Predominance of Multidrug-Resistant Gram-Negative Bacteria Isolated from Supermarket Retail Seafood in Japan

Xedzro, Christian; Shimamoto, Toshi; Shimamoto, Tadashi

doi:10.3390/microorganisms11122935

Cited by 2 publications

(1 citation statement)

References 45 publications

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“…Bacteria belonging to the Aeromonas genus are widely distributed and commonly isolated from seafood [ 6 , 79 , 80 ]. Among the most prevalent Aeromonas species isolated from this source are A. salmonicida , A. media , A. bestiarum , A. veronii , A. hydrophila , A. dhakensis and A. caviae [ 81 , 82 , 83 ].…”

Section: Discussionmentioning

confidence: 99%

Aeromonas trota Is Highly Refractory to Acquire Exogenous Genetic Material

Otero-Olarra,

Díaz-Cárdenas,

Aguilera-Arreola

et al. 2024

Microorganisms

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Aeromonas trota is sensitive to most antibiotics and the sole species of this genus susceptible to ampicillin. This susceptibility profile could be related to its inability to acquire exogenous DNA. In this study, A. trota isolates were analyzed to establish their capacity to incorporate foreign DNA. Fourteen strains were identified as A. trota by multilocus phylogenetic analysis (MLPA). Minimal inhibitory concentrations of antibiotics (MIC) were assessed, confirming the susceptibility to most antibiotics tested. To explore their capacity to be transformed, A. trota strains were used as recipients in different horizontal transfer assays. Results showed that around fifty percent of A. trota strains were able to incorporate pBAMD1-2 and pBBR1MCS-3 plasmids after conjugal transfer. In all instances, conjugation frequencies were very low. Interestingly, several isoforms of plasmid pBBR1MCS-3 were observed in transconjugants. Strains could not receive pAr-32, a native plasmid from A. salmonicida. A. trota strains were unable to receive DNA by means of electroporation, natural transformation or vesiduction. These results confirm that A. trota species are extremely refractory to horizontal gene transfer, which could be associated to plasmid instability resulting from oligomerization or to the presence of defense systems against exogenous genetic material in their genomes. To explain the poor results of horizontal gene transfer (HGT), selected genomes were sequenced and analyzed, revealing the presence of defense systems, which could prevent the stable incorporation of exogenous DNA in A. trota.

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Section: Discussionmentioning

confidence: 99%

Aeromonas trota Is Highly Refractory to Acquire Exogenous Genetic Material

Otero-Olarra,

Díaz-Cárdenas,

Aguilera-Arreola

et al. 2024

Microorganisms

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Integrons: Genetic Dynamic Elements in Aeromonas

Rojas-Rios,

Curiel-Quesada,

Pérez-Valdespino

2024

Aeromonas - An Ubiquitous Bacteria That Infects Humans and Animals [Working Title]

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Integrons are genetic elements able to incorporate, express, and exchange cassettes. Cassette exchanges are mediated by an integrase that excises and reinserts them via site-specific recombination between flanking att sequences. Cassettes lack for the most part a promoter, and their excision and reinsertion at the attI site, downstream a Pc promoter, render them transcriptionally active. This work documents the occurrence of complete integrons or parts of them in Aeromonas and examines the dynamics of these elements. Class 1 integrons linked to antibiotic-resistant cassettes were the most abundant in strains isolated from environmental and clinical samples. Detection of class 2 and 3 integrons was infrequent. Only one report of a class 4-like integron in Aeromonas sp. genomospecies paramedia was found, but a search of these integrons in Aeromonas genomes revealed that class 4-like integrons were the second most abundant after those belonging to class 1. In addition to complete integrons, clusters of attC sites lacking a neighboring integron-integrase (CALINs), single attC sites lacking integron-integrase (SALINs), and orphan integrase genes were found in chromosomes, and a low percentage in Aeromonas plasmids. Concerning the integron behavior, it is known to be regulated by SOS response and could be also controlled by host specific factors.

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Predominance of Multidrug-Resistant Gram-Negative Bacteria Isolated from Supermarket Retail Seafood in Japan

Cited by 2 publications

References 45 publications

Aeromonas trota Is Highly Refractory to Acquire Exogenous Genetic Material

Aeromonas trota Is Highly Refractory to Acquire Exogenous Genetic Material

Integrons: Genetic Dynamic Elements in Aeromonas

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