Preface

DNA-anti-DNA immune complexes. Antibody protection of a discrete DNA fragment from DNase digestion in vitro.

“…F(ab32 fragments were obtained by pepsin digestion of IgG at pH 4.2 for 16 h at 230C (15). Intact IgG was removed by passage over a SPA column and amino acids and pFc' were removed by gel filtration over Sephadex G50 (Pharmacia Fine Chemicals).…”

“…Intact IgG was removed by passage over a SPA column and amino acids and pFc' were removed by gel filtration over Sephadex G50 (Pharmacia Fine Chemicals). Fab' fragments were obtained by reduction and alkylation of F(ab')2 using 4.5 mM dithiothreitol for 20 min followed by 9 mM iodoacetamide for I h (15).…”

Coding strategy differences between constant and variable segments of immunoglobulin genes

“…This vertical separation is not understood and may be related to the paucity of the lambda segment library (the five murine lambda chain segments all come from two distinct genes); mouse lambda chains only account for 3 to 5% of total serum immunoglobulins (29). We now consider positioning of codons rather than segments.…”

In Memorium Alfred Nisonoff 1923–2001

“…A major occupation and preoccupation of the later Chicago years was writing, together with John Hopper and Susan Spring, the monograph, The Antibody Molecule, a monumental annotated work providing a scholarly and comprehensive review of the structural studies (1). Choosing to review the field at this time was a reflection of Nisonoff's astute insight, for it provided a definitive summary of the protein phase of molecular immunology and set the stage for the genetic era which was soon to follow.…”

“…In addition to fundamental work that helped to define the nature of antibodies and the genes encoding them, he was an astute critic with penetrating analytical skills. His monograph, The Antibody Molecule (1), still stands as the definitive reference work on the subject to 1975, the time of its publication.…”