1985
DOI: 10.1016/0378-1097(85)90383-0
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Preferential synthesis, localisation and solubilisation of a precursor form of Bacillus subtilis levansucrase in an Escherichia coli minicell system

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1987
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1987
1987

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Cited by 1 publication
(2 citation statements)
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“…Thus, this strain seems to contain optimal characteristics for a biochemical study of levansucrase secretion.The existence of a 53000-M, precursor form of levansucrase was demonstrated by the expression of the cloned structural gene of this enzyme in a minicell-producing strain of Escherichia coli; the nucleotide sequence of the signal peptide was determined [5, 61. The levansucrase precursor form synthesized in minicells in the presence of phenethyl alcohol was found in the outer membrane fraction [7]. However, the yield of the precursor form accumulated in such conditions (less than 0.02% of total protein) was too low for purification.…”
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confidence: 98%
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“…Thus, this strain seems to contain optimal characteristics for a biochemical study of levansucrase secretion.The existence of a 53000-M, precursor form of levansucrase was demonstrated by the expression of the cloned structural gene of this enzyme in a minicell-producing strain of Escherichia coli; the nucleotide sequence of the signal peptide was determined [5, 61. The levansucrase precursor form synthesized in minicells in the presence of phenethyl alcohol was found in the outer membrane fraction [7]. However, the yield of the precursor form accumulated in such conditions (less than 0.02% of total protein) was too low for purification.…”
mentioning
confidence: 98%
“…The levansucrase precursor form synthesized in minicells in the presence of phenethyl alcohol was found in the outer membrane fraction [7]. However, the yield of the precursor form accumulated in such conditions (less than 0.02% of total protein) was too low for purification.…”
mentioning
confidence: 99%