Predominant Culturable Bacillus Species in Japanese Arable Soils and Their Potential as Biocontrol Agents

Nishijima, Takanori; Toyota, Koki; Mochizuki, Masahito

doi:10.1264/jsme2.20.61

Cited by 26 publications

(20 citation statements)

References 30 publications

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“…This was expected, since members of the genus Bacillus have been frequently recovered during enrichment of chitin-degrading microorganisms from soil (Cody et al 1989;Nishimura et al 2005). The chitinolytic bacterial community of these soils was clearly dominated by the class Gamaproteobacteria, though Bacillus was found to be one of the major genus, as observed earlier by Smit et al (2001) that the soil with a high content of readily available nutrients showed positive selection for Alpha and Gammaproteobacteria.…”

Section: Discussionsupporting

confidence: 71%

Members of Gammaproteobacteria and Bacilli represent the culturable diversity of chitinolytic bacteria in chitin-enriched soils

Das

Sarma

Chilukoti

et al. 2010

World J Microbiol Biotechnol

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Culturable chitinolytic bacterial diversity was studied in chitin-rich soils collected from two industries involved in chitin production. A total of 27 chitinolytic isolates were isolated among which only 10 showed zone of clearance C4 mm on colloidal chitin agar plate. Using morphological, biochemical and 16S rDNA analysis, isolates were identified as Bacillus, Paenibacillus, Stenotrophomonas and Pseudomonas. Molecular phylogenetic analysis revealed that Gammaproteobacteria and Bacilli were found to be the predominant classes in these chitinenriched soils. Chitinolytic bacterial population densities were significantly high and showed a rather simple community composition dominated by genus Bacillus and Stenotrophomonas (74%). This is the first report on assessing the chitinolytic bacterial diversity of soils from industries involved in chitin production.

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Section: Discussionsupporting

confidence: 71%

Members of Gammaproteobacteria and Bacilli represent the culturable diversity of chitinolytic bacteria in chitin-enriched soils

Das

Sarma

Chilukoti

et al. 2010

World J Microbiol Biotechnol

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“…Por otro lado, a pesar que los valores bajos de pH no son los valores óptimos de crecimiento para Bacillus, estudios previos llevados a cabo con valores de pH bajos en suelo (Nishijima et al, 2005) han demostrado la presencia activa de especies de Bacillus.…”

Section: Resultados Y Discusiónunclassified

CARACTERIZACIÓN FISIOLÓGICA DE CEPAS de Bacillus spp. AISLADAS DE LA RIZÓSFERA DE PAPA (Solanum tuberosum)

Calvo

Zúñiga

2010

Ecol. apl.

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ResumenBacillus es un género altamente presente en la rizósfera de diversos cultivos debido a su capacidad de formación de esporas que le da una ventaja de supervivencia en la rizósfera vegetal. En el presente estudio se realizó una descripción morfológica de las colonias, caracterización fisiológica y bioquímica a 43 aislamientos de Bacillus spp. provenientes de la rizósfera del cultivo de papa de dos zonas altoandinas en Perú. Se encontró que la mayoría de estos aislamientos pertenecían al grupo de "Bacillus subtilis". En cuanto a las pruebas fisiológicas de crecimiento a bajas temperaturas (4ºC, 10 ºC, 15ºC y 20ºC), de las 43 cepas probadas solo una creció a 4ºC, sin embargo, el 98% creció a 10ºC y el 100% a 15 ºC y a 20ºC. Por otro lado, respecto al pH, se observó que 100% de las cepas, crecieron bien en ambos pHs (4 y 5.5), lo que indica una buena adaptación del crecimiento a pH ácidos. La evaluación de los niveles de crecimiento tanto a bajo pH y temperatura baja en el tiempo, revelan un patrón bifásico que se explicaría por pequeños periodos de retraso del crecimiento en condiciones de estrés. Debido a las diferencias de adaptación fisiológica entre cepas de la misma especie se puede sugerir la existencia de una gran diversidad intraespecifica de los Bacillus aislados. Asimismo se encontraron correlaciones entre zonas de aislamientos con temperaturas de suelo más frías y las cepas con mejores niveles de crecimiento a 20ºC. Palabras clave: papa, rizósfera, Bacillus spp., pH, temperatura, diversidad AbstractBacillus is an abundant genus in the rhizosphere of many crops due to its spore formation capacity that gives it an advantage to survive in the rhizosphere. In the present study we worked with 43 Bacillus spp. previously isolated from potato rhizosphere of two highland zones in Peru. We described the morphological characteristic of their colonies and tested them for their physiological and biochemical characterization. Almost all Bacillus strains belong to the "Bacillus subtilis" group. Also acid pH test plate (4 and 5.5) and low temperature plate test (4ºC, 10ºC, 15ºC and 20ºC) were done. Of the 43 tested strains only one grew at 4ºC; therefore, 99% grew at 10ºC, 100% grew at 15ºC and 20ºC. On the other hand, 100% of the strains grew at pH 4 and 5.5, which indicates an adaptation to acid pH. The evaluation of the growth levels at low pH and low temperature in time reveals a two-phase pattern that could be explained by small lag periods in stress conditions. Due to the differences in the physiological adaptation among strains of the same species; the occurrence of a great intraspecific diversity of Bacillus isolated from potato crop rhizosphere can be suggested. We also found correlations between low temperatures in the isolations zones and a better growth level at 20ºC in the plates tested. Key words: potato, rhizosphere, Bacillus spp., pH, temperature, diversity Introducción.Las poblaciones de bacterias en el suelo no se distribuyen al azar. Factores como la composición de los suelos, la materia orgáni...

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“…The results show that 16S rDNA of X-3 and X-6 had 100% identities with B. megaterium (EU627686) and B. cereus (AY129651), respectively. Both Bacillus species had been isolated from arable soils and were shown to have disease control effects in tomato crops (Nishijima et al, 2005).…”

Section: Isolation and Identification Of Antagonistic Endophytic Bactmentioning

confidence: 99%

Endophytic bacterial communities in tomato plants with differential resistance to Ralstonia solanacearum

Feng¹,

YanChang²,

Liu³

2013

Afr. J. Microbiol. Res.

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The endophytic bacterial communities in tomato varieties having differing resistance (or susceptibility) to Ralstonia solanacearum were investigated using both cultivation dependent and independent approaches. Both approaches revealed the differences between resistant (Xiahong-1) and susceptible (Baoshi-5) cultivars in terms of diversity and abundance of endophytic bacteria. The amount of the endogenous bacteria in Xiahong-1 at different growth stages was significantly higher than that in Baoshi-5. Furthermore, there were more culturable and antagonistic endophytic bacteria in Xiahong-1 than that in Baoshi-5. Seven endophytic bacterial genetic groups were identified in Xiahong-1 by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and 16S rDNA sequence, and they were highly similar to Sphingomonas yanoikuya, Pseudomonas pseudoalcaligenes, Serratia marcescens, Bacillus megaterium, Paenibacillus polymyxa, B. pumilus and B. cereus. Four groups were identified in Baoshi-1 which were highly similar to S. yanoikuyae, Pseudomonas fluorescens, Arthrobacter globiformis and Paenibacillus polymyxa. In addition, antagonistic endophytes were identified by 16S rRNA gene analysis, and tested for their abilities to protect tomato plants from infection with R. solanacearum. The relationships between plant resistance and endophytic bacteria diversity are discussed.

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Predominant Culturable Bacillus Species in Japanese Arable Soils and Their Potential as Biocontrol Agents

Cited by 26 publications

References 30 publications

Members of Gammaproteobacteria and Bacilli represent the culturable diversity of chitinolytic bacteria in chitin-enriched soils

Members of Gammaproteobacteria and Bacilli represent the culturable diversity of chitinolytic bacteria in chitin-enriched soils

CARACTERIZACIÓN FISIOLÓGICA DE CEPAS de Bacillus spp. AISLADAS DE LA RIZÓSFERA DE PAPA (Solanum tuberosum)

Endophytic bacterial communities in tomato plants with differential resistance to Ralstonia solanacearum

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