Predominant gut Lactobacillus murinus strain mediates anti-inflammaging effects in calorie-restricted mice

Abstract

BackgroundCalorie restriction (CR), which has a potent anti-inflammaging effect, has been demonstrated to induce dramatic changes in the gut microbiota. Whether the modulated gut microbiota contributes to the attenuation of inflammation during CR is unknown, as are the members of the microbial community that may be key mediators of this process.ResultsHere, we report that a unique Lactobacillus-predominated microbial community was rapidly attained in mice within 2 weeks of CR, which decreased the levels of circulating microbial antigens and systemic inflammatory markers such as tumour necrosis factor alpha (TNF-α). Lactobacillus murinus CR147, an isolate in the most abundant operational taxonomic unit (OTU) enriched by CR, downregulated interleukin-8 production in TNF-α-stimulated Caco-2 cells and significantly increased the lifespan and the brood size of the nematode Caenorhabditis elegans. In gnotobiotic mice colonized with the gut microbiota from old mice, this strain decreased their intestinal permeability and serum endotoxin load, consequently attenuating the inflammation induced by the old microbiota.ConclusionsOur study demonstrated that a strain of Lactobacillus murinus was promoted in CR mice and causatively contributed to the attenuation of ageing-associated inflammation.Electronic supplementary materialThe online version of this article (10.1186/s40168-018-0440-5) contains supplementary material, which is available to authorized users.

Chronic water insufficiency induced kidney damage and energy dysregulation despite reduced food intake, which improved gut microbiota in female rats

“…The present study also showed that WAFR and WRFA reduced Firmicutes and increased Bacteroidetes regardless of water intake. However, in the present study, WAFR, not WRFA, increased Lactobacillales, which was comparable to the results of Pan et al [41]. However, no study has investigated how the gut microbiome is modulated by water restriction.…”

Lonicera caerulea L. Polyphenols Alleviate Oxidative Stress‐Induced Intestinal Environment Imbalance and Lipopolysaccharide‐Induced Liver Injury in HFD‐Fed Rats by Regulating the Nrf2/HO‐1/NQO1 and MAPK Pathways

“…[ 69 ] Lactobacillales , Lactobacillaceae , and Lactobacillus , which belong to the class Bacilli , also exert these functional effects. Recent studies have demonstrated that common prebiotics, such as Lactobacillus can reduce the levels of the systemic inflammation marker (TNF‐α), [ 70 ] Lactobacillaceae can participate in the microbial anti‐oxidation metabolic pathway, [ 71 ] and Lactobacillales can play an important role in maintaining the levels of the intestinal microbiome and SCFAs. [ 72 ] In this study, the correlation analysis indicated that Bacilli , Lactobacillales , Lactobacillaceae , and Lactobacillus can reduce LPS transportation and liver damage by restoring the intestinal barrier functions.…”

SEBS colonized in ileal mucosa, optimized bacterial composition, improved Se level and activated TLR2－NF-kB1 signaling pathway to regulate β-defensin 1 expression

“…The colonization of B. subtilis in the ileal mucous membrane enriched bacterial diversity, which improved the phylum and genus of bacterial composition. Genera of lactobacillus, Peptococcus, Butyricicoccus, and Ruminococcaceae_UCG-014 serve probiotic functions for the body, and the proportion of these bacteria increased in chicks receiving B. subtilis and SEBS supplementation [42][43][44]. Meanwhile, the ration of conditioned pathogens or pathogens, such as Escherichia-Shigella, Vibrio cholerae, Salmonella, and Pertussis bacilli, signi cantly declined.…”