Preface to Special Topic: Selected Papers from the 2015 Annual Meeting of the AES Electrophoresis Society in Salt Lake City, Utah

Hughes, Michael

doi:10.1063/1.4954810

Cited by 1 publication

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References 12 publications

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“…The translation of polarized particles under a spatially nonuniform electric field was originally observed by Pohl 58 , and this dielectrophoresis behavior was observed to differ for live versus dead yeast cells 59,60,61 . The DEP force on a homogeneous spherical particle was solved as 62,63,64 : …”

Section: Microbial Polarizationmentioning

confidence: 82%

Review: Microbial analysis in dielectrophoretic microfluidic systems

Fernandez

Rohani

Farmehini

et al. 2017

Analytica Chimica Acta

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120

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Infections caused by various known and emerging pathogenic microorganisms, including antibiotic-resistant strains, are a major threat to global health and well-being. This highlights the urgent need for detection systems for microbial identification, quantification and characterization towards assessing infections, prescribing therapies and understanding the dynamic cellular modifications. Current state-of-the-art microbial detection systems exhibit a trade-off between sensitivity and assay time, which could be alleviated by selective and label-free microbial capture onto the sensor surface from dilute samples. AC electrokinetic methods, such as dielectrophoresis, enable frequency-selective capture of viable microbial cells and spores due to polarization based on their distinguishing size, shape and sub-cellular compositional characteristics, for downstream coupling to various detection modalities. Following elucidation of the polarization mechanisms that distinguish bacterial cells from each other, as well as from mammalian cells, this review compares the microfluidic platforms for dielectrophoretic manipulation of microbials and their coupling to various detection modalities, including immuno-capture, impedance measurement, Raman spectroscopy and nucleic acid amplification methods, as well as for phenotypic assessment of microbial viability and antibiotic susceptibility. Based on the urgent need within point-of-care diagnostics towards reducing assay times and enhancing capture of the target organism, as well as the emerging interest in isolating intact microbials based on their phenotype and subcellular features, we envision widespread adoption of these label-free and selective electrokinetic techniques.

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