Preface

Roth, Henry Ling; Butler, Marion E.; Walker, James Backhouse; Garson, J. G.; Tylor, Edward B.

doi:10.1017/cbo9780511706189.001

Cited by 4 publications

(4 citation statements)

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“…The samples were read (100 ll) in a microplate reader at 630 nm every two hours. The doubling time (time required to double the biomass during the exponential growth) and growth rate (number of doublings per unit of time) were calculated from an exponential regression using the doubling time online calculator website (Roth, 2006).…”

Section: Methodsmentioning

confidence: 99%

Isolation and evaluation of autochthonous Bacillus subtilis strains as probiotics for fat snook (Centropomus parallelus Poey, 1860)

et al. 2016

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Summary The objective of this study was to isolate and select autochthonous strains of Bacillus subtilis from the fat snook, Centropomus parallelus, and examine the viability of the Bacillus bacteria to determine their beneficial effect on gut colonization in reared fish. Twenty strains of Bacillus were isolated and further confirmed as B. subtilis using PCR. Among the 20 strains, two strains (B02 and B03) exhibited an inhibitory performance against five tested pathogens. The Bacillus strains B02 and B03 were added to the fish ration, and after 30 days the number of viable colonies were maintained or increased under the conditions of refrigeration (5°C), freezing (−18°C), or room temperature (30°C). These strains showed a growth rate of 0.18–0.21 h and a doubling time of 3.34–3.9 h. Both strains were tolerant to variations in NaCl, and B03 was also tolerant to bile exposure. The ability to colonize the gastrointestinal tract was also examined in healthy fat snook juveniles fed diets enriched for 30 days with strains B02 and B03. The amount of B. subtilis in the gastrointestinal juvenile tract was significantly higher in fish fed the enriched ration compared to controls. Based on these results, strains B02 and B03 were considered as candidate probiotics for fat snook.

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Section: Methodsmentioning

confidence: 99%

Isolation and evaluation of autochthonous Bacillus subtilis strains as probiotics for fat snook (Centropomus parallelus Poey, 1860)

et al. 2016

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“…To assess any potential effect of cell labeling on cell viability or proliferation, we labeled cells (wild type and expressing luciferase) with SPIO (triplicate samples), harvested cells on different days, and counted the number of cells on an automatic cell counter. Doubling time was estimated assuming a exponential growth model: A t = A 0 2 t/td (where A t is the cell number at time t; A 0 is the cell number at time 0; t is time; t d is the doubling time) (36).…”

Section: Viability Assessment Of Labeled Cellsmentioning

confidence: 99%

In vivo MRI mapping of iron oxide‐labeled stem cells transplanted in the heart

Ruggiero

Guenoun

Smit

et al. 2013

Contrast Media & Molecular

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In various stem cell therapy approaches poor cell survival has been recognized as an important factor limiting therapeutic efficacy. Therefore noninvasive monitoring of cell fate is warranted for developing clinically effective stem cell therapy. In this study we investigated the use of voxel-based R₂ mapping as a tool to monitor the fate of iron oxide-labeled cells in the myocardium. Mesenchymal stem cells were transduced with the luciferase gene, labeled with ferumoxide particles and injected in the myocardium of healthy rats. Cell fate was monitored over a period of 8 weeks by bioluminescence and quantitative magnetic resonance imaging. Bioluminescence signal increased during the first week followed by a steep decrease to undetectable levels during the second week. MR imaging showed a sharp increase in R₂ values shortly after injection at the injection site, followed by a very gradual decrease of R₂ over a period of 8 weeks. No difference in the appearances on R₂-weighted images was observed between living and dead cells over the entire time period studied. No significant correlation between the bioluminescence optical data and R₂ values was observed and quantitative R₂ mapping appeared not suitable for the in vivo assessment of stem cell. These results do not follow previous in vitro reports where it was proposed that living cells may be distinguished from dead cells on the basis of the R₂ relaxivities (intracellular and extracellular iron oxides). Cell proliferation, cell migration, cell death, extracellular superparamagnetic iron oxide dispersion and aggregation exhibit different relaxivities. In vivo these processes happen simultaneously, making quantification very complex, if not impossible.

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“…"Without such behavior," the judge wrote with a distinct smirk, "the human race would soon disappear." 55 Even Frank's explanation for concurring in affirming Roth's conviction despite his obvious disdain for obscenity laws again nudged the Supreme Court to take the case, as he explained that he felt bound by the Court's seeming endorsement of such laws.…”

Section: Forging a Doctrinementioning

confidence: 99%

Slouching towardsRoth: Obscenity and the Supreme Court, 1945-1957

Strub¹

2013

Jnl of Supreme Court History

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Preface

Cited by 4 publications

References 0 publications

Isolation and evaluation of autochthonous Bacillus subtilis strains as probiotics for fat snook (Centropomus parallelus Poey, 1860)

Isolation and evaluation of autochthonous Bacillus subtilis strains as probiotics for fat snook (Centropomus parallelus Poey, 1860)

In vivo MRI mapping of iron oxide‐labeled stem cells transplanted in the heart

Slouching towardsRoth: Obscenity and the Supreme Court, 1945-1957

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