Predominance and genetic diversity of community- and hospital-acquired CTX-M extended-spectrum β-lactamases in York, UK

Munday, Craig Joe; Whitehead, G. M.; Todd, Neil; Campbell, Margaret A.; Hawkey, Peter M.

doi:10.1093/jac/dkh397

Cited by 107 publications

(61 citation statements)

References 30 publications

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“…The increasing prevalence of ESBL-positive Entero bacteriaceae in the GIT of community subjects has also been reported in other European countries such as Spain and Great Britain 8,23 . Most frequently, Escherichia coli isolates producing CTX-M beta-lactamases are identified 24,25 .…”

Section: Discussionsupporting

confidence: 55%

Carriage of ESBL- and AmpC-positive Enterobacteriaceae in the gastrointestinal tract of community subjects and hospitalized patients in the Czech Republic

Husičková¹,

Čekanová²,

Chromá³

et al. 2012

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Aim. The study aimed at analyzing ESBL-and AmpC-positive Enterobacteriaceae in the gastrointestinal tracts of university hospital inpatients and persons from the Olomouc Region community, and comparing the results with data from 2007. Methods. Bacteria were isolated from rectal swabs inoculated onto the ChromID TM ESBL selective medium (bioMérieux). Production of ESBL-type beta-lactamases was confirmed by the modified double-disk synergy test and AmpC enzyme production was detected by the AmpC disk test. ESBL-and AmpC-positive isolates were subjected to basic genetic analysis aimed at detecting the bla TEM , bla SHV , bla CTX-M and bla AmpC genes. Results. Over the study period (1 March 2010 -1 May 2010), a total of 1,279 rectal swabs (70.4% of community subjects) were analyzed on the above medium. The prevalence rates of ESBL-positive Enterobacteriaceae were 8.2% in hospitalized patients and 3.2% in community subjects. Production of the AmpC enzyme was detected in 1.1% of bacterial isolates from the community and in one (0.3%) hospital isolate. Among ESBL, the most frequent genes encoding enzymes were from the CTX-M-1-like genes. Detected AmpC beta-lactamases belonged to the CIT, DHA and EBC groups. Conclusion. When compared with the year 2007, the rates of carriers of ESBL-positive bacteria increased in both hospitalized patients (from 3% to 8%) and community subjects (from 1% to 3%) in 2010. Given the fact that production of extended-spectrum beta-lactamases is clinically significant, knowing the epidemiological situation is very important for selecting adequate antibiotic therapy.

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Section: Discussionsupporting

confidence: 55%

Carriage of ESBL- and AmpC-positive Enterobacteriaceae in the gastrointestinal tract of community subjects and hospitalized patients in the Czech Republic

Husičková¹,

Čekanová²,

Chromá³

et al. 2012

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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“…The DNA sequence identity of all bla CTX-M genes of clinical isolates and control strains was confirmed by bidirectional sequencing of their PCR products using Beckman CEQ2000 protocols. The initial sample screening and antibiotic susceptibility testing were performed as described previously (Munday et al, 2004).…”

Section: Methodsmentioning

confidence: 99%

“…has been reported (Arpin et al, 2003;Chanawong et al, 2002;Colom et al, 2003;Karim et al, 2001;Munday et al, 2004), although, so far, strains carrying multiple bla CTX-M genes have not been observed. However, given the nature of involvement of different genetic elements in the mobilization of bla genes, it is not impossible that dual or multiple expression of bla CTX-M genes within the same isolate may arise.…”

Section: Evaluation Of Multiplex Pcr With Clinical Isolatesmentioning

confidence: 99%

“…No group 2 or group 8 clinical isolates were available to us. All of the isolates were characterized in our laboratory previously by a standard PCR-based method using group-specific primers in four separate PCRs (Munday et al, 2004). Multiplex PCR testing of these 62 clinical isolates was performed in a blinded fashion.…”

Section: Evaluation Of Multiplex Pcr With Clinical Isolatesmentioning

confidence: 99%

“…In the UK, the first report was of a CTX-M-9-producing isolate (group 9) of Klebsiella oxytoca in Leeds in 2000 (Alobwede et al, 2003); subsequently, CTX-M-26 (group 25/26) was recovered from an outbreak in City Hospital, Birmingham (Brenwald et al, 2003). CTX-M-9, -14 (group 9) and -15-producing isolates (group 1) were found in both the community and hospitals in York (Munday et al, 2004). A more recent surveillance study from 42 centres showed that CTX-M-15-producing Escherichia coli were widely scattered throughout the UK (Woodford et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rapid and simple detection of bla CTX−M genes by multiplex PCR assay

Ensor

Gossain

et al. 2005

Journal of Medical Microbiology

111

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A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of bla CTX-M genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified bla CTX-M genes with 100 % accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.

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Antimicrobial Resistance: Preventable or Inevitable?

Aksoy

Tanrıöver

Ünal

2008

Antibiotic Policies: Fighting Resistance

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Predominance and genetic diversity of community- and hospital-acquired CTX-M extended-spectrum β-lactamases in York, UK

Abstract: The overall prevalence of ESBL in isolates of Enterobacteriaceae from York was 1.9%. ESBL-producing isolates were found in both the community and hospital, with the CTX-M type most common. This is also the first report of an ESBL-producing Salmonella in the UK.

Cited by 107 publications

References 30 publications

Carriage of ESBL- and AmpC-positive Enterobacteriaceae in the gastrointestinal tract of community subjects and hospitalized patients in the Czech Republic

Carriage of ESBL- and AmpC-positive Enterobacteriaceae in the gastrointestinal tract of community subjects and hospitalized patients in the Czech Republic

Rapid and simple detection of bla CTX−M genes by multiplex PCR assay

Antimicrobial Resistance: Preventable or Inevitable?

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