Preferential translocation of boron to young leaves in<i>Arabidopsis thaliana</i>Regulated by the<i>BOR1</i>Gene

Takano, Junpei; Yamagami, Mutsumi; Noguchi, Kyotaro; Hayashi, Hiroaki; Fujiwara, Toru

doi:10.1080/00380768.2001.10408398

Cited by 91 publications

(86 citation statements)

References 32 publications

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“…Shoot growth in bor1-1 plants is severely inhibited under low-B conditions, but root growth is not (Takano et al, 2001). Borate RG-II cross linking in shoots is reduced in bor1-1 under the low-B condition, correlating with the reduced B concentrations in total shoots and shoot cell wall fraction (Noguchi et al, 2003).…”

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confidence: 96%

Roles of BOR2, a Boron Exporter, in Cross Linking of Rhamnogalacturonan II and Root Elongation under Boron Limitation in Arabidopsis

et al. 2013

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Boron (B) is required for cross linking of the pectic polysaccharide rhamnogalacturonan II (RG-II) and is consequently essential for the maintenance of cell wall structure. Arabidopsis (Arabidopsis thaliana) BOR1 is an efflux B transporter for xylem loading of B. Here, we describe the roles of BOR2, the most similar paralog of BOR1. BOR2 encodes an efflux B transporter localized in plasma membrane and is strongly expressed in lateral root caps and epidermis of elongation zones of roots. Transfer DNA insertion of BOR2 reduced root elongation by 68%, whereas the mutation in BOR1 reduced it by 32% under low B availability (0.1 mM), but the reduction in shoot growth was not as obvious as that in the BOR1 mutant. A double mutant of BOR1 and BOR2 exhibited much more severe growth defects in both roots and shoots under B-limited conditions than the corresponding single mutants. All single and double mutants grew normally under B-sufficient conditions. These results suggest that both BOR1 and BOR2 are required under B limitation and that their roles are, at least in part, different. The total B concentrations in roots of BOR2 mutants were not significantly different from those in wild-type plants, but the proportion of cross-linked RG-II was reduced under low B availability. Such a reduction in RG-II cross linking was not evident in roots of the BOR1 mutant. Thus, we propose that under B-limited conditions, transport of boric acid/borate by BOR2 from symplast to apoplast is required for effective cross linking of RG-II in cell wall and root cell elongation.

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confidence: 96%

Roles of BOR2, a Boron Exporter, in Cross Linking of Rhamnogalacturonan II and Root Elongation under Boron Limitation in Arabidopsis

et al. 2013

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“…Based on the observations that BOR1 stimulates xylem loading under B limitation and that the phenotype of bor1-1 can be easily reversed by high levels of B supply (8,9), it was expected that plants tightly control BOR1 activity with regard to external B availability.…”

Section: Bor1-mediated Xylem Loading Of B Is Regulated By B Availabilmentioning

confidence: 99%

“…bor1-1 mutant plants showed elevated sensitivity to B deficiency, especially in young growing organs in shoots, and the symptoms were associated with low-B contents in above-ground organs (8,9). Physiological studies showed that WT A. thaliana plants concentrated more B in the xylem sap under B-limiting conditions relative to bor1-1 mutant plants (10,11).…”

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confidence: 99%

Endocytosis and degradation of BOR1, a boron transporter ofArabidopsis thaliana, regulated by boron availability

Takano

Miwa

Yuan

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…For a long time, it has been believed that the distribution of B in the shoots is mainly determined by relative transpiration rather than demand (Shelp et al, 1995). However, on the other hand, it was observed that B was preferentially delivered to developing tissues in many plant species such as Arabidopsis (Takano et al, 2001), sunflower (Helianthus; Matoh and Ochiai, 2005), wheat (Triticum aestivum; Huang et al, 2001), and broccoli (Brassica oleracea, var. italica; Marentes et al, 1997), which have very low or no transpiration.…”

mentioning

confidence: 94%

“…organs for their active growth (Marentes et al, 1997;Takano et al, 2001;Huang et al, 2001;Matoh and Ochiai, 2005), but the molecular mechanism underlying this preferential distribution is poorly understood. In this study, we found that OsNIP3;1 localized in the nodes is required for this preferential distribution of B in rice.…”

Section: Silicon Transporter Lsi1 Largely Contributes To B Uptakementioning

confidence: 99%

Preferential Distribution of Boron to Developing Tissues Is Mediated by the Intrinsic Protein OsNIP3

et al. 2017

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Boron is especially required for the growth of meristem and reproductive organs, but the molecular mechanisms underlying the preferential distribution of B to these developing tissues are poorly understood. Here, we show evidence that a member of nodulin 26-like intrinsic protein (NIP), OsNIP3;1, is involved in this preferential distribution in rice (Oryza sativa). OsNIP3;1 was highly expressed in the nodes and its expression was up-regulated by B deficiency, but down-regulated by high B. OsNIP3;1 was polarly localized at the xylem parenchyma cells of enlarged vascular bundles of nodes facing toward the xylem vessels. Furthermore, this protein was rapidly degraded within a few hours in response to high B. Knockout of this gene hardly affected the uptake and root-to-shoot translocation of B, but altered B distribution in different organs in the above-ground parts, decreased distribution of B to the new leaves, and increased distribution to the old leaves. These results indicate that OsNIP3;1 located in the nodes is involved in the preferential distribution of B to the developing tissues by unloading B from the xylem in rice and that it is regulated at both transcriptional and protein level in response to external B level.

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Preferential translocation of boron to young leaves inArabidopsis thalianaRegulated by theBOR1Gene

Cited by 91 publications

References 32 publications

Roles of BOR2, a Boron Exporter, in Cross Linking of Rhamnogalacturonan II and Root Elongation under Boron Limitation in Arabidopsis

Roles of BOR2, a Boron Exporter, in Cross Linking of Rhamnogalacturonan II and Root Elongation under Boron Limitation in Arabidopsis

Endocytosis and degradation of BOR1, a boron transporter ofArabidopsis thaliana, regulated by boron availability

Preferential Distribution of Boron to Developing Tissues Is Mediated by the Intrinsic Protein OsNIP3

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