Preferential adsorption of plasma proteins onto apolar polyurethane microdomains

Goodman, Steven L.; Simmons, Scott R.; Cooper, Stuart L.; Albrecht, Ralph M.

doi:10.1016/0021-9797(90)90129-c

Cited by 37 publications

(27 citation statements)

References 22 publications

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“…The water layer was separated and extracted twice with 50 mL of ether. The combined organic phases were washed with brine and deionized water, and then dried over MgSO 4 . The filtered solution was concentrated …”

Section: -Bromoundecylamine-hydrobromide (2)mentioning

confidence: 99%

“…1 Several studies have indicated that the interfacial interactions between the biological environment and the biomaterial are mediated by the surface properties of artificial material, such as surface hydrophilicity, 2 surface chemical composition, 3 surface microphase separation, 4 and surface charge. 5 Henceforth, to optimize the surface properties of the artificial biomaterial has become the major research theme in improving its biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

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Surface characterization and platelet adhesion studies for the mixed self‐assembled monolayers with amine and carboxylic acid terminated functionalities

Chuang

Lin

2007

J Biomedical Materials Res

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The mixed self-assembled monolayers (SAM) prepared from long chain alkanethiols, HS(CH(2))(11)NH(2) and HS(CH(2))(10)COOH, on gold are employed as the model surface for investigating the interactions between the biological environment and synthetic surface. A distinctive SAM preparation scheme was utilized in this investigation. The triethylamine was added to the alkanethiol solution during SAM formation and then followed by additional rinsing of SAM with 10% CH(3)COOH or 1% HCl ethanolic solution. The contact angle values of NH(2) + COOH mixed SAMs were between those of the pure SAMs, except that it was prepared with solution mole fraction of amine-terminated alkanethiol at 0.2. X-ray photoelectron spectroscopy (XPS) analysis has indicated that these two distinctive SAM preparation procedures had both resulted in a reduction in oxidized sulfur species on pure --NH(2) terminated SAM. However, the procedure utilizing 1% HCl ethanolic washing solution was more effective in reducing the unbound thiol fraction and to form a pure --NH(2) SAM with better quality. XPS analysis has also revealed that the surface of NH(2) + COOH mixed SAMs was "amine-rich". In vitro platelet adhesion assay has shown that the amount of adherent platelets on pure positive charged --NH(2) terminated SAM is less than that on anionic --COOH terminated counterpart in both acidic ethanolic washing schemes. Moreover, the lowest platelet adhesion density was noted on the mixed SAM surfaces with surface amine mole fraction at 0.51 and 0.57. This finding suggests that the surface charge with near neutrality might be of importance in reducing platelet adhesion and activation on artificial biomaterial.

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Section: -Bromoundecylamine-hydrobromide (2)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Surface characterization and platelet adhesion studies for the mixed self‐assembled monolayers with amine and carboxylic acid terminated functionalities

Chuang

Lin

2007

J Biomedical Materials Res

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“…[1,2] Nonspecific protein adsorption in biosensors can impair their proper function. Moreover, complications initially arising from protein adsorption to surfaces of, for example, catheters, [3,4] implants, [1,5] or artificial organs [3,6] introduced into the human body may lead to irritations, chronic infections, or thrombosis. [7,8] Despite the large impact of this problem on the above-mentioned applications and extensive research efforts, the details of the mechanism of nonspecific protein adsorption are not yet completely understood.…”

Section: Introductionmentioning

confidence: 99%

Self‐Assembled Monolayers of Dendritic Polyglycerol Derivatives on Gold That Resist the Adsorption of Proteins

Siegers

Biesalski

Haag

2004

Chemistry A European J

280

275

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Highly protein-resistant, self-assembled monolayers (SAMs) of dendritic polyglycerols (PGs) on gold can easily be obtained by simple chemical modification of these readily available polymers with a surface-active disulfide linker group. Several disulfide-functionalized PGs were synthesized by N,N'-dicyclohexylcarbodiimide-mediated ester coupling of thioctic acid. Monolayers of the disulfide-functionalized PG derivatives spontaneously form on a semitransparent gold surface and effectively prevent the adsorption of proteins, as demonstrated by surface plasmon resonance (SPR) kinetic measurements. A structure-activity relationship relating the polymer architecture to its ability to effectuate protein resistance has been derived from results of different surface characterization techniques (SPR, attenuated total reflectance infrared (ATR-IR), and contact-angle measurements). Dendritic PGs combine the characteristic structural features of several highly protein-resistant surfaces: a highly flexible aliphatic polyether, hydrophilic surface groups, and a highly branched architecture. PG monolayers are as protein resistant as poly(ethylene glycol) (PEG) SAMs and are significantly better than dextran-coated surfaces, which are currently used as the background for SPR spectroscopy. Due to the higher thermal and oxidative stability of the bulk PG as compared to the PEG and the easy accessibility of these materials, dendritic polyglycerols are novel and promising candidates as surface coatings for biomedical applications.

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“…Although it has been shown in the past that HSA preferentially adsorbs on non-polar, hydrophobic surfaces [36][37][38], there is also strong evidence that HSA will bind more readily and strongly to binding sites containing both hydrophobic and hydrophilic moieties and boundaries than either to hydrophobic solids or to surfaces with densely-packed hydrophobic molecules. Namely, it has been shown that the wetting transition zone of a carefully prepared wettability gradient surface will adsorb more HSA than more hydrophobic portions of the gradient [17,39].…”

Section: Discussionmentioning

confidence: 99%

Relating material surface heterogeneity to protein adsorption: the effect of annealing of micro-contact-printed OTS patterns

Hodgkinson¹,

Hlady²

2005

Journal of Adhesion Science and Technology

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We have investigated the influence of micrometer-and sub-micrometer-scale surface heterogeneities in patterned octadecyltrichlorosilane (OTS) films on human serum albumin (HSA) adsorption and its spatial distribution. 5-μm-wide OTS patterns were created on glass substrates by micro-contact printing and in some instances subsequent annealing was used to alter OTS molecule distribution within the patterns. Scanning force microscopy (SFM), advancing water contact angles and water vapor condensation figures were used to characterize the OTS films and to assess the nature of the heterogeneities within the various surface areas. High-resolution fluorescence microscopy was used to record images of fluorescently labeled albumin on OTS patterned films and fluorescence intensity was quantified and converted into the adsorbed amount. Adsorbed albumin was also characterized through SFM measurements. Combined SFM topography and lateral force microscopy (LFM) imaging revealed that micro-contact printing of OTS onto glass both replicated the stamp pattern and created small islands within the non-stamped regions between the patterns. The OTS coverage within stamped regions was not fully continuous but improved with subsequent annealing. Annealing also resulted in OTS island growth within the non-stamped regions and decreased average wettability on both the stamped and non-stamped areas. The extent of albumin adsorption was not proportional to OTS coverage, but correlated with the sub-μm distribution of OTS chains. We inferred that the surface distribution of ligands such as OTS on a sub-μm length scale determines the nature of albumin adsorption and its kinetics.

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Preferential adsorption of plasma proteins onto apolar polyurethane microdomains

Cited by 37 publications

References 22 publications

Surface characterization and platelet adhesion studies for the mixed self‐assembled monolayers with amine and carboxylic acid terminated functionalities

Surface characterization and platelet adhesion studies for the mixed self‐assembled monolayers with amine and carboxylic acid terminated functionalities

Self‐Assembled Monolayers of Dendritic Polyglycerol Derivatives on Gold That Resist the Adsorption of Proteins

Relating material surface heterogeneity to protein adsorption: the effect of annealing of micro-contact-printed OTS patterns

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