Predominant Tuberculosis Spoligotypes, Delhi, India

Abstract

One hundred five Mycobacterium tuberculosis clinical isolates from the Delhi area were typed by spoligotyping; 45 patterns were identified. Comparison with an international spoligotype database showed type 26, Delhi type (22%), type 54 (12%), and type 1, Beijing type (8%), as the most common. Eighteen spoligotypes did not match any existing database pattern.

Comparison of spoligotyping, mycobacterial interspersed repetitive units typing and IS6110-RFLP in a study of genotypic diversity of Mycobacterium tuberculosis in Delhi, North India

“…Although various studies have been performed in India using spoligotyping of M. tuberculosis (Mistry et al 2002, Singh et al 2004, Kulkarni et al 2005, Mathuria et al 2008), very few studies have reported the use of MIRU-VNTRs for molecular typing of M. tuberculosis isolates , Stavrum et al 2009, Narayanan et al 2010. Therefore, the present study validates the use of repetitive elements for molecular typing of M. tuberculosis in Indian isolates and further underlines the usefulness of MIRU-VNTRs for discriminating low-IS6110-copy isolates, which accounted for more than one-fifth of the strains in the present study.…”

“…Furthermore, IS6110 typing is labour intensive and requires several weeks for culturing the M. tuberculosis isolates. Fingerprinting methods targeting polymorphic spacer sequences in the direct repeat (DR) region, including spoligotyping, have been used in some regions in India (Mistry et al 2002, Singh et al 2004, Kulkarni et al 2005, Mathuria et al 2008, Narayanan et al 2008. However, when used alone, these methods may underestimate the clonal diversity of M. tuberculosis (Kremer et al 1999).…”

Genomic interrogation of ancestral Mycobacterium tuberculosis from south India

“…The CAS or Delhi type corresponding to ST26 which predominates in North India (Bhanu et al, 2002, Singh et al, 2004, Kulkarni et al, 2005, Guitierrez et al, 2006) represents 1.02 % of isolates SpolDB4 database. This type has been reported from 34 countries.…”

“…Although one fourth of the global tuberculosis burden stems from India there are a few studies which define the tuberculosis genogroup (Bhanu et al, 2002, Das, et al, 2005, Mistry et al, 2002, Narayanan et al, 1997, Kulkarni et al, 2005, Singh et al, 2004, Radhakrishnan et al, 2001. There has been a population based study from south India on transmission dynamics and risk factors associated with transmission (Narayanan, et al, 2002).…”

Identifying Mycobacterium tuberculosis complex strain families using spoligotypes

“…We kept the M. bovis-BCG name for the family to stay consistent with the SpolDB3-based notation (Filliol et al, 2002). (Singh et al, 2004). Family33 appeared to gather spoligotypes with most of the spacers present and that could not "find" any parent other than the putative, given the current hypothesis of the evolution of the DR locus by deletion of spacers, common ancestor, which was a prototype for this family.…”