate the rearrangement of the TCR-Vβ as well as the junctional diversity in T cells infiltrating melanoma metastases following treatment with DNP vaccine. In 19 of 26 control specimens, V-D-J length analysis showed the expected polyclonal patterns. In contrast, postvaccine tumors from 9 of 10 patients showed dominant peaks of V-D-J junction size in one or more Vβ families. Dominant peaks were seen most frequently in six Vβ families (Vβ7, 12, 13, 14, 16, and 23) and were never seen in seven others. Further analysis of the oligoclonal Vβ products showed dominant peaks in the J region as well. Of particular interest was the finding that Vβ and Jβ peaks were similar in inflamed metastases obtained at different times or from different sites from the same patient. Although 6 of 10 patients expressed HLA-A1, there was no common pattern of TCR rearrangements among them. Finally, the amplified PCR products from seven of these specimens were cloned and sequenced and the amino acid sequence of the complementarity-determining region 3 was deduced. In six of seven specimens, the same complementarity-determining region 3 sequence was repeated in at least two clones and in five of seven in at least three clones. Our study indicates that DNP vaccine induces the expansion of particular T cell clones that may be agents of its antitumor effects.

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“…Perhaps the most interesting aspect of Pd 2+ sensitization is, as noted above, its frequent, specific cross-sensitization with Ni 2+ [30][31][32]. The similarities in chemistry of Ni 2+ and Pd 2+ supports the idea of a similar mechanism involving common protein binding sites and conformational alterations [77]. While PdCl 2 and chloropalladates such as PdCl 4 2-and (NH 4 ) 2 PdCl 6 elicit a potent immune response in rodents, with occurrence of Pd-specific T cells [1], the incidence of sensitization within the human population probably reflects sensitization to Ni 2+ .…”

Section: Palladiummentioning

confidence: 68%

“…Therefore, recognition determinants on the T-cell receptor must also be important. Supporting this view, the Vβ region of the T-cell receptor appears to be involved, with a strong association between the Vβ phenotype and Ni 2+ sensitization; TCR-Vβ17 correlates with the extent of the T-cell response in vitro [77] and with the clinical severity of Ni 2+ -induced dermatitis [107]. Vollmer et al (107) have suggested that Ni 2+ may bridge the complementarity-determining region-1 of the T-cell receptor Vβ17 element and the MHC/peptide complex, enhancing otherwise weak receptor-peptide contacts.…”

Section: Mechanismsmentioning

confidence: 69%

Mechanisms of immunosensitization to metals (IUPAC Technical Report)

Templeton¹

2004

Pure and Applied Chemistry

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A project is underway within IUPAC to evaluate and harmonize the use of various biomarkers for immunosensitization to metals. This present review summarizes our knowledge of the mechanisms by which certain trace elements evoke allergenicity. Some physiological electrolytes (e.g., Na+, K+) and macronutrients (e.g., Ca2+, Fe3+) are immunologically inactive. However, some trace elements essential for cell function (e.g., Co2+, Cu2+, Cr3+), as well as nonessential elements generally considered toxic (e.g., Hg species) or in use as therapeutic agents (e.g., some species of Pt and Au), can give rise to adverse immune reactions. Specific immunological responses to Ni, Co, Cr, Hg, Be, Cu, Pt, Pd, Ir, In, and Au are discussed. In general, these elements can activate T or B cells by specific receptor interactions, resulting in clonal expansion of a metal-specific lymphocyte and an immune response (typically dermatitis) upon re-exposure. Compelling evidence points to the primary role of the T cell in responding to the metal. T-cell activation occurs when a protein of the major histocompatibility complex (MHC) binds to a T-cell receptor in the presence of an MHC binding peptide. Many antigenic substances result in presentation of MHC bound antigenic peptides to the T-cell receptor; metal ions appear to act as haptens that directly or indirectly cause structural changes in MHC molecule-peptide complexes that result in recognition of these complexes by specific T-cell subsets. Nickel and gold are particularly instructive in understanding mechanisms, and are used to discuss models in which the metal may bind to the antigenic peptide (i) before or (ii) after its association with the MHC/T-cell receptor complex, (iii) may bind to the MHC/receptor complex prior to recruitment of the antigenic peptide, or (iv) may bind to the formed peptide/MHC/receptor complex through ligands contributed by one or more of the components.

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Preferential Usage of TCR-Vβ17 by Peripheral and Cutaneous T Cells in Nickel-Induced Contact Dermatitis

Cited by 57 publications

References 31 publications

Diagnostic relevance of the lymphocyte transformation test for sensitization to beryllium and other metals (IUPAC Technical Report)

Diagnostic relevance of the lymphocyte transformation test for sensitization to beryllium and other metals (IUPAC Technical Report)

TCR Rearrangement in Lymphocytes Infiltrating Melanoma Metastases After Administration of Autologous Dinitrophenyl-Modified Vaccine

Mechanisms of immunosensitization to metals (IUPAC Technical Report)

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