Objective: This study investigates the gut microbiome of Indians residing in India, Indian immigrants in Canada, and Canadians of Indian ancestry to understand the impact of westernization on their gut. Given the higher risk of Inflammatory Bowel Disease (IBD) among Indo-Canadians, these results provide insight into how westernization of the gut microbiome may influence incidence of IBD. Design: Stool samples were collected from healthy Indians in India, Immigrants born in India, Indo-Canadians, plus Canadian-borne individuals with European descent and Euro-Immigrants as non-Indian/westernized controls (N = 174).16S and shotgun sequencing compared microbial taxa and functional profiles across groups. Dietary and demographic data were collected to assess lifestyle patterns. Results: The microbiome of Indians and Indo-Immigrants were found to be distinct from westernized groups, with high abundances of Prevotella spp. and CAZymes known to facilitate the breakdown of plant-based foods, reflecting their traditional high complex carbohydrate diet. The Indian microbiome also showed characteristics of high bacterial cell turnover, pathogenic potential and stress tolerance, indicating their gut may be better primed to handle stressors on the gut. Indo-Canadians exhibited a transitional gut microbiome, harbouring taxa both common to Indians and westernized groups, which followed a pattern of increasing dietary acculturation. Conclusions: The transition from a non-westernized to westernized microbiome in Canadian-borne Indian migrants highlights the significant influence that the westernized lifestyle and dietary acculturation has on the gut microbiome. Considering globalization and immigration to westernized countries is occurring at a rapid rate, with 40.6% of the Canadian population made up of first- and second-generation immigrants,1 future research should investigate the health implications that such microbiome transitions have on immigrant populations and newly industrialized nations.