Preferential hepatic uptake of iron from rat asialotransferrin: possible engagement of two receptors

Rudolph, John R.; Regoeczi, E.; Chindemi, Paul A.; Debanne, Maria T.

doi:10.1152/ajpgi.1986.251.3.g398

Cited by 18 publications

(19 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transferrin concentrations decrease rapidly in inflammation, apparently to deprive bacteria of iron needed for growth (30,31). Sialylation of transferrin influences its half-life and its function: desialylated transferrin is responsible for faster delivery of iron to hepatic storage (32) and also has a shorter half-life than normally sialylated transferrin (30); transferrin with a low sialic acid content also increases iron turnover and leads to iron loading of the hepatocyte (33).…”

Section: Discussionmentioning

confidence: 99%

Change of Transferrin Sialylation Differs between Mild Sepsis and Severe Sepsis and Septic Shock

Gornik

Kolednjak

et al. 2011

Intern. Med.

View full text Add to dashboard Cite

Objective and Design To investigate the association between the severity of sepsis and changes in sialylation of serum proteins we have conducted a single center pilot study. Subjects and Methods Sialylation of transferrin (with enzyme-linked lectin assay-ELLA) and total serum proteins (with colorimetric assay) as well as serum iron and transferrin levels were measured in 27 patients with sepsis through the first eight days of the disease. Results Total serum sialylation increased in the first two days, transferrin sialylation decreased, while serum iron and transferrin fell. Patients who developed severe sepsis had either a small or marked change in transferrin sialylation while in patients with mild sepsis sialylation decreased moderately. Conclusion We hypothesize that the change in transferrin sialylation could be a reflection of the intensity of inflammatory response which is insufficient if under-expressed and detrimental if over-expressed. This new feature is a potential marker of sepsis severity early in the disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Change of Transferrin Sialylation Differs between Mild Sepsis and Severe Sepsis and Septic Shock

Gornik

Kolednjak

et al. 2011

Intern. Med.

View full text Add to dashboard Cite

show abstract

“…Quantities of radioactivities injected were determined gravimetrically [13]. The protein doses per 100 g body wt were of the order of 10-20/2g for 125I-, 170-190/2g for 3H-and 90-100/2g for 14C-labelled preparations.…”

Section: Methodsmentioning

confidence: 99%

“…Rat transferrin (rTf) of the type that contains a standard biantennary glycan was isolated as in [13] from the plasmas of normal female SpragueDawley rats and from a rat which had received [3H]fucose. The tracer (500/~Ci, 55 Ci/mmol, ICN Radiochemicals, Irvine, CA) was injected i.p.…”

Section: Isolation and Preparation Of Tracer Proteinsmentioning

confidence: 99%

“…and the recipient exsanguinated 6 h later. Fucose-containing and fucose-free forms of rTf were separated by affinity chromatography on a column (1 x 5 cm) of lentil-Sepharose (Sigma, St. Louis, MO) as before [13]. Samples (50-100/2g) of either form of rTf were labelled with 125I (New England Nuclear, Boston, MA) by using tetrachloroglycoluril according to specifications given elsewhere [14].…”

Section: Isolation and Preparation Of Tracer Proteinsmentioning

confidence: 99%

See 1 more Smart Citation

Metabolic stability of the fucose in rat transferrin

Regoeczi

Chindemi

1987

FEBS Letters

View full text Add to dashboard Cite

The metabolic behaviour of the chitobiose core fucose that is a natural constituent of a large proportion of rat transferrin molecules was studied in rats comparatively to that of the polypeptide portion of the glycoprotein by using appropriate labels ([aH]fucose and 125I) and affinity chromatographic techniques (lentilSepharose). No evidence was obtained to suggest that this residue is cleaved from the glycan in significant amounts before removal of the entire glycoprotein for catabolism. Similarly, p4C]fucose linked to GlcNAc residues in the antennae of human asialotransferrin was being eliminated in pigeons at the same rate as the polypeptide itself. It is concluded that in spite of transferrin's exposure to the cellular milieu, the fate of its fucose is distinctly different from that of the same in plasma membrane glycoproteins.Fucose; Glycoprotein metabolism; Transferrin

show abstract

“…In addition, a relation between CDT and liver siderosis was suggested from animal studies, in view of higher iron uptake from desialylated transferrin by hepatocytes. 18,19 The aim of this study was to define whether patients' iron status influences CDT values and, in particular, whether iron excess or defect interfere with CDT, reducing its sensitivity and specificity as a marker of alcohol abuse. We measured CDT in alcohol abusers with and without iron overload and in nonabusers with iron deficiency and with iron overload caused by genetic hemochromatosis (GH).…”

mentioning

confidence: 99%

Carbohydrate-deficient transferrin, a sensitive marker of chronic alcohol abuse, is highly influenced by body iron

et al. 1999

View full text Add to dashboard Cite

Carbohydrate-deficient transferrin (CDT), a microheterogeneous form of serum transferrin (Tf), has been proposed as the most reliable marker of chronic alcohol consumption, although unexplained false-positive and -negative results have been reported. We investigated whether body iron influenced CDT serum levels by studying alcohol abusers with or without iron overload and nonabusers with iron deficiency or iron overload caused by genetic hemochromatosis (GH). In alcohol abusers, CDT was significantly lower in the presence of iron overload than in the absence (24.6 ؎ 16.5 U/L vs. 33.3 ؎ 11.7 U/L; P F .01), with false-negative results almost exclusively in patients with iron overload. Similarly, in nonabusers with GH, CDT was lower than in normal controls (9.6 ؎ 2.2 U/L vs. 15.7 ؎ 3.3 U/L; P F .0001), whereas, patients with iron deficiency anemia had significantly higher levels than controls (28.1 ؎ 5.8 U/L vs. 15.7 ؎ 3.3 U/L; P F .0001). In nonabusers, iron supplementation therapy significantly decreased CDT levels in patients with iron deficiency anemia (33.7 ؎ 6.6 U/L vs. 21.7 ؎ 5.2 U/L; P ‫؍‬ .0007), while iron-depletion treatment significantly increased CDT levels in patients with GH (9.7 ؎ 2.0 U/L vs. 14.7 ؎ 4.0 U/L; P ‫؍‬ .001). Alcohol abusers had a significant relationship between liver iron concentration (LIC) and the reciprocal of CDT (r ‫؍‬ .65; P F .0001), while in nonabusers, there was a significant correlation between Tf and CDT (r ‫؍‬ .72; P F .0001). In conclusion, CDT serum levels are markedly affected by the patient's iron status, with iron overload reducing its sensitivity in alcohol abusers and iron deficiency its specificity in nonabusers. CDT can be considered a reliable marker of alcohol abuse only when iron stores are normal. (HEPATOL-OGY 1999;29:658-663.)

show abstract

Preferential hepatic uptake of iron from rat asialotransferrin: possible engagement of two receptors

Cited by 18 publications

References 22 publications

Change of Transferrin Sialylation Differs between Mild Sepsis and Severe Sepsis and Septic Shock

Change of Transferrin Sialylation Differs between Mild Sepsis and Severe Sepsis and Septic Shock

Metabolic stability of the fucose in rat transferrin

Carbohydrate-deficient transferrin, a sensitive marker of chronic alcohol abuse, is highly influenced by body iron

Contact Info

Product

Resources

About