Preemptive analgesic effects of midazolam and diclofenac in rat model

Hasani, Antigona; Soljakova, Marija; Jakupi, M; Ustalar-Ozgen, Serpil

doi:10.17305/bjbms.2011.2593

Cited by 20 publications

(13 citation statements)

References 30 publications

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“…In this context, Yano and colleagues (2006) demonstrated that, in mice, methyleugenol (10 µg/kg) isolated from Asiasari radix significantly diminished the duration of paw licking and biting time in phase 2 of the formalin test without affecting phase 1. This behavior is similar to that found in our experiments for eugenol and comparable to that produced by diclofenac, which itself has demonstrated an effect in the formalin test (Picazo, Castañeda-Hernández, Ortiz, 2006) and the thermal model of inflammation (Hasani et al, 2011).…”

Section: Discussionsupporting

confidence: 89%

Antinociceptive local activity of 4-allyl-1-hydroxy-2-methoxybenzene (eugenol) by the formalin test: an anti-inflammatory effect

Lugo-Lugo

Pozos‐Guillén

Zapata-Morales

et al. 2019

Braz. J. Pharm. Sci.

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Eugenol has been employed for decades as a condiment, an antimycotic, an antibacterial, an antiviral, and an antioxidant, and it is one of the natural analgesics most frequently utilized for pain and inflammation. Our objective was to determine the analgesic/anti-inflammatory effect of eugenol compared with diclofenac, naproxen, and tramadol using the formalin test. The formalin method was used in 6-to 10-week-old Wistar rats (weighing 250 g each) divided into six groups: saline (0.9%); formalin (5%); diclofenac (250 µg/kg); naproxen (400 µg/kg); tramadol (500 µg/kg), and eugenol (1,400 µg/kg), in the intraplantar part of the hind-end trunk of the rats, with n = 5 per group. Eugenol diminished 44.4% of nociceptive behavior in phase 1 and 48% in phase 2 (p ≤0.05 vs formalin). Eugenol was shown to be 1.14 times more effective than diclofenac, but 1.62 and 1.75 times less effective than naproxen and tramadol, respectively, in phase 1 and 1.45 times less effective than diclofenac and naproxen and 1.66 less effective than tramadol in phase 2 (p ≤0.05). These data suggest that eugenol possesses moderate activity in the acute pain phase and greater activity in inflammatory-type pain, and both effects are comparable to those produced by diclofenac and are less than the effects produced by naproxen and tramadol in the formalin test.

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Section: Discussionsupporting

confidence: 89%

Antinociceptive local activity of 4-allyl-1-hydroxy-2-methoxybenzene (eugenol) by the formalin test: an anti-inflammatory effect

Lugo-Lugo

Pozos‐Guillén

Zapata-Morales

et al. 2019

Braz. J. Pharm. Sci.

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“…The doses were based on previous toxicity studies and within the normal range for testing edible plant extracts in rodent pre‐clinical studies (Mohamad Shalan et al, ). The Diclofenac dose is based on the recommended dose for rats (Hasani, Soljakova, Jakupi, & Ustalar‐Ozgen, ). Osteoarthritis (OA) were induced by intra‐articular injection of monosodium iodoacetate (MIA 3 mg in 50 μl saline) into the right knee joint (Wan Osman et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…The Shalan et al, 2017). The Diclofenac dose is based on the recommended dose for rats (Hasani, Soljakova, Jakupi, & Ustalar-Ozgen, 2011). Osteoarthritis (OA) were induced by intra-articular injection of monosodium iodoacetate (MIA 3 mg in 50 μl saline) into the right knee joint (Wan Osman et al, 2017).…”

Section: Animal Study For Osteoarthritismentioning

confidence: 99%

Epicatechin and scopoletin richMorinda citrifolia(Noni) leaf extract supplementation, mitigated Osteoarthritis via anti-inflammatory, anti-oxidative, and anti-protease pathways

et al. 2019

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The scopoletin (coumarin) and epicatechin (flavonoid) rich Morinda citrifolia L. (MC) Noni leaves are non‐toxic (unlike the fruits) and consumed as vegetables. The anti‐osteoarthritis effects of the MC leaf extract against joint cartilage degradation and inflammation were investigated through cartilage explant cultures and pre‐clinical animal study. Osteoarthritis were induced by intra‐articular monosodium iodoacetate injection into the right knee. The extract, scopoletin and epicatechin, suppressed glycosaminoglycan and nitric oxide release from the cartilage explant in the presence of Interleukin‐1β. After 28 days, the extract treatment reduced the in vivo serum levels and joint tissues mRNA expressions for joint cartilage degradation, aggrecanase, and collagenase biomarkers. The extract increased the bone formation marker PINP levels, besides improving the articular cartilage structure and chondrocytes cellularity. The extract improved bone formation/repair, subchondral bone structure, strength and integrity, as well as cartilage synthesis by suppressing inflammation, nitric oxide production, joint catabolism by proteases, and oxidative stress. Practical applications The scopoletin (coumarin) and epicatechin (flavonoid) rich Morinda citrifolia (Noni) leaves may be used as vegetables, functional food ingredient, or dietary supplements to suppress osteoarthritis progression against joint cartilage degradation and inflammation. The extract, scopoletin, or epicatechin, suppressed glycosaminoglycan, and nitric oxide release from the cartilage. The Morinda citrifolia leaf extract suppressed inflammation, nitric oxide production, tissues catabolism by proteases and oxidative stress to help reduce joint cartilage degradation, besides improving the articular cartilage structure, chondrocytes health, subchondral bone structure, bone formation/repair, and cartilage synthesis.

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“…The Diclofenac dose is based on the reported recommended dose for rats (Hasani, Soljakova, Jakupi, & Ustalar-Ozgen, 2011). Diclofenac is the Food and Drug Administration approved drug (non-steroidal anti-inflammatory drug) for OA.…”

Section: Animal and Groupingmentioning

confidence: 99%

Scopoletin‐standardized Morinda elliptica leaf extract suppressed inflammation and cartilage degradation to alleviate osteoarthritis: A preclinical study

Osman

Lau

Mohamed

2017

Phytotherapy Research

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The effect of scopoletin-standardized Morinda elliptica leaf extract against osteoarthritis was investigated in ex vivo explant culture and preclinical rodent model. Thirty male rats were grouped (n = 6) into untreated osteoarthritis (OA), OA + Diclofenac (5 mg/kg), and OA + extract (200 and 400 mg/kg) and compared with healthy control. Monosodium iodoacetate were injected into the right intra-articular knee joints to induce OA. The rats were evaluated for OA severity via physical (micro-CT and histological observations), biochemical, ELISA, and mRNA expression analysis (for inflammation and cartilage degradation biomarkers), after 28 days of treatment. The extract suppressed glycosaminoglycan release from the cartilage explant in the presence of Interleukin-1β. The 200 mg/kg dose appeared better than 400 mg/kg dose, at reducing cartilage and subchondral bone erosions in OA-induced rats, by significantly down-regulating the collagenases and aggrecanase. The extract dose-dependently reduced serum inflammation biomarkers and increased bone formation biomarkers to near normal levels in the OA-induced rats. M. elliptica leaf scopoletin-standardised extract alleviated OA progression and articular cartilage structure, by ameliorating cartilage degradation, nitric oxide levels, inflammation, bone /cartilage homeostasis, collagenase/aggrecanase activities, chondrocytes survival, subchondral bone structure and integrity.

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Preemptive analgesic effects of midazolam and diclofenac in rat model

Cited by 20 publications

References 30 publications

Antinociceptive local activity of 4-allyl-1-hydroxy-2-methoxybenzene (eugenol) by the formalin test: an anti-inflammatory effect

Antinociceptive local activity of 4-allyl-1-hydroxy-2-methoxybenzene (eugenol) by the formalin test: an anti-inflammatory effect

Epicatechin and scopoletin richMorinda citrifolia(Noni) leaf extract supplementation, mitigated Osteoarthritis via anti-inflammatory, anti-oxidative, and anti-protease pathways

Scopoletin‐standardized Morinda elliptica leaf extract suppressed inflammation and cartilage degradation to alleviate osteoarthritis: A preclinical study

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