Predominant bacterial species in subgingival plaque in dogs

Dahlén, Gunnar; Charalampakis, Georgios; Abrahamsson, Ingemar; Bengtsson, Lisbeth; Falsen, Enevold

doi:10.1111/j.1600-0765.2011.01440.x

Cited by 42 publications

(52 citation statements)

References 47 publications

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“…Until recently, studies into the canine oral microbiota were limited in their depth and breadth of coverage and constrained by a reliance on culture based methods that did not allow the detection of certain species (Elliott et al, 2005;Hardham et al, 2005;Dahlén et al, 2012). In 2012, Dewhirst et al provided the first in depth analysis of bacterial species present in canine plaque.…”

Section: Introductionmentioning

confidence: 97%

A longitudinal assessment of changes in bacterial community composition associated with the development of periodontal disease in dogs

Wallis¹,

Marshall²,

Colyer³

et al. 2015

Veterinary Microbiology

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Periodontal disease is the most widespread oral disease in dogs. Whilst the involvement of bacteria in the aetiology of periodontitis is well established the role of individual species and their complex interactions with the host is not well understood. The objective of this research was therefore to perform a longitudinal study in dogs to identify the changes that occur in subgingival bacterial communities during the transition from mild gingivitis to the early stages of periodontitis (<25% attachment loss). Subgingival plaque samples were collected from individual teeth of 52 miniature schnauzer dogs every six weeks for up to 60 weeks. The microbial composition of plaque samples was determined using 454-pyrosequencing of the 16S rDNA. A group of aerobic Gram negative species, including Bergeyella zoohelcum COT-186, Moraxella sp. COT-017, Pasteurellaceae sp. COT-080, and Neisseria shayeganii COT-090 decreased in proportion as teeth progressed to mild periodontitis. In contrast, there was less evidence that increases in the proportion of individual species were associated with the onset of periodontitis, although a number of species (particularly members of the Firmicutes) became more abundant as gingivitis severity increased. There were small increases in Shannon diversity, suggesting that plaque community membership remains relatively stable but that bacterial proportions change during progression into periodontitis. This is the first study to demonstrate the temporal dynamics of the canine oral microbiota; it showed that periodontitis results from a microbial succession predominantly characterised by a reduction of previously abundant, health associated taxa.

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Section: Introductionmentioning

confidence: 97%

A longitudinal assessment of changes in bacterial community composition associated with the development of periodontal disease in dogs

Wallis¹,

Marshall²,

Colyer³

et al. 2015

Veterinary Microbiology

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show abstract

“…While there has been much research into the subgingival bacterial microflora in humans, the same remains considerably less explored in dogs, particularly in terms of anaerobic bacterial microflora [12]. Regardless, in the course of numerous studies, various micro-organisms have been isolated that originate from the subgingival region and actively contribute to the onset of periodontitis [26].…”

Section: Introductionmentioning

confidence: 99%

Molecular-level evaluation of selected periodontal pathogens from subgingival regions in canines and humans with periodontal disease

Gołyńska

Polkowska

Bartoszcze-Tomaszewska³

et al. 2017

J Vet Sci

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Dogs commonly serve as a model for various human conditions, including periodontal diseases. The aim of this study was to identify the anaerobic bacteria that colonize the subgingival areas in dogs and humans by using rapid real-time polymerase chain reaction (RT-PCR)-based tests and to compare the results obtained in each species. Bacterial microflora evaluations, both quantitative and qualitative, were performed by applying ready-made tests on twelve dogs and twelve humans. Five samples were collected from each subject's deepest gingival pockets and joined to form a collective sample. The results of the study revealed interspecies similarities in the prevalences of Porphyromonas (P.) gingivalis, Treponema denticola, Tannerella forsythia, and Fusobacterium nucleatum. Red complex bacteria comprised the largest portion of the studied bacterial complexes in all study groups, with P. gingivalis being the most commonly isolated bacterium. The results show similarities in the prevalence of bacterial microflora in dogs and humans. Microbiological analysis of gingival pockets by using rapid real-time PCR-based tests in clinical practice, both veterinary and human, can facilitate the choice of appropriate pharmacological treatment and can provide a basis for subsequent verification of the treatment's effectiveness.

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“…Most of the animals used for research purposes in dentistry were purely for teaching or educational training purposes. [111213141516] In many of the dental research institutes, small animal models were used. Larger animals were reserved as the last choice for validation of newer surgical techniques prior to the use in human beings.…”

Section: Various Animal Models Usedmentioning

confidence: 99%

Ethical guidelines, animal profile, various animal models used in periodontal research with alternatives and future perspectives

Pasupuleti¹,

Shetty²,

Salwaji³

2016

J Indian Soc Periodontol

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Laboratory animal models serve as a facilitator to investigate the etiopathogenesis of periodontal disease, are used to know the efficacy of reconstructive and regenerative procedures, and are also helpful in evaluation of newer therapeutic techniques including laser and implant therapies prior to application in the human beings. The aim of this review is to know the different animal models used in various specialties of dental research and to know the ethical guidelines prior to the usage of experimental models with main emphasis on how to refine, replace, and reduce the number of animal models usage in the laboratory. An online search for experimental animal models used in dental research was performed using MEDLINE/PubMed database. Publications from 2009 to May 2013 in the specialty of periodontics were included in writing this review. A total of 652 references were published in PubMed/MEDLINE databases based on the search terms used. Out of 245 studies, 241 were related to the periodontal research published in English from 2009 to 2013. Relevant papers were chosen according to the inclusion and exclusion criteria. After extensive electronic and hand search on animal models, it has been observed that various animal models were used in dental research. Search on animal models used for dental research purpose revealed that various animals such as rats, mice, guinea pigs, rabbit, beagle dogs, goats, and nonhuman primates were extensively used. However, with the new advancement of ex vivo animal models, it has become easy to investigate disease pathogenesis and to test the efficacy of newer therapeutic modalities with the reduced usage of animal models. This review summarized the large amount of literature on animal models used in periodontal research with main emphasis on ethical guidelines and on reducing the animal model usage in future perspective.

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Predominant bacterial species in subgingival plaque in dogs

Abstract: The predominant cultural subgingival flora in dogs shows great similarities with the subgingival bacteria from humans at the genus level, but distinct differences at the species level; however, a genetic relatedness could be disclosed for most strains investigated.

Cited by 42 publications

References 47 publications

A longitudinal assessment of changes in bacterial community composition associated with the development of periodontal disease in dogs

A longitudinal assessment of changes in bacterial community composition associated with the development of periodontal disease in dogs

Molecular-level evaluation of selected periodontal pathogens from subgingival regions in canines and humans with periodontal disease

Ethical guidelines, animal profile, various animal models used in periodontal research with alternatives and future perspectives

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