Semperula maculata is most commonly found land slug in Vidarbha region and it is abundantly available in the field andgardens.It is an important species on earth. They play significant role in their ecosystem and serve humans in many ways. Acclimation refers usually to the compensatory change in an organism under maintained deviation of a single environmental factor (usually in the laboratory). Terrestrial animals are subjected too much greater fluctuation in the temperature and their body temperature is closely related to their water balance. Higher utilization of total lipid content in hepatopancreas and foot of the slug, Semperula maculata on warm acclimation (32°C and 36°C). Similarly catabolism and bioconversion of incorporated total lipid content in the hepatopancreas and foot was lowered down at cold acclimated (10°C and 15°C) than at warm acclimated temperature. The findings of total lipid content in the slug, Semperula maculata at cold and warm acclimated temperature suggests that the slug is capable of adapting changes in the environmental temperature by modifying content.