N deficiency caused a decrease in leaf N content, Chlorophyll a and carbon assimilation of crop plants, resulting in a lower dry matter accumulation. It increases overall plant growth, but the nature of this response depends upon patterns of plant, nitrogen allocation, nutrient that vary throughout the growing season and depend upon canopy position. Decreased photosynthetic capacity is not only associated with direct effects of N deficiency but also with a negative feedback mechanism from the leaf carbohydrate pool. Because of the high requirement of crop plants for elemental N and its numerous important roles in growth and development, N is the mineral element that most often limits crop productivity. Because N mineralization from the soil is normally too low to support desired production levels, soil N levels are typically increased through fertilization. However, the complex cycle of N in the environment causes uncertainty in N fertilizer management, increasing the chances for economic loss and environmental damage. Nitrogen use and productivity of crop plants is also complex, resulting from an interaction of biochemical, physiological, and morphological processes in the plant. Application of N increases N content in the leaves of the soybean plant which increase growth rate, Leaf are, leaf area index and other physiology of the plant during the study.