Predominant role of the l-galactose pathway in l-ascorbic acid biosynthesis in fruits and leaves of the Capsicum annuum L. chili pepper

Gómez-García, María del Rocío; Ochoa-Alejo, Neftalí

doi:10.1007/s40415-015-0232-0

Cited by 18 publications

(26 citation statements)

References 48 publications

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“…In this work, we calibrated the gene expression at each fruit stage, also to avoid possible confounding factors related to the variability in expression of housekeeping genes at different developmental and maturity stages. The comparative analysis implies that transcriptional regulation of the AsA biosynthesis, especially for genes of the L-galactose pathway, is a relevant factor for the differential accumulation in our pepper varieties [25,39,41]. GGP and GGP/VCT2 were overexpressed in PEP1 during the whole maturation process, consistent with their key role in regulating the AsA pool content reported in different plant species [22,41].…”

Section: Discussionsupporting

confidence: 64%

“…A more comprehensive understanding of all the genes involved in AsA metabolism could be addressed exploiting the power of NGS technologies. While the dynamic of the gene expression during maturation has been previously investigated [34,39], our aim was to compare behavior of the genes in the two contrasting varieties. Their relative gene expression analysis indicated that the increasing differences in AsA concentration during ripening are associated with a higher induction of genes involved in AsA biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptional Regulation of Ascorbic Acid During Fruit Ripening in Pepper (Capsicum annuum) Varieties with Low and High Antioxidants Content

Chiaiese

Corrado

Minutolo

et al. 2019

Plants

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Research on plant antioxidants, such as ascorbic acid (AsA) and polyphenols, is of increasing interest in plant science because of the health benefits and preventive role in chronic diseases of these natural compounds. Pepper (Capiscum annuum L.) is a major dietary source of antioxidants, especially AsA. Although considerable advance has been made, our understanding of AsA biosynthesis and its regulation in higher plants is not yet exhaustive. For instance, while it is accepted that AsA content in cells is regulated at different levels (e.g., transcriptional and post-transcriptional), their relative prominence is not fully understood. In this work, we identified and studied two pepper varieties with low and high levels of AsA to shed light on the transcriptional mechanisms that can account for the observed phenotypes. We quantified AsA and polyphenols in leaves and during fruit maturation, and concurrently, we analyzed the transcription of 14 genes involved in AsA biosynthesis, degradation, and recycling. The differential transcriptional analysis indicated that the higher expression of genes involved in AsA accumulation is a likely explanation for the observed differences in fruits. This was also supported by the identification of gene-metabolite relations, which deserve further investigation. Our results provide new insights into AsA differential accumulation in pepper varieties and highlight the phenotypic diversity in local germplasm, a knowledge that may ultimately contribute to the increased level of health-related phytochemicals.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Transcriptional Regulation of Ascorbic Acid During Fruit Ripening in Pepper (Capsicum annuum) Varieties with Low and High Antioxidants Content

Chiaiese

Corrado

Minutolo

et al. 2019

Plants

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“…The L-ascorbic acid (vitamin C) is the most abundant antioxidant in plant cells, which participates in diverse biological processes, including cell expansion, environment response, photoprotection, and photosynthesis, etc. [ 78 ]. GLDH is responsible for the last step in producing vitamin C through the L-galactose pathway, and it was used here as a reference gene to find CaMYB genes candidates possibly involved in the control of vitamin C biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

“…GLDH is responsible for the last step in producing vitamin C through the L-galactose pathway, and it was used here as a reference gene to find CaMYB genes candidates possibly involved in the control of vitamin C biosynthesis. The CaMYB16 expression profile correlated positively with both the GLDH expression pattern and content of vitamin C throughout the chili pepper fruit development [ 78 ]. The constant production of vitamin C during the chili pepper fruit development can be necessary either for protection purposes or development.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Analysis of the MYB Transcription Factor Gene Family in Chili Pepper (Capsicum spp.)

Arce-Rodríguez

Martínez

Ochoa-Alejo

2021

IJMS

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The MYB transcription factor family is very large and functionally diverse in plants, however, only a few members of this family have been reported and characterized in chili pepper (Capsicum spp.). In the present study, we performed genome-wide analyses of the MYB family in Capsicum annuum, including phylogenetic relationships, conserved domain, gene structure organization, motif protein arrangement, chromosome distribution, chemical properties predictions, RNA-seq expression, and RT-qPCR expression assays. A total of 235 non-redundant MYB proteins were identified from C. annuum, including R2R3-MYB, 3R-MYB, atypical MYB, and MYB-related subclasses. The sequence analysis of CaMYBs compared with other plant MYB proteins revealed gene conservation, but also potential specialized genes. Tissue-specific expression profiles showed that CaMYB genes were differentially expressed, suggesting that they are functionally divergent. Furthermore, the integration of our data allowed us to propose strong CaMYBs candidates to be regulating phenylpropanoid, lignin, capsaicinoid, carotenoid, and vitamin C biosynthesis, providing new insights into the role of MYB transcription factors in secondary metabolism. This study adds valuable knowledge about the functions of CaMYB genes in various processes in the Capsicum genus.

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“…Among these species, C. annuum is the most important worldwide as a vegetable and spice crop, and production of this type of chili pepper has been steadily increasing in terms of both area harvested and yield [ 2 ]. In addition to their economic importance, chili peppers are a source of antioxidants, such as flavonoids, phenolic acids, carotenoids, and vitamins [ 3 , 4 ], as well as a plant model for studying the genetic and biochemical basis for the synthesis of these compounds [ 5 , 6 , 7 ]. Capsaicinoids, which are synthesized only in Capsicum species, impart pungency to chili peppers and are a focus of active research [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analyses Throughout Chili Pepper Fruit Development Reveal Novel Insights into the Domestication Process

Martínez

Arce-Rodríguez

Hernández-Godínez

et al. 2021

Plants

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Chili pepper (Capsicum spp.) is an important crop, as well as a model for fruit development studies and domestication. Here, we performed a time-course experiment to estimate standardized gene expression profiles with respect to fruit development for six domesticated and four wild chili pepper ancestors. We sampled the transcriptomes every 10 days from flowering to fruit maturity, and found that the mean standardized expression profiles for domesticated and wild accessions significantly differed. The mean standardized expression was higher and peaked earlier for domesticated vs. wild genotypes, particularly for genes involved in the cell cycle that ultimately control fruit size. We postulate that these gene expression changes are driven by selection pressures during domestication and show a robust network of cell cycle genes with a time shift in expression, which explains some of the differences between domesticated and wild phenotypes.

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Predominant role of the l-galactose pathway in l-ascorbic acid biosynthesis in fruits and leaves of the Capsicum annuum L. chili pepper

Cited by 18 publications

References 48 publications

Transcriptional Regulation of Ascorbic Acid During Fruit Ripening in Pepper (Capsicum annuum) Varieties with Low and High Antioxidants Content

Transcriptional Regulation of Ascorbic Acid During Fruit Ripening in Pepper (Capsicum annuum) Varieties with Low and High Antioxidants Content

Genome-Wide Identification and Analysis of the MYB Transcription Factor Gene Family in Chili Pepper (Capsicum spp.)

Transcriptome Analyses Throughout Chili Pepper Fruit Development Reveal Novel Insights into the Domestication Process

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