Preferential loss of 5q14-21 in intestinal-type gastric cancer with DNA aneuploidy

Oga, Atsunori; Ishii, Yuriko; Izumi, Hideki; Park, Chang Young; Sasaki, Kohsuke

doi:10.1002/1097-0320(20010215)46:1<57::aid-cyto1038>3.0.co;2-5

Cited by 19 publications

(11 citation statements)

References 27 publications

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“…The chromosomal region of ATG10 (5q14) is frequently lost in ovarian cancer, gastric cancer, and breast cancer [19], [20]. However, recent genome-wide DNA microarray studies have revealed that the 5q14 region is amplified in neurofibrosarcoma and pancreatic cancer [21], [22].…”

Section: Discussionmentioning

confidence: 99%

Increased Expression of ATG10 in Colorectal Cancer Is Associated with Lymphovascular Invasion and Lymph Node Metastasis

Kim

Park

et al. 2012

PLoS ONE

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BackgroundAutophagy has paradoxical and complex functions in cancer development, and autophagy-related genes (ATG) are key regulators in autophagy. Until now, more than 30 different ATG proteins have been identified in yeast, and their mammalian counterparts also have been reported. Although the roles of a few ATG proteins in cancer have been characterized, the role of ATG10 is almost completely unknown.Methodology/Principal FindingsTo investigate the clinicopathological role of ATG10 in colorectal cancer, we analyzed ATG10 expression in colorectal cancer tissues and cell lines. Protein expression analysis showed that ATG10 is highly increased in colorectal cancer (tissue - 18/37 cases, 48%; cell line –8/12 cell lines, 66%). Immunohistochemical analysis with clinicopathological features indicated a strong association of the up-regulation of ATG10 with tumor lymph node metastasis (p = 0.005) and invasion (p<0.001). Moreover, both 5-year disease free survival and overall survival rates of patients bearing tumors that did not express ATG10 were significantly higher than those of patients bearing ATG10-expressing tumors (p = 0.012).Conclusion/SignificanceIncreased expression of ATG10 in colorectal cancer is associated with lymphovascular invasion and lymph node metastasis indicating that ATG10 may be a potential prognostic maker in colorectal cancer.

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Section: Discussionmentioning

confidence: 99%

Increased Expression of ATG10 in Colorectal Cancer Is Associated with Lymphovascular Invasion and Lymph Node Metastasis

Kim

Park

et al. 2012

PLoS ONE

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“…The specimens of the intestinal-type tumors were used in our previous report. 17) The tumors were located in the upper third of the stomach (2 cases), the middle third of the stomach (11 cases) or the lower third of the stomach (20 cases). All tumors were advanced cancer.…”

Section: Methodsmentioning

confidence: 99%

DNA Sequence Copy Number Aberrations Associated with Histological Subtypes and DNA Ploidy in Gastric Carcinoma

Oga

Park

Kawauchi

et al. 2001

Japanese Journal of Cancer Research

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We have analyzed DNA sequence copy number aberrations (DSCNAs) and DNA ploidy by using comparative genomic hybridization and laser scanning cytometer in gastric carcinomas (GCs) to elucidate the genomic aberrations in relation to clinicopathological parameters. Thirty-two out of 33 cases showed one or more DSCNAs with a mean number of 11.7 per tumor. High-level gains were detected at 2p, 3q, 6p, 7p, 7q, 8q, 12p, 13q, 19q, and 20q. Frequency of gross genomic abnormalities and chromosome regions that have genomic aberrations were similar in both intestinaland diffuse-type GCs, except aberrations at 8p, 9p, 12q, and 20q. The overall number of DSCNAs was significantly greater in DNA aneuploid tumors than that in DNA diploid tumors. We detected genomic aberrations characterized by histological subtype, tumor location, and DNA ploidy status: gain of 20q and losses of 8p and 9p in intestinal-type GCs, gains of 8p and 12q in diffuse-type GCs, gain of 20q in the lower third GCs, and loss of 5q, 9p, 10q, 16q, and 18q in DNA aneuploid GCs. Furthermore, 5q loss is associated with DNA aneuploidy (P = = = =0.0001) or the total number of losses (P = = = =0.001), gain + + + +losses (P = = = =0.004), and high-level gains (P = = = =0.001) in GCs. Among these loci, chromosome 8p was unique. Gain of 8p was more common in diffuse-type GC, whereas loss of 8p was more frequently detected in intestinal-type GC. In conclusion, we describe chromosomal regions of 5q, 8p, and 20q, which are of interest for further investigation of GCs.

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“…These include gains of 1q, 8q, 13q and 20q. In particular, the 20q region has been found to show gains (range 23–100%), and sometimes amplification, in a number of CGH studies on gastric adenocarcinoma 9, 10, 12–14, 16–21. Based on the chromosome CGH findings, the q12–13 region was considered to be the relevant area on chromosome 20 12.…”

Section: Introductionmentioning

confidence: 99%

Determination of amplicon boundaries at 20q13.2 in tissue samples of human gastric adenocarcinomas by high‐resolution microarray comparative genomic hybridization

Weiss

Snijders

Kuipers

et al. 2003

The Journal of Pathology

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Comparative genomic hybridization (CGH) of gastric adenocarcinomas frequently shows gains and amplifications of chromosome 20. However, the underlying genetic lesion is unknown and conventional CGH results do not allow specification of the target region. In order to investigate this chromosomal aberration with a higher resolution and sensitivity, microarray-based CGH was performed with both scanning and high-resolution arrays of chromosome 20 in a series of 27 gastric adenocarcinomas. Locus-specific fragments of genomic DNA from bacterial artificial chromosome (BAC) clones were spotted as microarrays. A scanning array contained a set of 27 BAC clones covering chromosome 20q. A high-resolution array contained 27 overlapping BAC clones at 20q13.2. This high-resolution array was used to narrow down the amplicon at 20q13.2 in tumours showing amplification of this chromosomal region with the scanning array. Positive copy number changes on chromosome 20q were detected in 12 of 27 cases (44%). These changes included gain of the whole arm of chromosome 20q in 8 of 27 (30%) cases, amplification restricted to 20q12.1 in one case, and amplifications restricted to 20q13 in three cases (11%). The three tumours showing amplification restricted to 20q13 were analysed further using the high-resolution array. In one tumour, the whole contig was amplified at a constant level. One of the other two tumours had a clear proximal breakpoint, while the other tumour had a clear distal breakpoint within the 20q13.2 region. The proximal and the distal breakpoint were approximately 800 kb apart. In the present study, an amplicon at 20q13.2 has been narrowed down to 800 kb which is likely to harbour one or more putative oncogenes relevant to gastric carcinogenesis, for which ZNF217 and CYP24 are good candidates.

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Preferential loss of 5q14-21 in intestinal-type gastric cancer with DNA aneuploidy

Cited by 19 publications

References 27 publications

Increased Expression of ATG10 in Colorectal Cancer Is Associated with Lymphovascular Invasion and Lymph Node Metastasis

Increased Expression of ATG10 in Colorectal Cancer Is Associated with Lymphovascular Invasion and Lymph Node Metastasis

DNA Sequence Copy Number Aberrations Associated with Histological Subtypes and DNA Ploidy in Gastric Carcinoma

Determination of amplicon boundaries at 20q13.2 in tissue samples of human gastric adenocarcinomas by high‐resolution microarray comparative genomic hybridization

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