Preferential Expression of the cDNA Encoding the Proteasome Subunit during the Growth/Differentiation Transition ofDictyosteliumCells

Chae, Soo-Cheon; Maeda, Yasuo

doi:10.1006/bbrc.1998.8306

Cited by 10 publications

(3 citation statements)

References 23 publications

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“…In another experiment, 40 μL of washed cells (1 × 10 8 cells/mL) were plated on nitrocellulose filters supported by 1.5% non‐nutrient agar. These plates were followed by incubation at 22°C, as previously described (Chae & Maeda 1998a,b; Hirose et al. 2000).…”

Section: Methodsmentioning

confidence: 99%

Novel functions of ribosomal protein S6 in growth and differentiation of Dictyostelium cells

et al. 2009

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We have previously shown that in Dictyostelium cells a 32 kDa protein is rapidly and completely dephosphorylated in response to starvation that is essential for the initiation of differentiation (Akiyama & Maeda 1992). In the present work, this phosphoprotein was identified as a homologue (Dd-RPS6) of ribosomal protein S6 (RPS6) that is an essential member for protein synthesis. As expected, Dd-RPS6 seems to be absolutely required for cell survival, because we failed to obtain antisense-RNA mediated cells as well as Dd-rps6-null cells by homologous recombination in spite of many trials. In many kinds of cell lines, RPS6 is known to be located in the nucleus and cytosol, but Dd-RPS6 is predominantly located in the cell cortex with cytoskeletons, and in the contractile ring of just-dividing cells. In this connection, the overexpression of Dd-RPS6 greatly impairs cytokinesis during axenic shake-cultures in growth medium, resulting in the formation of multinucleate cells. Much severe impairment of cytokinesis was observed when Dd-RPS6-overexpressing cells (Dd-RPS6 OE cells) were incubated on a living Escherichia coli lawn. The initiation of differentiation triggered by starvation was also delayed in Dd-RPS6 OE cells. In addition, Dd-RPS6 OE cells exhibit defective differentiation into prespore cells and spores during late development. Thus, it is likely that the proper expression of Dd-RPS6 may be of importance for the normal progression of late differentiation as well as for the initiation of differentiation.

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Section: Methodsmentioning

confidence: 99%

Novel functions of ribosomal protein S6 in growth and differentiation of Dictyostelium cells

et al. 2009

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“…Dd-EF2-null (ef2-null) cells were selected and grown in PS-medium containing 10 µg/ml of blasticidin S. To allow cells to differentiate, cells were harvested at the exponential growth phase, washed twice in BSS (Bonner's salt solution) (Bonner, 1947) and allowed to settle either in a 24-well titer plate (Falcon #3047) or on 1.5% non-nutrient agar. This was followed by incubation at 22°C, as previously described (Chae and Maeda, 1998a;Chae and Maeda, 1998b;Inazu et al, 1999).…”

Section: Methodsmentioning

confidence: 99%

“…We have identified several genes (car1, caf1, quit3, dia1, dia2, dia3) that are specifically or predominantly expressed in response to differentiation of starved Ax-2 cells from the PSpoint and analyzed their functions (Abe and Maeda., 1994;Abe and Maeda., 1995;Okafuji et al, 1997;Itoh et al, 1998;Chae and Maeda, 1998a;Chae and Maeda, 1998b;Chae et al, 1998;Inazu et al, 1999;Hirose et al, 2000). We have also demonstrated that the phosphorylation levels of 90 kDa and 101 kDa phosphoproteins are specifically reduced during early cellular differentiation from the PS-point (Akiyama and Maeda, 1992).…”

Section: Introductionmentioning

confidence: 99%

Unexpected roles of aDictyosteliumhomologue of eukaryotic EF-2 in growth and differentiation

Watanabe

Sakurai

Amagai

et al. 2003

Journal of Cell Science

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EF-2 is believed to be indispensable for polypeptide chain elongation in protein synthesis and therefore for cell proliferation. Surprisingly, we could isolate ef2 null cells from Dictyostelium discoideum that exhibited almost normal growth and protein synthesis, which suggests that there is another molecule capable of compensating for EF-2 function. The knock-out of Dictyostelium EF-2 (Dd-EF2H; 101 kDa phosphoprotein)impairs cytokinesis, resulting in formation of multinucleate cells. The initiation of differentiation, including the acquisition of aggregation competence, was delayed in Dd-ef2 null cells compared with that in wild-type. By contrast, Dd-ef2 overexpression enhanced the progression of differentiation, thus indicating a positive involvement of Dd-EF2H in growth/differentiation transition.

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Transient expression of a mitochondrial gene cluster includingrps4 is essential for the phase-shift ofDictyostelium cells from growth to differentiation

1999

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Preferential Expression of the cDNA Encoding the Proteasome Subunit during the Growth/Differentiation Transition ofDictyosteliumCells

Cited by 10 publications

References 23 publications

Novel functions of ribosomal protein S6 in growth and differentiation of Dictyostelium cells

Novel functions of ribosomal protein S6 in growth and differentiation of Dictyostelium cells

Unexpected roles of aDictyosteliumhomologue of eukaryotic EF-2 in growth and differentiation

Transient expression of a mitochondrial gene cluster includingrps4 is essential for the phase-shift ofDictyostelium cells from growth to differentiation

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