Predominant microflora associated with human dental periapical abscesses

Oguntebi, Bamiduro R.; Slee, Andrew M.; Tanzer, Jason M.; Langeland, Kaare

doi:10.1128/jcm.15.5.964-966.1982

Cited by 76 publications

(47 citation statements)

References 20 publications

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“…aeruginosa and possibly S. epidermidis, the demonstrated bacteria occur in the oral cavity on the oral mucosa, the gingiva, the dorsum of the tongue, or the tonsils (32,33). The bacteria have also been demonstrated in the root canals of teeth with necrotic pulps (1,22,34), in deep periodontal pockets (35)(36)(37), and in acute abscesses of periodontal (26,38,39) or endodontic origin (23)(24)(25)(26). This study demonstrated these organisms in asymptomatic periapical lesions, and since several of the organisms are considered endodontic pathogens, it seems reasonable to hypothesize that they indeed caused or at least significantly contributed to the lesions.…”

Section: Discussionsupporting

confidence: 57%

“…Invasive capacity has been demonstrated for black-pigmented Bacteroides species (15)(16)(17), Actinobacillus actinomycetemcomilans (18,19), Capnocytophaga (20) and a number of other bacteria (21). The fact that several of these organisms have been implicated in some form of periodontal disease may indicate that invasiveness is an important aspect ofthe virulence of periodontopathic bacteria . These microorganisms have been demonstrated in infected root canals (1,2,22) as well as in pus from abseesses of endodontic origin (23)(24)(25)(26). Therefore, it was the purpose ofthe present investigation to examine patients with asymptomatie periapical inflammatory lesions that were refractory to endo-dontic treatment for extraradicular endodontic infections.…”

mentioning

confidence: 99%

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Extraradicular endodontic infections

Tronstad

Barnett

Riso

et al. 1987

Dental Traumatology

204

167

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Eight asymptomatic periapical inflammatory lesions which were refractory to conventional endodontic therapy were examined for the presence of bacteria. Access to the periapical lesions was gained using an aseptic surgical technique. Sterile currettes were used to take microbiological samples from the soft tissue lesions and scrapings from the surface of the root tips. Möller's VMGA III transport medium was utilized and the bacteriological samples were processed using a continuous anaerobic technique. Bacterial growth was evident in the samples from all 8 lesions. Two lesions exclusively yielded anaerobic bacteria and 5 lesions were heavily dominated by anaerobes. Black‐pigmented Bacteroides species as well as anaerobic gram–positive rods and cocci were isolated. Staphylococcus epidermidis was recovered from 3 lesions and 2 Actinomyces species were isolated from 1 lesion. Facultative Streptococcus species were rare periapical isolates. Our study clearly showed that anaerobic bacteria are able to survive and maintain an infectious disease process in periapical tissues.

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Section: Discussionsupporting

confidence: 57%

mentioning

confidence: 99%

Extraradicular endodontic infections

Tronstad

Barnett

Riso

et al. 1987

Dental Traumatology

204

167

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“…Because endodontic infections are polymicrobial, the detection and identification of all associated organisms have been difficult and produced a great deal of confusing data. No absolute correlation has been made between any specific microbial species or combination of species with clinical signs and symptoms (14,(16)(17)(18)(19)(37)(38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)(54)(55).…”

Section: Sensitivity and Specificitymentioning

confidence: 99%

Microbiological and molecular analysis of endodontic infections

Baumgartner¹

2004

Endodontic Topics

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This article reviews microbiological and molecular methods used to study the microorganisms associated with endodontic infections. Over 500 species of bacteria have been cultivated from the oral cavity. Endodontic infections are polymicrobial with usually from 3–12 species cultivable from either infected root canals or periradicular abscesses. Molecular methods are able to detect and identify many additional species of bacteria associated with endodontic infections. Molecular methods provide precise identification of the microbes at the DNA level and detection of microbes that are not cultivable. Future research will detect and identify other as yet unknown species of bacteria, viruses, and fungi involved in endodontic infections. Which microorganisms produce virulence factors associated with serious endodontic infections will also be better defined using molecular methods. An increase in knowledge and understanding of the organisms associated with endodontic infections will improve our ability to clinically manage endodontic infections.

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“…Studies involving clinical, radiographic, histopathologic, and microbiologic aspects ofthe periapical lesion have been described extensively in the dental literature (1)(2)(3)(4)(5)(6)(7)(8)(9). It has also been shown that the immune system plays an important role (7,8).…”

mentioning

confidence: 99%

“…Although identified the bacteria in the canal that could be cultured, the localization of these bacteria in dentin was not demonstrated. Furthermore, it has been shown that culture and identification of bacteria in the periapical lesion is a difficult task (6). This is a very important gap m knowledge and should be investigated, because It has been demonstrated that in the carious breakdown of the pulp, micro-organisms invade the dentinal tubules of the root canal as far apically as necrosis is present (1).…”

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confidence: 99%

Development of predictable periapical lesion monitored by subtraction radiography

Pascon

Introcaso

Langeland

1987

Dental Traumatology

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Forty‐eight teeth of two baboons were used to establish a methodology for the development of predictable periapical lesions. The canines and third molars were used as controls. All pre‐ and post‐operative radiographs were taken using modified Precision® X‐ray beam guiding to maintain exposure geometry. After routine endodontic access preparation, the pulps were damaged 3–4 mm short of the apex, dental plaque was introduced and mixed with pulp debris, and the access opening was closed with amalgam. Control radiographs were taken at 7‐day intervals. Ten experimental and two control teeth were used for each observation period of 1, 4, 8, and 20 weeks. The radiographs were analyzed using computerized temporal subtraction. After the animals were killed, the specimens were processed for histologic examination. In all cases, pulpal necrosis and bacteria were present in the root canal, and in the periapical area adjacent to necrotic tissue. The periapical lesions had a mixture of inflammatory cells, with predominance of plasma cells, lymphocytes and multinucleated cells. Racliographic analysis by subtraction radiography showed hard tissue changes as early as 7 days. The method predictably developed periapical lesions, which could be monitored by subtraction radiography, and there was a correlation established between radiographic and histologic findings.

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Predominant microflora associated with human dental periapical abscesses

Cited by 76 publications

References 20 publications

Extraradicular endodontic infections

Extraradicular endodontic infections

Microbiological and molecular analysis of endodontic infections

Development of predictable periapical lesion monitored by subtraction radiography

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