Predominance of new G9P[8] rotaviruses closely related to Turkish strains in Nizhny Novgorod (Russia)

Abstract

Genotype G9P[8] rotaviruses are rare in the territory of Russia. They were found in Nizhny Novgorod only in 2011-2012 for the first time, when their proportion was 25.9%. During the next two seasons, G9P[8] strains were detected in only 1.8% of cases. Their proportion substantially increased again in 2014, and they became predominant in the city by 2016. Phylogenetic analysis on the basis of gene VP7 nucleotide sequences showed that this increase was accompanied by the emergence of new strains in the population. These isolates were related to Turkish strains, but not to Russian ones detected earlier.

Phylogenetic analysis of human G9P[8] rotavirus strains circulating in Jiangsu, China between 2010 and 2016

“…In the phylogenetic tree, these 5 strains also showed close similarity with strains from Japan, Thailand, and the US. Interestingly, these results differed from a recent report from Russia, in which all the G9 strains from 2011 to 2016 were clustered in lineage III …”

“…A possible explanation for this might be that lineage P[8]‐3 is more important as an emerging pathogen than other lineages. Our current results are in agreement with previous reports from China and Russia …”

“…Our current results are in agreement with previous reports from China and Russia. 30,31 This study found that changes in amino acid positions, such as (T78S, K), (I91V), (D153N), (T245K), (F587V, I), (S608A), and (R651G) were those that systematically differed compared with B3458. All the amino acid changes of the VP4 gene in the 7 strains were consistent with the recently circulating P [8] strains.…”

“…Interestingly, these results differed from a recent report from Russia, in which all the G9 strains from 2011 to 2016 were clustered in lineage III. 31 Lopez et al 32 previously reported that the VP7 gene had no effect on expression or distribution of RVA, but was important for viral particle maturation. Nine VRs (VR1-9) of VP7 were disparate among different RVA serotypes, and accumulation of genetic shift and genetic drift contributed to the genetic diversity of RVA.…”

Differences in the amino acid composition of the antigen epitopes of the VP7 protein of Russian rotaviruses with the G9 genotype and the vaccine strains RotaTeq, Rotavac, and Rotarix

“…Широко применяют две вакцины: реассортантную пентавалентную вакцину RotaTeq ® (RV5) (Merck&Co. Inc., WestPoint, PA, США), созданную на основе бычьего ротавируса, содержащего в своем составе гены VP7 и VP4 штаммов ротавируса человека генотипов G1, G2, G3, G4 и P [8] соответ-ственно [30] и моновалентную вакцину Rotarix (RV1) (GlaxoSmithKline Biologicals, Rixensart, Бельгия), представляющую собой аттенуированный штамм генотипа G1P [8] ротавируса человека [23]. Кроме этого, существуют вакцины, которые используют на региональном уровне: Rotavin-M1, разработанная на основе штамма G1P [8] [8], генетически родственные штаммам, изолированным на территории Турции, заняли доминирующее положение (45,9%) [26].…”